Thiol/disulfide homeostasis as a marker of oxidative stress in rosacea: a controlled spectrophotometric study

Şener, Sertaç; Akbaş, Ayşe; Kılınç, Fadime; Baran, Pervin; Erel, Özcan; Aktaş, Akın

doi:10.1080/15569527.2018.1517124

Cited by 38 publications

(38 citation statements)

References 29 publications

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“…40,41 The observation that NaF depleted total thiol level further implies oxidative stress and impairment of the normal physiology of the flies since a decrease in the level of thiols has been noted in various medical disorders. 42 In the co-exposure paradigm, resveratrol caused a complete restoration of total thiol level depicting its antioxidative property. This is in accordance with Abolaji et al .…”

Section: Discussionmentioning

confidence: 99%

Protective role of resveratrol, a natural polyphenol, in sodium fluoride-induced toxicity inDrosophila melanogaster

Abolaji

Ajala

Adigun

et al. 2019

Exp Biol Med (Maywood)

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Sodium fluoride (NaF) is used in water fluoridation and dental products such as mouth rinses and toothpastes. Resveratrol is a natural polyphenol with antioxidant and anti-inflammatory properties. The present study was carried out to evaluate the toxicity of NaF and the protective role of resveratrol in Drosophila melanogaster. For longevity assay, Harwich strain of D. melanogaster was treated with NaF (0, 10, 30, 50, 70 and 90 mg/kg diet) throughout the lifespan and daily mortality recorded. Then, flies were again treated with similar doses of NaF for seven days to evaluate survival rate and oxidative stress markers. Thereafter, 60 mg resveratrol/kg diet was selected to determine its ameliorative role in NaF (70 mg/kg)-induced toxicity in flies: Group A (control), Group B (60 mg resveratrol/kg diet), Group C (70 mg NaF/kg diet), and Group D (resveratrol, 60 mg/kg diet) + NaF, 70 mg/kg diet). Thereafter, Glutathione-S-transferase (GST), catalase and acetylcholinesterase (AchE) activities, as well as total thiol (T-SH), nitrites/nitrates and hydrogen peroxide (H2O2) levels were determined. The results showed that resveratrol prevented NaF-induced elevation of H2O2 and nitrites/nitrates levels, as well as catalase activity. In addition, resveratrol restored NaF-induced inhibition of GST and AChE activities and depletion of T-SH content ( P < 0.05). Conclusively, resveratrol offered protective benefit against NaF-mediated toxicity in flies due to its antioxidant and anti-inflammatory properties. Impact statement D. melanogaster was used to understand the impact of NaF on lifespan and emergence rate as well as the rescue role of resveratrol. These parameters are difficult to carry out in previously used models such as rodents. This further enforces in part, the suitability of D. melanogaster in studying NaF-induced toxicity and the therapeutic effects of drugs. Additionally, we found that resveratrol rescued D. melanogaster from oxidative stress-induced by sodium fluoride (NaF) administration. This study is of public health significance as it indicated that the consumption of fruits rich in resveratrol such as grapes may offer protective role against inadvertent exposure to NaF and related chemicals.

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Section: Discussionmentioning

confidence: 99%

Protective role of resveratrol, a natural polyphenol, in sodium fluoride-induced toxicity inDrosophila melanogaster

Abolaji

Ajala

Adigun

et al. 2019

Exp Biol Med (Maywood)

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“…Natural immune system disorder, chronic inflammatory responses, microorganisms, neurovascular changes, ultraviolet radiation, and other environmental triggers, food and chemicals taken, alone or in combination, may be responsible for rosacea 5,6 . Recent studies have shown the significant role of oxidative stress on rosacea pathophysiology, and oxidative stress has been argued to be associated with vascular changes, inflammation and oxidative tissue damage in rosacea 3,7 …”

Section: Introductionmentioning

confidence: 99%

Serum bilirubin and uric acid antioxidant levels in rosacea patients

Türkmen

2020

J of Cosmetic Dermatology

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Background Rosacea is a common chronic inflammatory skin disease that the pathogenesis is not fully understood. Although the significant role of oxidative stress in rosacea pathophysiology has been shown in recent studies, there is no study addressing the potential roles of bilirubin and uric acid (UA) in rosacea. Aim The purpose of this study was to assign serum bilirubin and UA antioxidant levels in rosacea patients. Methods Eighty‐seven rosacea patients and 81 healthy controls (HCs) similar age and gender were included in the study. From all study participants, blood samples were taken and the values of total bilirubin (Tbil), direct bilirubin (Dbil), indirect bilirubin (Ibil), and UA were analyzed in the laboratory of biochemistry. Results The type of rosacea was erythematotelangiectatic in 51.7% of the patients, papulopustular in 43.7%, and phymatous in 4.6%. In rosacea group serum, Tbil, Dbil, Ibil, and UA values were found to be significantly lower than in the HCs. Male rosacea patients were found to have lower Tbil, Dbil, Ibil, and UA levels when compared with the males in the HCs. There was also the same significant difference in female patients. Conclusion Main finding of our study was that when compared with the control group, serum bilirubin and UA levels were significantly lower in rosacea patients. These levels sustain the hypothesis that antioxidant status and oxidative stress are important in the pathogenesis of rosacea.

