Thiobacillus ferrooxidans cytochrome c-552: purification and some of its molecular features

Sato, Akihiko; Fukumori, Yoshihiro; Yano, Takahiro; Kai, Masahiro; Yamanaka, T.

doi:10.1016/s0005-2728(89)80221-x

Cited by 36 publications

(31 citation statements)

References 16 publications

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“…Several c-type cytochromes in membrane-bound and soluble form have been purified from aerobically grown A. ferrooxidans cells (4,11,16,20,35,40,41,43). The present study is the first to purify a cytochrome from anaerobically grown A. ferrooxidans cells.…”

Section: Resultsmentioning

confidence: 81%

“…The present study is the first to purify a cytochrome from anaerobically grown A. ferrooxidans cells. The purified soluble protein, which has a molecular mass of 28 kDa, differs in size from the previously described cytochromes (4,11,16,20,35,40,41,43). Moreover, the sequence of N-terminal 30 amino acids of the purified protein was preliminarily determined (data not shown) and found to be dissimilar to the known sequences of either the soluble or membrane-bound cytochromes (11,16,20,43).…”

Section: Resultsmentioning

confidence: 87%

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Anaerobic Respiration Using Fe ³⁺ , S ⁰ , and H ₂ in the Chemolithoautotrophic Bacterium Acidithiobacillus ferrooxidans

et al. 2002

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The chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans has been known as an aerobe that respires on iron and sulfur. Here we show that the bacterium could chemolithoautotrophically grow not only on H 2 /O 2 under aerobic conditions but also on H 2 /Fe 3؉ , H 2 /S 0 , or S 0 /Fe 3؉ under anaerobic conditions. Anaerobic respiration using Fe 3؉ or S 0 as an electron acceptor and H 2 or S 0 as an electron donor serves as a primary energy source of the bacterium. Anaerobic respiration based on reduction of Fe 3؉ induced the bacterium to synthesize significant amounts of a c-type cytochrome that was purified as an acid-stable and soluble 28-kDa monomer. The purified cytochrome in the oxidized form was reduced in the presence of the crude extract, and the reduced cytochrome was reoxidized by Fe 3؉ . Respiration based on reduction of Fe 3؉ coupled to oxidation of a c-type cytochrome may be involved in the primary mechanism of energy production in the bacterium on anaerobic iron respiration.Acidithiobacillus ferrooxidans is generally accepted to be an aerobic chemolithoautotroph that derives energy for growth from oxidative respiration involving the oxidation of ferrous iron or various sulfur compounds. Brock and Gustafson reported that the bacterium reduces Fe 3ϩ in the presence of S 0 (10). However, the reduction was not recognized as respiratory reactions since iron reduction did not permit growth of the bacterium (39). Therefore, it was accepted that coupling reduction of Fe 3ϩ to oxidation of S 0 was one of the steps in the sulfur metabolism by the bacterium (39). Pronk et al. (32,33) and Das et al. (12) showed that the bacterium grew on the oxidation of S 0 by Fe 3ϩ under oxygen-limited conditions. Although these findings raised the possibility that A. ferrooxidans might be able to grow under anaerobic conditions, the aforementioned enzymatic activity was not accompanied by growth (39), and it is still unclear whether Fe 3ϩ serves as an electron acceptor for anaerobic respiration. On the other hand, the bacterium would grow on hydrogen under aerobic conditions (14). In that case, H 2 served as the electron donor enabling an oxidative respiratory chain to derive energy for chemolithoautotrophic growth.On the other hand, in many facultative heterotrophs in both Archaea and Bacteria, anaerobic respiration involving reduction of Fe 3ϩ or S 0 is typically coupled to the oxidation of H 2 (25,26,30,38,42). One of the principle roles of such respiration would have been to support energy for chemolithoautotrophy (34), a type of autotrophy that typically served as the growth mode of such facultative heterotrophs in Archaea and Bacteria as hyperthermophilic archaebacteria, sulfur-reducing bacteria, and primitive fermentative bacteria (18, 24, 36-38, 42, 44). However, little is known about the role played by anaerobic respiration involving Fe 3ϩ or S 0 reduction in the growth of typical, known chemolithoautotrophic bacteria, which include a variety of sulfur, iron, ammonia, and nitrite oxidizers. We have found tha...

