Abstract:Background
Toxic gliadin peptide damages enterocytes in celiac disease by causing oxidative stress. Thiols are organic compounds that defend against oxidative stress. This study aimed to investigate the changes in thiol–disulfide homeostasis in children with celiac disease.
Methods
The study included patients with celiac disease, children diagnosed with functional gastrointestinal disorders, and healthy children. Patients’ serum native and total thiol–disulfide amounts, disulfide/total thiol percentage ratios,… Show more
“…Total thiols (including nonprotein and protein thiols) protect the body from oxidative stress. (28) In addition, resveratrol shielded GST activity from copper and iron-induced reduction, and the flies' response to oxidative stress and heavy metal detoxification was activated. This finding led us to believe that resveratrol's protective mechanism against copper and iron-induced toxicity is improved further by the enhancement of thiol-containing proteins other than the GSH molecule alone.…”
BackgroundCopper (Cu) and iron (Fe) are essential trace elements that when in excess are capable of causing cytotoxic effects leading to lipid peroxidation and promoting oxidative stress. Resveratrol (RES) is a natural polyphenol with antioxidant and anti-inflammatory properties. This study was carried out to evaluate the protective role of RES in Fe and Cu sulphate-induced oxidative stress in Drosophila melanogaster.
MethodsAdult wild type flies were fed Cu2+ and Fe2+ (1 mM each) and/or RES (30 and 60 mg/kg diet) for 7 days. Survival, negative geotaxis and emergence rate were evaluated by daily recording of fruit fly mortality and final analysis. Fruit flies were anaesthetized using CO2 gas, homogenized and centrifuged at 4,000 rpm for 10 minutes at 4 °C. Aliquots of the supernatants were used for the estimation of biochemical markers using spectrophotometry.
ResultsFruit flies co-treated with FeSO4 + CuSO4 (1 mM each) + RES (30 and 60 mg/Kg) significantly elevated H2O2, NO, lipid peroxidation, acetylcholinesterase as well as GSH, GST, catalase and total thiols (p<0.05) compared with the Cu2+ + Fe2+ (1mM each) treated group. Flies co-treated with FeSO4 + CuSO4 (1mM each) + RES (30 and 60mg/Kg) also had significantly improved (p< 0.05) eclosion and climbing rates compared with the Cu2+ + Fe2+ (1mM each) treated group.
ConclusionThis study demonstrated that RES reduced Cu2+ and Fe2+-induced radical generation in D. melanogaster and improved the antioxidant buffering capability of the flies. Therefore, RES could be used in management of disorders involving oxidative stress.
“…Total thiols (including nonprotein and protein thiols) protect the body from oxidative stress. (28) In addition, resveratrol shielded GST activity from copper and iron-induced reduction, and the flies' response to oxidative stress and heavy metal detoxification was activated. This finding led us to believe that resveratrol's protective mechanism against copper and iron-induced toxicity is improved further by the enhancement of thiol-containing proteins other than the GSH molecule alone.…”
BackgroundCopper (Cu) and iron (Fe) are essential trace elements that when in excess are capable of causing cytotoxic effects leading to lipid peroxidation and promoting oxidative stress. Resveratrol (RES) is a natural polyphenol with antioxidant and anti-inflammatory properties. This study was carried out to evaluate the protective role of RES in Fe and Cu sulphate-induced oxidative stress in Drosophila melanogaster.
MethodsAdult wild type flies were fed Cu2+ and Fe2+ (1 mM each) and/or RES (30 and 60 mg/kg diet) for 7 days. Survival, negative geotaxis and emergence rate were evaluated by daily recording of fruit fly mortality and final analysis. Fruit flies were anaesthetized using CO2 gas, homogenized and centrifuged at 4,000 rpm for 10 minutes at 4 °C. Aliquots of the supernatants were used for the estimation of biochemical markers using spectrophotometry.
ResultsFruit flies co-treated with FeSO4 + CuSO4 (1 mM each) + RES (30 and 60 mg/Kg) significantly elevated H2O2, NO, lipid peroxidation, acetylcholinesterase as well as GSH, GST, catalase and total thiols (p<0.05) compared with the Cu2+ + Fe2+ (1mM each) treated group. Flies co-treated with FeSO4 + CuSO4 (1mM each) + RES (30 and 60mg/Kg) also had significantly improved (p< 0.05) eclosion and climbing rates compared with the Cu2+ + Fe2+ (1mM each) treated group.
