Thiol redox state (TRS) and oxidative stress in the mouse hippocampus after pentylenetetrazol-induced epileptic seizure

Patsoukis, Nikolaos; Zervoudakis, George; Panagopoulos, Nikolaos T.; Georgiou, Christos D.; Angelatou, Fevronia; Matsokis, Nikolaos

doi:10.1016/j.neulet.2003.10.080

Cited by 188 publications

(117 citation statements)

References 15 publications

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“…It affects GABAergic and Glutamergic systems in many brain regions including hippocampus. [34] PTZ application causes hippocampal atrophy in rats. Selective neuronal loss and astocytosis in hippocampus are observed in PTZ administered rats.…”

Section: Number Of Animalsmentioning

confidence: 97%

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Pentylenetetrazol Kindling Epilepsy Model

Erkeç¹,

Arıhan²

2015

Epilepsi

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SummaryEpilepsy is one of the most common neurologic disorders affecting approximately 1% of the general population, and no alleviation was achieved in one third of the patients medicated with antiepileptic drugs. Current treatments of epilepsy are symptomatic and have more anti-seizure effects than antiepileptic effects. These therapies do not cure epilepsy. Basic mechanisms of epilepsy have not entirely been clarified yet. Using seizure and epilepsy animal models, our comprehension about basic mechanisms underlying epileptogenesis has improved. In addition, animal models of epilepsy and seizures are very useful in the discovery and development of new antiepileptic drugs. Pentylenetetrazole (PTZ) is widely used in antiepileptic drug discovery studies, and PTZ kindling model is very important to understand the pathophysiology of epilepsy. In this review, current information about PTZ kindling model was given in this aspect.Key words: Chemical kindling; epilepsy; experimental animal models; pentylenetetrazole; rat. ÖzetEpilepsi genel popülasyonun yaklaşık %1'ini etkileyen en yaygın nörolojik düzensizliklerden biridir. Antiepileptik ilaç tedavisi gören hastaların üçte birinde hiçbir hafifleme elde edilememektedir. Epilepsi için kullanılan güncel tedaviler semptomatiktir ve antiepileptik etkiden ziyade anti nöbet etkilidir. Bu tedaviler epilepsiyi tedavi etmemektedir. Epilepsinin temel mekanizmaları da henüz tam olarak anlaşılamamıştır. Nö-bet ve epilepsi hayvan modelleri kullanılarak epilepsinin temelinde yatan mekanizmaları kavrayışımız ilerlemektedir. Ayrıca epilepsi ve nöbet hayvan modelleri yeni antiepileptik ilaç keşfi ve gelişimi bakımından kullanışlıdırlar. Pentilentetrazol (PTZ), antiepileptik ilaç keşif çalışmala-rında yaygın biçimde kullanılmaktadır ve PTZ tutuşma modeli epilepsinin patofizyolojisinin anlaşılması bakımından oldukça önemlidir. Bu

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Section: Number Of Animalsmentioning

confidence: 97%

“…Findings of Patsoukis et al show a strong correlation between PTZ administration and oxidative stress. [34] PTZ kindling causes alterations in antioxidant defense systems of the brain. [20] Oxidative stress is also present in other experimental epilepsy models as well as in PTZ kindling model.…”

Section: Alterations In Gabaergic Systemsmentioning

confidence: 99%

Pentylenetetrazol Kindling Epilepsy Model

Erkeç¹,

Arıhan²

2015

Epilepsi

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“…Protein carbonyl determination was based on the method of Patsoukis et al (37) In this assay, 50 μl of 20% TCA was added to 50 μl of plasma or tissues homogenate (diluted 1:2), and this mixture was incubated in an ice-bath for 15 min and then centrifuged (15,000 × g, 5 min, 4˚C). The supernatant was discarded, and 500 μl of 10 mmol/l 2,4-dinitrophenylhydrazine (in 2.5 N HCl) per sample (500 μl of 2.5 N HCl for the blank) was added to the pellet.…”

Section: Determination Of Antioxidant Activity Of Feed Rations: 22-dmentioning

confidence: 99%

Novel Feed Including Olive Oil Mill Wastewater Bioactive Compounds Enhanced the Redox Status of Lambs

Makri

Kafantaris

Savva

et al. 2018

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“…PTZ induced seizures are still the most widely used animal seizure models employed in the research for epilepsy and new antiepileptic drugs (8). It is reported that free radical generation plays a crucial role in neuronal cell death in the PTZ induced seizures in rats (9). Some studies suggested that proinflammatory molecules (e.g.proteolytic enzymes, reactive oxygen species or nitric oxide) may potentiate the damage to brain and peripheral tissues in epilepsy (10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

EffectsofLuteolinonLiver,KidneyandBraininPentylentetrazol-Induced Seizures: Involvement of Metalloproteinases and NOS Activities

Birman¹,

Dar²,

Kapucu³

et al. 2012

Balkan Med J

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Objective: Flavonoids are an important group of recognized antioxidants in plants. Luteolin (LUT) is a natural flavonoid in the plant kingdom. This study was aimed to investigate the effects of the LUT in the liver, kidney and brain of pentylentetrazol (PTZ)-induced seizure and the relationship between nitric oxide synthases (iNOS, eNOS) and matrix metalloproteinases (MMP2, MMP9).Materials and Methods: LUT (10 mg/kg) was given intraperitoneally during two weeks prior to seizure induction. A single dose PTZ 80 mg/kg i.p. was administered and seizures were observed and evaluated with regard to latency, frequency and stage for one hour.Results: Seizure frequen cy after PTZ administration was significantly decreased in LUT pretreated rats (p<0.05). An increase of immunhistochemical reactions of iNOS and MMP2, but a decrease of eNOS activity, were observed in rat hippocampus and peripheral tissues during the PTZ induced seizures. LUT pretreatment reversed the iNOS and MMP2 activity to the control levels and significantly increased the eNOS activity (p<0.001).Conclusion: LUT seems to have an effective role in reducing the seizure frequency and a protective role on peripheral organ injury in animal models of seizure. The protective effect of LUT in seizures and the seizure induced peripheral tissue damage warrant further investigations.

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Thiol redox state (TRS) and oxidative stress in the mouse hippocampus after pentylenetetrazol-induced epileptic seizure

Cited by 188 publications

References 15 publications

Pentylenetetrazol Kindling Epilepsy Model

Pentylenetetrazol Kindling Epilepsy Model

Novel Feed Including Olive Oil Mill Wastewater Bioactive Compounds Enhanced the Redox Status of Lambs

EffectsofLuteolinonLiver,KidneyandBraininPentylentetrazol-Induced Seizures: Involvement of Metalloproteinases and NOS Activities

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