Thiol–ene micropillar array electrospray ionization platform for zeptomole level bioanalysis

Lipponen, Katriina; Tähkä, Sari; Sikanen, Tiina; Jokinen, Ville; Tatikonda, Anand Kumar; Franssila, Sami; Kostiainen, Risto; Kotiaho, Tapio

doi:10.1039/c7an00544j

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…18 To increase detection sensitivity (and circumvent any issues and challenges with optical detection), emitters for electrospray ionization mass spectrometry (ESI-MS) were developed using replica molding of TE. 21,99,122 Initially, the emitter taper was made in 2D, but for robust and long-term spray stabilities sharp emitter apexes are desired, and an improved version with a three-dimensional tapered geometry was developed (Figure 6C). 21 These 3D emitters lasted for more than a month before mechanical deterioration and showed good spray stabilities for at least 3 h, providing a relative standard deviation of 8% for the baseline over 15 min.…”

Section: ■ Examples Of Te-based Microfluidic Devicesmentioning

confidence: 99%

Thiol–Ene Based Polymers as Versatile Materials for Microfluidic Devices for Life Sciences Applications

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Geczy

Häfeli

et al. 2020

ACS Appl. Mater. Interfaces

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While there is a steady growth in the number of microfluidics applications, the search for an optimal material that delivers the diverse characteristics needed for the numerous tasks is still nowhere close to being settled. Often overlooked and still underrepresented, the thiol−ene family of polymer materials has an enormous potential for applications in organs-on-a-chip, droplet productions, microanalytics, and point of care testing. In this review, the main characteristics of the thiol−ene materials are given, and advantages and drawbacks with respect to their potential in microfluidic chip fabrication are critically assessed. Select applications, which exploit the versatility of the thiol−ene polymers, are presented and discussed. It is concluded that, in particular, the rapid prototyping possibility combined with the material's resulting mechanical strength, solvent resistance, and biocompatibility, as well as the inherently easy surface functionalization, are strong factors to make thiol−ene polymers strong contenders for promising future materials for many biological, clinical, and technical labon-a-chip applications.

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Section: ■ Examples Of Te-based Microfluidic Devicesmentioning

confidence: 99%

Thiol–Ene Based Polymers as Versatile Materials for Microfluidic Devices for Life Sciences Applications

Sticker

Geczy

Häfeli

et al. 2020

ACS Appl. Mater. Interfaces

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“…We speculate that this is due to reduced etching times and complete dissolution of the sacrificial layer, i.e., fewer residues. SU-8 devices released with the AZ 15nXT as sacrificial layer behave consistently compared to the devices which are released by other methods [18,19,31,39].…”

Section: Resultsmentioning

confidence: 78%

Sacrificial Layer Technique for Releasing Metallized Multilayer SU-8 Devices

et al. 2018

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The low fabrication cost of SU-8-based devices has opened the fields of point-of-care devices (POC), µTAS and Lab-on-Chip technologies, which call for cheap and disposable devices. Often this translates to free-standing, suspended devices and a reusable carrier wafer. This necessitates a sacrificial layer to release the devices from the substrates. Both inorganic (metals and oxides) and organic materials (polymers) have been used as sacrificial materials, but they fall short for fabrication and releasing multilayer SU-8 devices. We propose photoresist AZ 15nXT (MicroChemicals GmbH, Ulm, Germany) to be used as a sacrificial layer. AZ 15nXT is stable during SU-8 processing, making it suitable for fabricating free-standing multilayer devices. We show two methods for cross-linking AZ 15nXT for stable sacrificial layers and three routes for sacrificial release of the multilayer SU-8 devices. We demonstrate the capability of our release processes by fabrication of a three-layer free-standing microfluidic electrospray ionization (ESI) chip and a free-standing multilayer device with electrodes in a microchannel.

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“…It provides a unified view of large-scale resources on the Internet in encyclopedia mode to realize an integrated search model of multiplatform online resources across the network. The literature [23] argues that the regional education big data platform should have context awareness, the full recording of natural interaction data, semantic aggregation of learning resources, and automatic content transcoding and format adaptation. The core modules such as subject knowledge base, cognitive model and inference engine established in the platform are used to analyze the interaction data of the learning process and personalize the learning resources and learning services.…”

Section: Introductionmentioning

confidence: 99%

Research on the innovation theory of college students’ ideological and political education under the background of big data

Lai

2023

Applied Mathematics and Nonlinear Sciences

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In order to improve the ideological and political education of college students, this paper constructs a cloud technology education platform based on big data analysis and collection technology. Firstly, the collaborative filtering algorithm is used to filter and collect student information; secondly, the Pearson correlation formula is used for pre-processing, MAE (mean absolute error value) is used as an evaluation index; and finally, the data is combined with FCM (fuzzy C-mean) algorithm to filter and analyze as needed. Its application performance is examined to verify the rationality and practicality of the construction of the cloud technology education platform. The analysis results show that, compared with the three-tier neural network education platform and MOOC (Massive Open Online Course) education platform, the educational resource transmission time of the cloud technology education platform constructed in this paper is reduced by 1/3, and the load balancing deviation is reduced by 1/4. Among four different servers, the average error value of the platform constructed in this paper is as low as 0.35% and can reach as high as 0.65%. The detection rate reaches 97.64%, the false alarm rate is only 2.93%, and the leakage rate is only 1.13%. It can be seen that the cloud technology education platform constructed in this paper can improve the utilization rate of educational resources and realize the sharing of educational resources.

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Thiol–ene micropillar array electrospray ionization platform for zeptomole level bioanalysis

Cited by 6 publications

References 25 publications

Thiol–Ene Based Polymers as Versatile Materials for Microfluidic Devices for Life Sciences Applications

Thiol–Ene Based Polymers as Versatile Materials for Microfluidic Devices for Life Sciences Applications

Sacrificial Layer Technique for Releasing Metallized Multilayer SU-8 Devices

Research on the innovation theory of college students’ ideological and political education under the background of big data

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