Thirteen new dinucleotide microsatellites in Alpaca

Obreque, Victor; Mancilla, Raúl; García-Huidobro, J; Cothran, E. Gus; Hinrichsen, Patricio

doi:10.1046/j.1365-2052.1999.00526-19.x

Cited by 16 publications

(11 citation statements)

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“…The low fixation indices for these loci confirm this interpretation of the data (F=0.004; SE=0.036) and suggest that the microsatellite panel used is suitable for genetic diversity studies. In comparison with the data collected and analyzed by the ARI since 1998 and by several other authors, the sample in this study shows the presence of a high number of alleles, matching the whole allelic range described in previous publications (Obreque et al, 1999;Penedo et al, 1999;McPartlan et al, 1998). Values for the polymorphic information content (PIC=0.746, SE=0.033) were also in line with previous findings.…”

Section: Discussionmentioning

confidence: 79%

“…The continuous and ongoing effort in sequencing the alpaca genome will rapidly lead to a much larger set of markers, as demonstrated by a number of studies published in the past decade (Obreque et al, 1999;Penedo et al, 1999;McPartlan et al, 1998). Reed and Chaves (2008) report an additional 1516 potential loci by blasting bos taurus SSRs and Munyard et al (2009) have recently found a set of 9 tetranucleotide markers.…”

Section: Population Genetics By Means Of Microsatellite Analysismentioning

confidence: 99%

“…The null hypothesis was tested for Ho (P=0.69), He (P=0.61), F (P=0.95), Ne (P=0.69), PIC (P=0.61) and Na (P=0.37). Genetic variability (Obreque et al, 1999;Penedo et al, 1999;McPartlan et al, 1998); Ne, number of effective alleles; Ho, observed heterozygosity; He, expected heterozygosity; UHe, unbiased expected heterozygosity = [2N / (2N-1)] * He; F, fixation index; PIC, polymorphism information content; °Ho, He, UHe and F not shown for X-linked YWLL43 locus.…”

Section: Heterozygosis Polymorphism Information Content and Fixationmentioning

confidence: 99%

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Analysis of genetic distance between Peruvian Alpaca (Vicugna Pacos) showing two distinct fleece phenotypes, Suri and Huacaya, by means of microsatellite markers

Manna

Terza

Ghezzi

et al. 2011

Italian Journal of Animal Science

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Section: Discussionmentioning

confidence: 79%

Section: Population Genetics By Means Of Microsatellite Analysismentioning

confidence: 99%

Section: Heterozygosis Polymorphism Information Content and Fixationmentioning

confidence: 99%

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Analysis of genetic distance between Peruvian Alpaca (Vicugna Pacos) showing two distinct fleece phenotypes, Suri and Huacaya, by means of microsatellite markers

Manna

Terza

Ghezzi

et al. 2011

Italian Journal of Animal Science

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“…1998b; Obreque et al . ; Bustamante et al . ), showed a larger number of alleles in our Bolivian resource population.…”

Section: Discussionmentioning

confidence: 99%

Analysis of genetic diversity in Bolivian llama populations using microsatellites

Barreta

Gutiérrez-Gil

Iñiguez³

et al. 2012

J Animal Breeding Genetics

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South American camelids (SACs) have a major role in the maintenance and potential future of rural Andean human populations. More than 60% of the 3.7 million llamas living worldwide are found in Bolivia. Due to the lack of studies focusing on genetic diversity in Bolivian llamas, this analysis investigates both the genetic diversity and structure of 12 regional groups of llamas that span the greater part of the range of distribution for this species in Bolivia. The analysis of 42 microsatellite markers in the considered regional groups showed that, in general, there were high levels of polymorphism (a total of 506 detected alleles; average PIC across per marker: 0.66), which are comparable with those reported for other populations of domestic SACs. The estimated diversity parameters indicated that there was high intrapopulational genetic variation (average number of alleles and average expected heterozygosity per marker: 12.04 and 0.68, respectively) and weak genetic differentiation among populations (FST range: 0.003-0.052). In agreement with these estimates, Bolivian llamas showed a weak genetic structure and an intense gene flow between all the studied regional groups, which is due to the exchange of reproductive males between the different flocks. Interestingly, the groups for which the largest pairwise FST estimates were observed, Sud Lípez and Nor Lípez, showed a certain level of genetic differentiation that is probably due to the pattern of geographic isolation and limited communication infrastructures of these southern localities. Overall, the population parameters reported here may serve as a reference when establishing conservation policies that address Bolivian llama populations.

