Thin peptide hydrogel membranes suitable as scaffolds for engineering layered biostructures

Seow, Wei Yang; Kandasamy, Karthikeyan; Purnamawati, Kristy; Sun, William; Hauser, Charlotte

doi:10.1016/j.actbio.2019.02.001

Cited by 17 publications

(14 citation statements)

References 49 publications

(53 reference statements)

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“…For the disulfide-linked gelation process, the presence of H 2 O 2 not only facilitates the dimerization process but also produces stronger hydrogels. 28 In the present case, the presence of H 2 O 2 certainly expedites the dimerization of 11 and 12 as found from the HPLC analyses (Figure 1). To verify any effect on the gel strength due to the presence of H 2 O 2 , the rheological behaviors of the hydrogels (24 h matured samples) prepared by using H 2 O 2 are determined.…”

Section: ■ Results and Discussionsupporting

confidence: 67%

“…One such peptide is Ac-IVKC-NH 2 , which forms one of the strongest peptide based hydrogels (elastic modulus of ∼0.9 MPa) reported so far. 28 All these reports prompted us to explore the possibility of creating new smart soft materials from short peptides containing the "KC" sequence at the C-terminal. It is important to note that all these reported "KC" peptides bear a short hydrophobic sequence/group at the N-terminal.…”

Section: ■ Introductionmentioning

confidence: 99%

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Smart Thixotropic Hydrogels by Disulfide-Linked Short Peptides for Effective Three-Dimensional Cell Proliferation

et al. 2020

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Supramolecular assembly of short peptides is a crucial process and has shown numerous potential applications as biomaterials. In the present work, the hydrogelation process of short peptides containing C-terminal “Lys–Cys” (KC) residues have been studied in detail. The N-terminal capping is found to be essential for effective gelation. Out of 12 peptides we studied, two of them could form hydrogels efficiently: Ac-VVKC-NH2 and Ac-FFKC-NH2. In both cases, the monomer-to-dimer formation through disulfide linkages by Cys residues controls the aggregation process. Interestingly, the presence of H2O2 facilitated the dimerization and thereby reduced the gelation time but could not impart much effect on the mechanical properties of the gels. Detailed rheological study revealed that both hydrogels are thixotropic in nature. Moreover, they are responsive to glutathione (GSH) due to the presence of disulfide linkages. However, the hydrogel of Ac-FFKC-NH2 is found to be stronger and more effective for biological applications. The thixotropic nature as well as a model drug release study in response to varying GSH concentration indicates the possible use of the hydrogel as an injectable local drug delivery vehicle. The hydrogel of Ac-FFKC-NH2 is noncytotoxic in nature. Three-dimensional cell proliferation has been found to be more effective than 2D, as it mimics the in vivo situation more closely if not exactly. In the present study, we have shown that both differentiated RAW macrophages and undifferentiated THP-1 monocytes could proliferate significantly within the 3D matrix of the hydrogel, without depicting any apparent cytotoxicity. Thus, the hydrogel of Ac-FFKC-NH2 has potential for application in localized drug administration and as a supporting biomaterial to study basic phenomena involving cell behavior.

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Section: ■ Results and Discussionsupporting

confidence: 67%

Section: ■ Introductionmentioning

confidence: 99%

Smart Thixotropic Hydrogels by Disulfide-Linked Short Peptides for Effective Three-Dimensional Cell Proliferation

et al. 2020

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“…2019 [203] Silicone contact lenses based on TRIS-DMA-NVP-HEMA hydrogels L929 fibroblasts TRIS content increases oxygen permeability and decreases water content. No cytotoxicity.…”