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“…In more than 40% of rosacea subjects, there is a genetic defect in the production of glutathione transferase. Furthermore, in rosacea patients an altered ratio GSSG/GSH, an accurate indicator of the oxidative status of the cell, is significantly higher in comparison with healthy controls 29 . For these reasons, therapeutic strategies with the aim to increase GSH intracellular levels could be, at least theoretically, an interesting tool counter fighting the pathogenetic role of oxidative stress in rosacea.…”

Section: Discussionmentioning

confidence: 99%

Efficacy and tolerability of a cream containing modified glutathione (GSH‐C4), beta‐Glycyrrhetic, and azelaic acids in mild‐to‐moderate rosacea: A pilot, assessor‐blinded, VISIA and ANTERA 3‐D analysis, two‐center study (The “Rosazel” Trial)

Dall’Oglio

Puviani

Milani

et al. 2020

J of Cosmetic Dermatology

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Background Rosacea is a very common, chronic inflammatory disease characterized by flushing, erythema and inflammatory lesions. Increased oxidative stress plays a relevant pathogenetic role in Rosacea. Intracellular Glutathione (GSH) is the main scavenger protective mechanism against increased oxidative stress. An altered GSH metabolism in Rosacea has been described. GSH‐C4 is a modified GSH molecule characterized by a better intracellular bioavailability and longer half‐life. A daily cream (E‐AR) containing GSH‐C4 (0.1%) with beta‐Glycyrrhetic (0.5%) and azelaic acids (10%), with an SPF of 30, is available. Aim In a pilot, prospective, two‐center, assessor‐blinded study we evaluate the efficacy and the tolerability of E‐AR cream in subjects with mild to moderate Rosacea treated for 8 weeks. Patients and Methods The main outcomes were the Investigator Global Assessment (IGA) 7‐point score (from 0, completely clear; to 6, severe) and the clinical and instrumental erythema severity score (ESS) (from 0 to 4) evaluated in a blinded fashion (randomly coded photographs) at baseline, after 4 (only clinical) and 8 weeks (clinical and instrumental). VISIA evaluation for erythema and lesion counts and ANTERA 3D analysis for skin haemoglobin concentration (a parameter associated with inflammation) were also performed at the same time points. Analysis of primary outcomes was performed on an intention‐to‐treat basis. Tolerability was evaluated at week 4 and 8 recording spontaneously reported side effects. Results Thirty subjects (22 women and 8 men; mean age 38 years) were enrolled after their written informed consent. Twenty‐six (87%) subjects completed the study phases. Four subjects stopped prematurely the trial due to low skin tolerability (n=3) or lost to follow‐up (n=1). At baseline, mean (SD) IGA score was 2.6 (0.9). At week 4, IGA score decreased (NS) to 2.3 (1.2). IGA score decreased significantly (p=0.0001) at week 8 to 1.2 (1) (mean difference 1.3; 95% CI of the difference from 0.9 to 1.7) in comparison with the baseline. The inflammatory mean (SD) lesion count, evaluated clinically, were 5.1(2.5) at baseline, 2.8 (1.9) at week 4, and 1.9 (1.7) at week 8 (P=0.0001; ANOVA Test), representing a 63% reduction. This reduction was confirmed by inflammatory lesions count performed on VISIA pictures (from 4.5 at baseline to 1.7 lesions at week 8). Similar evolution was observed for the clinical and instrumental ESS with a reduction of 56% (clinical) and 48% (VISIA), respectively, at week 8 in comparison with the baseline. ANTERA 3D photographs confirmed the positive evolution observed clinically with a significant reduction (‐24%) in hemoglobin content: from 1.88 at baseline to 1.44 at week 8. Conclusion This new GSH‐C4, beta‐glycyrrethic and azelaic acids cream has shown to be efficacious in mild to moderate rosacea subjects. Local tolerability is in line with other anti‐rosacea treatments.

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Thiol/disulfide homeostasis as a marker of oxidative stress in rosacea: a controlled spectrophotometric study

Abstract: In rosacea patients, the thiol/disulfide balance was observed to shift towards disulfides, which could be considered an indicator of oxidative stress in rosacea.

Cited by 38 publications

References 29 publications

Protective role of resveratrol, a natural polyphenol, in sodium fluoride-induced toxicity inDrosophila melanogaster

Protective role of resveratrol, a natural polyphenol, in sodium fluoride-induced toxicity inDrosophila melanogaster

Serum bilirubin and uric acid antioxidant levels in rosacea patients

Efficacy and tolerability of a cream containing modified glutathione (GSH‐C4), beta‐Glycyrrhetic, and azelaic acids in mild‐to‐moderate rosacea: A pilot, assessor‐blinded, VISIA and ANTERA 3‐D analysis, two‐center study (The “Rosazel” Trial)

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