Three automated feeding strategies of natural complex nutrients utilizing on-line turbidity values in fed-batch culture: A case study on the cultivation of a marine microorganism

Hibino, Wataru; Kadotani, Yasuyuki; Kominami, Minoru; Yamané, Tsuneo

doi:10.1016/0922-338x(93)90093-n

Cited by 11 publications

(3 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This should be mainly ascribed to the overfeeding of ammonium in the later phase of cultivation. Similar results were found in the fed-batch cultivations of Alteromonas putrefaciens [18], which showed that the cell yield from the nutrients supplied to the culture system could be raised by automated feeding nutrient utilizing the online OD values. It was demonstrated that the OD-based feedback feeding method could improve the nitrogen source utilization efficiency by feeding nutrient approximately to the stoichiometric ratio of nutrient consumption.…”

Section: Comparison Of Different Feeding Methodssupporting

confidence: 79%

An Optical-Density-Based Feedback Feeding Method for Ammonium Concentration Control in Spirulina platensis Cultivation

Bao

Wen²,

Cong³

et al. 2012

J. Microbiol. Biotechnol.

View full text Add to dashboard Cite

Cultivation of Spirulina platensis using ammonium salts or wastewater containing ammonium as alternative nitrogen sources is considered as a commercial way to reduce the production cost. In this research, by analyzing the relationship between biomass production and ammonium-N consumption in the fed-batch culture of Spirulina platensis using ammonium bicarbonate as a nitrogen nutrient source, an online adaptive control strategy based on optical density (OD) measurements for controlling ammonium feeding was presented. The ammonium concentration was successfully controlled between the cell growth inhibitory and limiting concentrations using this OD-based feedback feeding method. As a result, the maximum biomass concentration (2.98 g/l), productivity (0.237 g/l•d), nitrogen-to-cell conversion factor (7.32 gX/gN), and contents of protein (64.1%) and chlorophyll (13.4 mg/g) obtained by using the OD-based feedback feeding method were higher than those using the constant and variable feeding methods. The OD-based feedback feeding method could be recognized as an applicable way to control ammonium feeding and a benefit for Spirulina platensis cultivations.

show abstract

Section: Comparison Of Different Feeding Methodssupporting

confidence: 79%

An Optical-Density-Based Feedback Feeding Method for Ammonium Concentration Control in Spirulina platensis Cultivation

Bao

Wen²,

Cong³

et al. 2012

J. Microbiol. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…The increase of the pH-signal was converted in an increase of the feed rate of fresh medium. A more complex stat is used in the cultivation of Alteromonas putrefaciens (Hibino et al, 1993). In this stat, the yield of biomass on substrate was imposed on the culture.…”

Section: Data Handling In Isa S88-based Mmi-systemsmentioning

confidence: 99%

Untitled

et al. 1997

View full text Add to dashboard Cite

The production of biologicals is subject to strict governmental regulations. These are drawn up in current good manufacturing practices (cGMP), a.o. by the U.S. Food and Drug Administration. To implement cGMP in a production facility, plant automation becomes an essential tool. For this purpose Manufacturing Execution Systems (MES) have been developed that control all operations inside a production facility. The introduction of these recipe-driven control systems that follow ISA S88 standards for batch processes has made it possible to implement cGMP regulations in the control strategy of biological production processes. Next to this, an MES offers additional features such as stock management, planning and routing tools, process-dependent control, implementation of software sensors and predictive models, application of historical data and on-line statistical techniques for trend analysis and detection of instrumentation failures. This paper focuses on the development of new production strategies in which cGMP guidelines are an essential part.

show abstract

“…Different variables have been used for this purpose in systems without cell retention. Typical examples are dissolved oxygen (DO) (Konstantinov et al, 1990; Jin et al, 1992), pH (Ishizaki et al, 1994), the glucose concentration, and in some cases, the cell concentration itself (Hibino et al, 1993). While any of these variables can be used to close the control loop, the selection of the most appropriate one is of critical importance.…”

Section: Introductionmentioning

confidence: 99%

Control of Long-Term Perfusion Chinese Hamster Ovary Cell Culture by Glucose Auxostat

Konstantinov

Tsai

Moles³

et al. 1996

Biotechnol. Prog.

View full text Add to dashboard Cite

The strategies for control of the feed rate in high-density perfusion cultures of animal cells are limited to several simple schemes. While in an industrial environment simplicity is seen as a major advantage, the need for more elaborate closed-loop control methods that can improve process stability in long-term continuous cultures is also well understood. What has prevented the application of the advanced control strategies known from theory is the lack of reliable real-time information that can be used to close the feedback loop. Among the variables that are appropriate for direct feedback control of the perfusion rate, high priority should be given to the glucose concentration. Unlike some other environmental variables, such as dissolved oxygen and pH, it provides unambiguous information which facilitates the selection of the right feed rate. The present paper describes the application of a closed loop control scheme, known as a "glucose-stat", to the long-term cultivation of Chinese hamster ovary cells in a high-density (35-40 million cells/mL) perfusion process. The monitoring and control system worked successfully for more than 2.5 months without any signs of performance degradation. In targeting industrial application, issues such as reliability, sterility, and accuracy, are given high priority. The implementation of the glucose monitoring system, which is the main part of the control complex, is addressed in details. The performance of the perfusion culture was evaluated at four different glucose set points, providing essential information about process optimization. It became evident that the perfusion culture was operated in the so-called "high-gain" zone (where the system is highly sensitive to the dilution rate), which justifies the application of a feedback control. The on-line glucose concentration was also used by an embedded expert system which drove the process through the batch and the perfusion phase, achieving total computer control of the feed rate. In summary, the proposed glucose monitoring and control technique proved to be a reliable biotechnology tool which can be applied with confidence at an industrial scale to either microbial or mammalian cell cultures.

show abstract

Three automated feeding strategies of natural complex nutrients utilizing on-line turbidity values in fed-batch culture: A case study on the cultivation of a marine microorganism

Cited by 11 publications

References 4 publications

An Optical-Density-Based Feedback Feeding Method for Ammonium Concentration Control in Spirulina platensis Cultivation

An Optical-Density-Based Feedback Feeding Method for Ammonium Concentration Control in Spirulina platensis Cultivation

Untitled

Control of Long-Term Perfusion Chinese Hamster Ovary Cell Culture by Glucose Auxostat

Contact Info

Product

Resources

About