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“…1 Habitat of Bikaneri, Jaisalmeri, Kachchhi and Mewari breeds of camel in India microsatellite primer pairs (Obreque et al 1998(Obreque et al , 1999Lang et al 1996;Penedo et al 1998Penedo et al , 1999aMariasegaram et al 2002) were selected for the present study. PCR amplifications were carried out in 25-μl reactions containing 50 ng DNA, 5 pmol each primer (Geneset, Singapore and Genetix, India), 1 U Taq DNA polymerase, 0.2 mM each dNTP, 2.5 μl 10X Taq DNA polymerase buffer containing 10 mM Tris-HCl (pH 9.0), 1.5 mM MgCl 2 , 50 mM KCl and 0.01 % gelatin.…”

Section: Samplingmentioning

confidence: 99%

Genetic and demographic bottleneck analysis of Indian camel breeds by microsatellite markers

Mehta

2014

Trop Anim Health Prod

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The genetic and demographic bottleneck analysis of Indian camel breeds was carried out utilizing 40 microsatellite markers. Allelic polymorphism was observed at 20 loci in the Indian dromedary breeds. A total of 66 alleles were scored. The average number of alleles, expected heterozygosity and polymorphic information content were, respectively, 3.25 ± 0.27, 0.56 ± 0.04 and 0.49 ± 0.04 in Bikaneri; 3.25 ± 0.25, 0.53 ± 0.03 and 0.46 ± 0.03 in Jaisalmeri; 3.0 ± 0.21, 0.53 ± 0.04 and 0.45 ± 0.03 in Kachchhi and 3.1 ± 0.19, 0.51 ± 0.03 and 0.44 ± 0.03 in Mewari breed. Higher genetic variation was observed in most numerous Bikaneri breed. Genetic distances were least between the breed pair Bikaneri and Jaisalmeri which was closely placed with the Kachchhi breed. The Mewari camels had relatively higher genetic distance from the other three Indian dromedary breeds. The bottleneck analysis revealed the presence of genetic bottleneck in all four breeds of Indian dromedary. However, the qualitative graphical method resulted in normal L-shaped distribution of allele frequencies in Jaisalmeri breeds and shifted mode in Bikaneri, Kachchhi and Mewari breeds. The demographic bottleneck analysis revealed minimum reduction (-9.65 %) in the population of camels in Jaisalmeri breeding tract as compared to that of Bikaneri (-14.18 %), Kachchhi (-27.78 %) and Mewari (-32 %) breeding tracts. Conclusively, the genetic bottleneck analysis could explain the demographic bottleneck in the Indian dromedary populations. Therefore, appropriate conservation and improvement efforts are needed in all four dromedary breeds with immediate attention on Mewari and Kachchhi breeds. The present study is the first report in demonstrating the genetic basis of demographic bottleneck in the Indian dromedary populations.

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Thirteen new dinucleotide microsatellites in Alpaca

Cited by 16 publications

References 0 publications

Analysis of genetic distance between Peruvian Alpaca (Vicugna Pacos) showing two distinct fleece phenotypes, Suri and Huacaya, by means of microsatellite markers

Analysis of genetic distance between Peruvian Alpaca (Vicugna Pacos) showing two distinct fleece phenotypes, Suri and Huacaya, by means of microsatellite markers

Analysis of genetic diversity in Bolivian llama populations using microsatellites

Genetic and demographic bottleneck analysis of Indian camel breeds by microsatellite markers

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