Respiratory Isozyme, Two Types of Rusticyanin of<i>Acidithiobacillus ferrooxidans</i>

Sasaki, Kazuhiro; Ida, Chigusa; Andõ, Akira; Matsumoto, Norio; Saiki, Hiroshi; Ohmura, Naoya

doi:10.1271/bbb.67.1039

Cited by 22 publications

(11 citation statements)

References 41 publications

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“…Rusticyanin is the A. ferrooxidans redox protein which has been the most extensively studied. Two types of rusticyanin were reported (Sasaki et al 2003). Rus A gene was common in previous reports.…”

Section: Discussionmentioning

confidence: 89%

Phylogenetic and genetic characterization of Acidithiobacillus strains isolated from different environments

Liu

Zhang

et al. 2014

World J Microbiol Biotechnol

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To study the phylogenetic relationships and genetic heterogeneity of 21 Acidithiobacillus strains isolated from different environments, we amplified and sequenced the 16S-23S rRNA gene intergenic spacers (ITS) of all these strains. These sequence data, combined with related sequences available from GenBank, were divided into six phylogenetic groups by 16S rRNA gene and by 16S-23S rRNA gene sequence analysis. The results of phylogenetic analysis were consistent with those obtained by repetitive element PCR and arbitrarily primed PCR. In this research, the Acidithiobacillus ferrooxidans (A. ferrooxidans) strains were always separated into two groups in phylogenetic and cluster analyses. Genotypic analyses of the genes rusA, rusB, hip and iro suggest that these two groups may have different biochemical mechanisms for oxidizing ferrous iron. Strains in one A. ferrooxidans group were detected with rusA gene that encodes rusticyanin A which plays a very important role in the iron respiratory chain. The second A. ferrooxidans group was found to contain rusB gene which encode a homologous protein (RusB). The data suggested that ITS-based phylogeny is an effective tool to elucidate the relationships of Acidithiobacillus and that a different iron oxidation pathway may exist in different A. ferrooxidans groups.

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Section: Discussionmentioning

confidence: 89%

Phylogenetic and genetic characterization of Acidithiobacillus strains isolated from different environments

Liu

Zhang

et al. 2014

World J Microbiol Biotechnol

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“…ferrooxidans (Group I strains) (Figs 2-4). Also, the failure to detect any strain that harboured copies of both rusA and rusB, coupled with the finding that one of these cultures contained both Group I and Group IV strains, implies that the results of Sasaki et al (2003) were erroneous and due to the use of mixed cultures. However, the existence of two rusB genes in strain JCM 3865 was confirmed.…”

Section: Discussionmentioning

confidence: 99%

“…1999; Sasaki et al, 2003;Bruscella et al, 2005). The upstream and downstream regions of the two rusticyanin genes are also completely different.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic and genetic variation among Fe(II)-oxidizing acidithiobacilli supports the view that these comprise multiple species with different ferrous iron oxidation pathways

et al. 2011

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Autotrophic acidophilic iron-and sulfur-oxidizing bacteria of the genus Acidithiobacillus constitute a heterogeneous taxon encompassing a high degree of diversity at the phylogenetic and genetic levels, though currently only two species are recognized (Acidithiobacillus ferrooxidans and Acidithiobacillus ferrivorans). One of the major functional disparities concerns the biochemical mechanisms of iron and sulfur oxidation, with discrepancies reported in the literature concerning the genes and proteins involved in these processes. These include two types of high-potential iron-sulfur proteins (HiPIPs): (i) Iro, which has been described as the iron oxidase; and (ii) Hip, which has been proposed to be involved in the electron transfer between sulfur compounds and oxygen. In addition, two rusticyanins have been described: (i) rusticyanin A, encoded by the rusA gene and belonging to the well-characterized rus operon, which plays a central role in the iron respiratory chain; and (ii) rusticyanin B, a protein to which no function has yet been ascribed. Data from a multilocus sequence analysis of 21 strains of Fe(II)-oxidizing acidithiobacilli obtained from public and private collections using five phylogenetic markers showed that these strains could be divided into four monophyletic groups. These divisions correlated not only with levels of genomic DNA hybridization and phenotypic differences among the strains, but also with the types of rusticyanin and HiPIPs that they harbour. Taken together, the data indicate that Fe(II)-oxidizing acidithiobacilli comprise at least four distinct taxa, all of which are able to oxidize both ferrous iron and sulfur, and suggest that different iron oxidation pathways have evolved in these closely related bacteria. INTRODUCTIONThe majority of obligately chemolithoautotrophic acidophilic bacteria that can oxidize ferrous iron [Fe(II)], sulfur and reduced inorganic sulfur compounds (RISCs) have for many years been considered a priori to be strains of the well-documented species Acidithiobacillus ferrooxidans (At. ferrooxidans). The genus Acidithiobacillus has generally been considered to be a member of the Gammaproteobacteria, though recent phylogenetic analysis suggests it arose after divergence from the Alphaproteobacteria but before the Betaproteobacteria/Gammaproteobacteria split (Williams et al., 2010). There have been numerous reports suggesting that Fe(II)-oxidizing acidithiobacilli are a heterogeneous collection of bacteria with sufficient genetic variability to warrant classification as more than one species (Harrison, 1982(Harrison, , 1984Novo et al., 1996;Amils et al., 1998;Selenska-Pobell et al., 1998;Paulino et al., 2001;Karavaiko et al., 2003;Mitchell et al., 2003;Bergamo et al., 2004; Akbar et al., 2005;Waltenbury et al., 2005;Peng et al., 2006;Ni et al., 2007Ni et al., , 2008a. For instance, a Abbreviations: BV, bootstrap value; HiPIP, high potential iron-sulfur protein; MLSA, multilocus sequence analysis; PP, posterior probability; RISC, reduced inorganic sulfur compound.3T...

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“…Plasmid DNA, pET9a carrying the rus gene encoding the cupredoxin rusticyanin from A. ferrooxidans , was kindly provided by Dr Kazuhiko Sasaki, Central Research Institute of Electric Power Industry, Chiba, Japan. Rusticyanin was isolated from Eschirichia coli BL21 (DE3) harbouring the rus gene as reported previously (Sasaki et al ., 2003) with some modifications. We used acetic acid buffer (pH 4.0) and CM‐Sepharose (Sigma) instead of β‐alanin buffer (pH 4.0) and TSK‐gel CM‐650 column.…”

Section: Methodsmentioning

confidence: 99%

Internalization of bacterial redox protein azurin in mammalian cells: entry domain and specificity

Yamada¹,

Fialho²,

Punj³

et al. 2005

Cellular Microbiology

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Respiratory Isozyme, Two Types of Rusticyanin ofAcidithiobacillus ferrooxidans

Cited by 22 publications

References 41 publications

Phylogenetic and genetic characterization of Acidithiobacillus strains isolated from different environments

Phylogenetic and genetic characterization of Acidithiobacillus strains isolated from different environments

Phylogenetic and genetic variation among Fe(II)-oxidizing acidithiobacilli supports the view that these comprise multiple species with different ferrous iron oxidation pathways

Internalization of bacterial redox protein azurin in mammalian cells: entry domain and specificity

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