Section: Human Corneal Stromal and Epithelial Cellsmentioning

confidence: 99%

Hydrogels as Emerging Materials for Cornea Wound Healing

Khosravimelal

Mobaraki

Eftekhari

et al. 2021

Small

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According to a World Health Organization (WHO) estimation, over 10 million patients suffer from visual impairments and vision loss annually due to corneal diseases or injuries. [6,8] The corneal response to injury involves a highly complicated cascade of events that comprises cell adhesion, migration, differentiation, proliferation, death, and extracellular matrix (ECM) remodeling mediated by immunomodulators and growth factors. [9,10] Conserving the corneal transparency is critical for visual perception. Therefore, any destruction that affects its integrity caused by diseases or trauma like bullous keratopathy, keratoconus, and scarring can lead to blurred eyesight and eventually blindness. [4,11,12] Among all the therapeutic modalities that contributed to corneal irreversible damages including drug and gene therapy and surgical techniques, the keratoplasty with a human donor has offered the best clinical outcome with an 80% rate of success. [2,11,13] However, despite a high success rate, increasing demands for donor tissue and graft rejection due to severe immune response of the hosts are considered as the major reasons for developing an alternative to this procedure. [6,11,13,14] Cell therapies in combination with 3D biocompatible scaffolds have the great potential to replace donor tissue for transplantation or improve wound healing. [15] The potential of cell therapy in corneal wound healing has previously been discussed. [16] 3D scaffolds can mimic the composition and structure of the cornea and

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“…It arises through non-covalent interactions between components, including electrostatic, hydrophobic, hydrogen bonding and π-π interactions [ 29 ]. Depending on the specific building blocks and the assembly conditions, various nano-structured morphologies, including fibrils, tubes, sheets, tapes, spheres, vesicles and hydrogel matrices, can be formed in vitro, allowing for distinctive functional possibilities [ 28 , 30 , 31 , 32 , 33 , 34 , 35 ], mainly in tissue engineering [ 36 , 37 , 38 , 39 ], regenerative medicine [ 40 ], cell culture [ 41 , 42 , 43 , 44 ], drug delivery [ 36 , 39 , 45 , 46 , 47 , 48 , 49 ], bio-imaging [ 50 , 51 ] and fabric functionalization [ 52 ]. Coatings based on self-assembled peptides, primarily for antibacterial purposes, have been recently reported [ 5 , 12 , 13 , 21 , 53 , 54 , 55 , 56 ].…”

Section: Introductionmentioning

confidence: 99%

Surface Modification by Nano-Structures Reduces Viable Bacterial Biofilm in Aerobic and Anaerobic Environments

Ya'ari

Halperin‐Sternfeld

Rosin

et al. 2020

IJMS

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Bacterial biofilm formation on wet surfaces represents a significant problem in medicine and environmental sciences. One of the strategies to prevent or eliminate surface adhesion of organisms is surface modification and coating. However, the current coating technologies possess several drawbacks, including limited durability, low biocompatibility and high cost. Here, we present a simple antibacterial modification of titanium, mica and glass surfaces using self-assembling nano-structures. We have designed two different nano-structure coatings composed of fluorinated phenylalanine via the drop-cast coating technique. We investigated and characterized the modified surfaces by scanning electron microscopy, X-ray diffraction and wettability analyses. Exploiting the antimicrobial property of the nano-structures, we successfully hindered the viability of Streptococcus mutans and Enterococcus faecalis on the coated surfaces in both aerobic and anaerobic conditions. Notably, we found lower bacteria adherence to the coated surfaces and a reduction of 86–99% in the total metabolic activity of the bacteria. Our results emphasize the interplay between self-assembly and antimicrobial activity of small self-assembling molecules, thus highlighting a new approach of biofilm control for implementation in biomedicine and other fields.

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Thin peptide hydrogel membranes suitable as scaffolds for engineering layered biostructures

Cited by 17 publications

References 49 publications

Smart Thixotropic Hydrogels by Disulfide-Linked Short Peptides for Effective Three-Dimensional Cell Proliferation

Smart Thixotropic Hydrogels by Disulfide-Linked Short Peptides for Effective Three-Dimensional Cell Proliferation

Hydrogels as Emerging Materials for Cornea Wound Healing

Surface Modification by Nano-Structures Reduces Viable Bacterial Biofilm in Aerobic and Anaerobic Environments

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