Third-harmonic generation microscopy by use of a compact, femtosecond fiber laser source

Millard, Andrew C.; Wiseman, Paul W.; Fittinghoff, D. N.; Wilson, Kent R.; Squier, Jeffrey A.; Müller, Markus

doi:10.1364/ao.38.007393

Cited by 99 publications

(85 citation statements)

References 12 publications

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“…Erythrocytes elicit a particularly strong THG signal that originates from their cellular membrane (Millard et al, 1999). An additional signal, with variable efficiency, originates from basement membranes lining the vessels (Rehberg et al, 2011;Weigelin et al, 2012).…”

Section: Blood Vessel Structure and Functionmentioning

confidence: 99%

Third harmonic generation microscopy of cells and tissue organization

Weigelin

Bakker

Friedl

2016

Journal of Cell Science

167

147

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The interaction of cells within their microenvironmental niche is fundamental to cell migration, positioning, growth and differentiation in order to form and maintain complex tissue organization and function. Third harmonic generation (THG) microscopy is a label-free scatter process that is elicited by water-lipid and water-protein interfaces, including intra-and extracellular membranes, and extracellular matrix structures. In applied life sciences, THG delivers a versatile contrast modality to complement multi-parameter fluorescence, second harmonic generation and fluorescence lifetime microscopy, which allows detection of cellular and molecular cell functions in threedimensional tissue culture and small animals. In this Commentary, we review the physical and technical basis of THG, and provide considerations for optimal excitation, detection and interpretation of THG signals. We further provide an overview on how THG has versatile applications in cell and tissue research, with a particular focus on analyzing tissue morphogenesis and homeostasis, immune cell function and cancer research, as well as the emerging applicability of THG in clinical practice.

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Section: Blood Vessel Structure and Functionmentioning

confidence: 99%

Third harmonic generation microscopy of cells and tissue organization

Weigelin

Bakker

Friedl

2016

Journal of Cell Science

167

147

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“…Nonlinear optical techniques such as multiphoton absorption, 66,67 second- 68,69 and third- [70][71][72] harmonic generation, and coherent anti-Stokes Raman scattering [73][74][75][76][77] (CARS) are all being used to great advantage in microscopy. Due to their nonlinear intensity dependence, all of these techniques offer exceptional three-dimensional spatial resolution while generally not affecting the sample being studied anywhere except in the focal region.…”

Section: T 1 T Fill T 2 > T Fill T 3 < T Fill T Break < Tmentioning

confidence: 99%

Optical Kerr Effect Spectroscopy of Simple Liquids

2008

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Optical Kerr effect (OKE) spectroscopy has become a prominent nonlinear optical technique for studying liquids, which allows for the direct, time-resolved probing of collective orientational diffusion as well as Raman-active intermolecular and intramolecular modes.The temperature-dependent orientational dynamics of 1, n-dicyano n-alkane liquids ranging from dicyanomethane to 1,8-dicyanooctane has been investigated by ultrafast OKE spectroscopy. The dependence of the reorientational times on temperature and viscosity is consistent with the molecules adopting a largely extended structure in the liquid state, with a preference for gauche conformations at the methylenes bonded to the cyanide groups. The data are also suggestive of temperature-dependent, collective structural rearrangements in these liquids.Ultrafast OKE spectroscopy has also been used to study the intermolecular dynamics of aromatic liquids. A model that links the differences in the OKE spectra to corresponding differences in the local ordering of the liquids has been proposed previously based on the temperature-dependent OKE study of five aromatic liquids.The spectra of some other aromatic liquids such as pyridine, pyridine-d5, 2,4,6-trifluoropyridine and 1,3,5-tris(trifluoromethyl) benzene has been obtained to test this model, and the relative importance of molecular shape and electrostatic forces in determining the form of the OKE reduced spectral density for such liquids has been realized. It has recently been shown that liquid tetrahydrofuran (THF) has an unusual structure that features voids of significant dimension. Such voids should affect other observable properties of this liquid. Temperature-dependent, optical Kerr effect spectra for THF and a number of related liquids (furan, cyclopentane, tetrahydropyran, cyclohexane, diethyl ether, hexamethylphosphoramide and n-pentane) has been obtained to test whether the shape of the spectra can be used to reveal the presence of sizeable voids in liquids. Liquid under tension is another interesting system to study:A method based on Berthelot tube technique has been developed to hold benzene and acetonitrile under tension successfully.A new scheme for measuring different tensor elements of the OKE response is developed. A dual-ring, polarization dependent Sagnac interferometer is used to create two co-propagating probe pulses that arrive at the sample at different times but that reach the detector simultaneously and collinearly. The tensor element of the response that is measured is determined by the polarization of the pump pulse. By controlling the relative timing of the probe pulses it is also possible to perform optical subtraction of two different tensor elements of the response at two different times, a strategy that can be used to enhance or suppress particular contributions to the OKE response. OPTICAL KERR EFFECT SPECTROSCOPY OF SIMPLE LIQUIDS

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“…In addition to the conventional Ti:sapphire lasers, highpower, ultrashort pulse bulk solid-state or fiber lasers are now available commercially and are frequently used for nonlinear microscopic imaging (Millard et al 1999;Prent et al 2008;Pegoraro et al 2009a, b). Most commonly, Er 3+ (1540 nm) or Yb 3+ (1030 nm) doped glasses make up the core of these fibers (or bulk crystals) and constitute the gain medium.…”

Section: Laser Sourcesmentioning

confidence: 99%

Nonlinear optical microscopy in decoding arterial diseases

Ridsdale

Mostaço-Guidolin

et al. 2012

Biophys Rev

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Pathological understanding of arterial diseases is mainly attributable to histological observations based on conventional tissue staining protocols. The emerging development of nonlinear optical microscopy (NLOM), particularly in second-harmonic generation, two-photon excited fluorescence and coherent Raman scattering, provides a new venue to visualize pathological changes in the extracellular matrix caused by atherosclerosis progression. These techniques in general require minimal tissue preparation and offer rapid three-dimensional imaging. The capability of label-free microscopic imaging enables disease impact to be studied directly on the bulk artery tissue, thus minimally perturbing the sample. In this review, we look at recent progress in applications related to arterial disease imaging using various forms of NLOM.

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Third-harmonic generation microscopy by use of a compact, femtosecond fiber laser source

Cited by 99 publications

References 12 publications

Third harmonic generation microscopy of cells and tissue organization

Third harmonic generation microscopy of cells and tissue organization

Optical Kerr Effect Spectroscopy of Simple Liquids

Nonlinear optical microscopy in decoding arterial diseases

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