Detection of Blastocystis Subtypes in Children with Functional Abdominal Pain and Celiac Disease in Çorum, Turkey

Güreser, Ayşe Semra; Comba, Atakan; Karasartova, Djursun; Koşar, Nezahat; Keskin, Adem; Stensvold, Christen Rune; Taylan-Özkan, Ayşegül

doi:10.18502/ijpa.v17i3.10619

Cited by 6 publications

(4 citation statements)

References 34 publications

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“…Blastocystis hominis is protozoa commonly found in the gastrointestinal tract of humans and a wide variety of animals can be acquired via the fecal-oral route through contaminated water and food or can be by exposure to animals. (Güreser et al, 2022). In this study, we found that opportunistic intestinal protozoan infection was more commonly isolated from boys than girls, this is probably because boys are more active in playing outside the orphanage and are less concerned about their hygiene.…”

Section: Resultsmentioning

confidence: 56%

Detection of Opportunistic Intestinal Protozoans in The Feces of Orphanage Children in Pekanbaru

2022

Nusantara Science and Technology Proceedings

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Opportunistic intestinal protozoa are intestinal protozoa that can infect humans and cause clinical symptoms depending on the patient's immune status. In immunocompromised patients, infection by opportunistic intestinal protozoa can cause symptoms of diarrhea; mild to severe diarrhea. In addition to immunocompromised patients, these opportunistic intestinal protozoa can also be found in children with poor personal hygiene and environmental sanitation. This research was a descriptive study that aims to determine the incidence of opportunistic intestinal protozoa infection in orphanage children in Pekanbaru, which was conducted from August until October 2022. The result of microscopic examination with acid-fast staining were 70 samples of children's feces from 8 of the orphanage found 41,4% were positive for opportunistic intestinal protozoans; consisting of 11.4% Cryptosporidium sp. infection, Blastocystis hominis as much as 21.4%, Cyclospora sp as much as 1.4%. and mixed infections of Blastocystis hominis and Cryptosporidium sp. as much as 7.2%. The prevalence of opportunistic intestinal protozoa found in the feces of orphanage children in Pekanbaru is high, but to our knowledge, these intestinal protozoans will not cause definite symptoms as long as the individual's immune status is good.

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Section: Resultsmentioning

confidence: 56%

Detection of Opportunistic Intestinal Protozoans in The Feces of Orphanage Children in Pekanbaru

2022

Nusantara Science and Technology Proceedings

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“…In a study evaluating Blastocystis subtypes in children with functional abdominal pain and celiac disease in Türkiye, there was no difference in Blastocystis prevalence between study groups. ST2, ST3, and ST1 were the most common subtypes, respectively (Güreser et al 2022). In a study that determined the prevalence and subtypes distribution of Blastocystis among school-aged children in Thailand, the most common subtypes were ST3, followed by ST1 and ST2 (Abu et al 2022).…”

Section: Discussionmentioning

confidence: 99%

Investigation of intestinal parasites by conventional and molecular methods in children with gastrointestinal system complaints

Özkan-Ahmetoğlu¹,

Demirel

Taşar³

et al. 2023

Parasitol Res

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Background and aims:Intestinal parasitic infections are a global health problem that causes morbidity and mortality, especially in children living in rural areas. In this study, stool samples of pediatric patients with gastrointestinal complaints were examined by conventional and molecular methods to determine the prevalence of intestinal parasites. Methods:A total of 100 pediatric patients with gastrointestinal complaints and 50 healthy children were included in the study. Stool samples were collected from each child and examined by direct microscopic examination (native-Lugol method), formol-ethyl acetate concentration technique, Kinyoun's acid-fast staining, and Wheatley trichrome staining methods. Real-time PCR was used for the detection of Blastocystis sp. and D. fragilisin the stool samples. Sanger sequencing was used to identify Blastocystissp. subtypes. Results:One or more intestinal parasites were found in 12% (n=100) of the patient group and 1% (n=50) of the control group using conventional techniques. By using real-time PCR, Blastocystis sp. was discovered in 14% (14/100) of the patient group and 4% (4/50) of the control group. There was no signi cant difference in the frequency of Blastocystis sp. between the two groups. The most prevalent Blastocystissubtype was ST1 and the most frequent allele was a2 among the samples successfully ampli ed and sequenced. D. fragilis was detected in 17% (17/100) of the patient group and 8% (4/50) of the control group by real-time PCR. The prevalence of D. fragiliswas not signi cantly different between the patient and control groups, as well. Conclusions:Blastocystis sp. and D. fragilis were found in high prevalence in pediatric patients with gastrointestinal complaints in this study. Although the role of these protists as a pathogen in humans is still controversial, it is supposed to the presence of the parasites are associated with gastrointestinal disorders such as diarrhea, abdominal pain, nausea, and vomiting. More case-control studies are needed to understand the pathogenic or commensal role of these parasites on the intestinal microbiota, especially in both patients with gastrointestinal disorders and healthy individuals.

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“…The infection is associated with at least 14 STs (ST1‐ST10, ST12, ST14, ST16, and ST24) in humans and animals, suggesting a high likelihood of zoonotic transmission. Additionally, more than 95% of Blastocystis cases in humans are thought to be caused by ST1–ST4 (Güreser et al., 2022; Maloney et al., 2022). Some STs are highly host‐specific, for example, ST5 commonly infects pigs while ST6 and ST7 predominate in birds.…”

Section: Introductionmentioning

confidence: 99%

First molecular characterization of Blastocystis subtypes from domestic animals (sheep and cattle) and their animal‐keepers in Ilam, western Iran: A zoonotic concern

Shams,

Bahrami,

Mousivand

et al. 2024

J Eukaryotic Microbiology

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A total of 360 fecal samples were randomly collected from 150 cattle, 150 sheep, and 60 humans (30 people with close animal contact and 30 individuals without close animal contact) at 10 farms in Ilam, western Iran from June 2022 to August 2023. All samples were directly examined for Blastocystis by zinc sulfate flotation, followed by microscopic observation. Positive samples were further subtyped using conventional PCR and sequencing methods. A mean prevalence of 5.3% (16/300) was estimated for Blastocystis infection among examined animals, with 6% and 4.7% for cattle and sheep, respectively. Among the people who had close and non‐close animal contact, 16.7% (5/30) and 3.3% (1/30) were infected with Blastocystis, respectively (p < 0.05). All 22 positive samples were successfully sequenced at the SSU rRNA locus. Accordingly, Blastocystis isolates infecting domestic animals in Ilam belonged to the four STs (ST1‐ST3, and ST10). Of the 16 animal isolates, nine sequences (four ST10, three ST3, and two ST1) were related to cattle, and seven sequences (three ST10, two ST3, and two ST2) were isolated from sheep. Among the six human isolates, ST3 was the most predominant ST, followed by STs 1, 2, 6, and 7 (one case each). Of note, ST1‐ST3 were isolated in various farms both from animals and their breeders, which indicates the possible circulation of these STs between animal and human populations.

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Detection of Blastocystis Subtypes in Children with Functional Abdominal Pain and Celiac Disease in Çorum, Turkey

Cited by 6 publications

References 34 publications

Detection of Opportunistic Intestinal Protozoans in The Feces of Orphanage Children in Pekanbaru

Detection of Opportunistic Intestinal Protozoans in The Feces of Orphanage Children in Pekanbaru

Investigation of intestinal parasites by conventional and molecular methods in children with gastrointestinal system complaints

First molecular characterization of Blastocystis subtypes from domestic animals (sheep and cattle) and their animal‐keepers in Ilam, western Iran: A zoonotic concern

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