ConclusionThis study demonstrated that RES reduced Cu2+ and Fe2+-induced radical generation in D. melanogaster and improved the antioxidant buffering capability of the flies. Therefore, RES could be used in management of disorders involving oxidative stress.
“…Children with CeD and FAP were diagnosed based on the European Society for Pediatric Gastroenterology Hepatology and Nutrition (ESPGHAN) 2012 criteria, modified Marsh (Oberhuber) classification and Rome 4 diagnostic criteria (1,9).…”
Section: Study Outline and Sample Collectionmentioning
Background: Blastocystis has been associated with various symptoms of the gastrointestinal tract. We aimed to investigate the prevalence of Blastocystis in children with celiac disease (CeD) or functional abdominal pain (FAP) and to evaluate its subtypes (STs) with respect to demographic, socioeconomic and epidemiological factors.
Methods: Overall, 161 fecal samples were collected from healthy children and patients with FAP or CeD in Hitit University Erol Olçok Research and Training Hospital, Corum, Turkey between 2016-2018. Samples were examined using both native-Lugol (NL) and trichrome-stained (TS) smears, and further analyses by PCR and Sanger sequencing were performed. A standard questionnaire was applied to obtain demographic, socioeconomic, epidemiological data.
Results: Blastocystis was found in 10.6% of the total study population. Neither bacteria nor any other parasites were found, except for one Giardia (0.6%) in the CeD group. The presence/absence of the parasite was not found to be associated with demographic, socioeconomic and epidemiological factors. Blastocysis was detected in 11.5% (6/52) of the CeD, 7.7% (4/52) of the FAP, and 12.3% (7/57) of the healthy group. Diagnostic methods were similar in terms of Blastocystis detection (P= 0.671), and there was fair agreement between the NL, TS and PCR (Fleiss' Kappa=0.847, P=0.001). ST2 (42.8%) and ST3 (35.7%) were the predominant STs followed by ST1 (21.4%).
Conclusion: We observed no difference between study groups in terms of Blastocystis prevalence. ST1, ST2 and ST3 subtypes were detected. Blastocystis prevalence and STs were not related to any of the demographic, socioeconomic and epidemiological factors.
“…Many studies address the role of dynamic TDH in a variety of diseases in different age groups. There is growing evidence that TDH plays a role in various medical conditions such as celiac disease, diabetes mellitus, rheumatoid arthritis, and neurofibromatosis 8 , 16 - 19 as well as in various mental disorders such as schizophrenia, bipolar disorder (BD)-manic episode, obsessive-compulsive disorder (OCD), and autism spectrum disorder (ASD). 20 - 23 The aim of this study is to contribute to the etiopathogenesis and treatment of MDD in adolescents by investigating a novel marker for OS, TDH, and determining the relationship between TDH and clinical variables such as depression severity and irritability.…”
Background:
Thiol-disulfide homeostasis (TDH), one of the most important antioxidants, is involved in the non-enzymatic removal of reactive oxygen molecules in the body and is one of the many methods to measure the level of oxidative stress (OS). In the present study, TDH is investigated in adolescent depression, and its relationship to clinical variables is examined.
Methods:
Thirty-two (50.0%) patients diagnosed with major depressive disorder (MDD) and without psychotropic drug use and 32 (50.0%) healthy controls were included in the present study. The subjects MDD and control groups were between 13 and 18 years old. Participants completed the DSM-5 Level-2 scales for depression and irritability. A colorimetric method proposed by Erel and Neselioglu was used to analyze the TDH parameters of serum samples.
Results:
Biochemical analyses of samples from the MDD and control groups showed significant differences between the groups in native thiol (SH) levels (
P
= .002), disulfide (SS) levels (
P
= .021), disulfide/total thiol (SS/ToSH) (
P
= .009), and disulfide/native thiol (SS/SH) (
P
= .003) levels. Analysis of receiver operating characteristic showed that the area under the curve values with “acceptable discrimination potential” for the TDH parameters were significantly able to discriminate individuals with MDD from healthy controls.
Conclusion:
Thiol-disulfide homeostasis, one of the OS parameters, was found to be impaired in adolescents with depression. Our results suggest that TDH may contribute to the etiopathogenesis of adolescent MDD and that TDH may be a novel approach to assess OS in adolescent depression.
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