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Section: Resultsmentioning

confidence: 81%

Section: Resultsmentioning

confidence: 87%

Anaerobic Respiration Using Fe ³⁺ , S ⁰ , and H ₂ in the Chemolithoautotrophic Bacterium Acidithiobacillus ferrooxidans

et al. 2002

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“…A number of laboratories have described redox-active proteins expressed by At. ferrooxidans that might participate in the iron respiratory chain (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13). Perhaps the most generally accepted model for the iron respiratory chain of At.…”

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confidence: 99%

The Multicenter Aerobic Iron Respiratory Chain of Acidithiobacillus ferrooxidans Functions as an Ensemble with a Single Macroscopic Rate Constant

Painter

Ban

et al. 2015

Journal of Biological Chemistry

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Background:The order of electron transfer in the respiratory chain of Acidithiobacillus ferrooxidans is unknown. Results:The multicenter respiratory chain functioned as an ensemble with a single macroscopic rate constant. Conclusion:The crowded respiratory proteins behaved as would a single protein with a common standard reduction potential of 650 mV. Significance: In situ spectroscopy constitutes a new and effective means to study cellular respiration.

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“…On the other hand, several diŠerent C-type cytochromes have been discovered in several strains of the bacterium. 9,12,[29][30][31][32][33][34][35] These results together, rus is present with C-type cytochrome in an apparent operon in strain ATCC 33020, and, multiple C-type cytochromes exist in some strains of the bacterium, suggested the possibility that some strains might also synthesize additional isoforms of rusticyanin. In this study, therefore, we isolated rus from six strains of A. ferrooxidans and identiˆed its copy number, sequence, and some of the physical characteristics of its gene product.…”

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confidence: 76%

“…[1][2][3] Aerobic respiration on iron is the principle metabolic activity of the chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans, which obtains all of its energy for CO2ˆxation and growth from oxidation of ferrous ions. [4][5][6][7][8][9][10] In that process, A. ferrooxidans uses rusticyanin as an electron transfer protein within its oxidative respiratory chain. 4,5,[10][11][12][13][14][15] This type I protein belongs to the copper protein superfamily the members of which are widely distributed in numerous organisms, 13,16) however, rusticyanin is the only member known to have both the high redox potential (＋680 mV) necessary for an electron carrier and acid stability at pH 2-3 where A. ferrooxidans thrives.…”

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confidence: 99%

Respiratory Isozyme, Two Types of Rusticyanin ofAcidithiobacillus ferrooxidans

Sasaki

Ida

Andõ

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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Thiobacillus ferrooxidans cytochrome c-552: purification and some of its molecular features

Cited by 36 publications

References 16 publications

Anaerobic Respiration Using Fe ³⁺ , S ⁰ , and H ₂ in the Chemolithoautotrophic Bacterium Acidithiobacillus ferrooxidans

Anaerobic Respiration Using Fe ³⁺ , S ⁰ , and H ₂ in the Chemolithoautotrophic Bacterium Acidithiobacillus ferrooxidans

The Multicenter Aerobic Iron Respiratory Chain of Acidithiobacillus ferrooxidans Functions as an Ensemble with a Single Macroscopic Rate Constant

Respiratory Isozyme, Two Types of Rusticyanin ofAcidithiobacillus ferrooxidans

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Thiobacillus ferrooxidans cytochrome c-552: purification and some of its molecular features

Cited by 36 publications

References 16 publications

Anaerobic Respiration Using Fe 3+ , S 0 , and H 2 in the Chemolithoautotrophic Bacterium Acidithiobacillus ferrooxidans

Anaerobic Respiration Using Fe 3+ , S 0 , and H 2 in the Chemolithoautotrophic Bacterium Acidithiobacillus ferrooxidans

The Multicenter Aerobic Iron Respiratory Chain of Acidithiobacillus ferrooxidans Functions as an Ensemble with a Single Macroscopic Rate Constant

Respiratory Isozyme, Two Types of Rusticyanin ofAcidithiobacillus ferrooxidans

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Anaerobic Respiration Using Fe ³⁺ , S ⁰ , and H ₂ in the Chemolithoautotrophic Bacterium Acidithiobacillus ferrooxidans

Anaerobic Respiration Using Fe ³⁺ , S ⁰ , and H ₂ in the Chemolithoautotrophic Bacterium Acidithiobacillus ferrooxidans