Thin-layer chromatography with supersonic jet fluorometric detection

Imasaka, Totaro; Tanaka, Katsunori; Ishibashi, Nobuhiko

doi:10.1021/ac00203a012

Cited by 8 publications

(1 citation statement)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a result, the excitation wavelength is usually fixed at a resonance wavelength, which should be known prior to the experiment. To overcome this problem, the sample spotted in line on a thin-layer chromatographic plate can be developed and fitted beneath the nozzle throat, in which the plate can be moved two-dimensionally [57]. The chemical species in silica gel is ablated by laser pulses and is entrained into a supersonic jet.…”

Section: A12 Hyphenated Techniquesmentioning

confidence: 99%

Critical assessment: Use of supersonic jet spectrometry for complex mixture analysis (IUPAC Technical Report)

Imasaka¹,

Moore²,

Vo‐Dinh³

2003

Pure and Applied Chemistry

View full text Add to dashboard Cite

Republication or reproduction of this report or its storage and/or dissemination by electronic means is permitted without theAbstract: When cooled to a temperature of a few K using supersonic jet expansion into a vacuum, a molecule exists in the lowest vibrational level of the ground electronic state and is isolated at collision-free conditions. The absorption or excitation/fluorescence spectrum is then greatly simplified, when transitions occur from this single vibrational level to a limited number of vibrational levels in the excited electronic state. This method, called supersonic jet spectrometry, is a powerful analytical technique because of its high selectivity, since the chemical species can be accurately identified and selectively quantified using the sharp spectral features even for large molecules. Supersonic jet spectrometry has distinct advantages over other low-temperature spectrometries, in that it can be combined with gas-phase separation and detection techniques such as chromatography or mass spectrometry. Therefore, this spectrometric technique can be used as a versatile analytical means, not only for basic research on pure substances, but also for practical trace analysis of chemical species in multicomponent samples (e.g., in biological monitoring or in environmental monitoring).

show abstract

Section: A12 Hyphenated Techniquesmentioning

confidence: 99%

Critical assessment: Use of supersonic jet spectrometry for complex mixture analysis (IUPAC Technical Report)

Imasaka¹,

Moore²,

Vo‐Dinh³

2003

Pure and Applied Chemistry

View full text Add to dashboard Cite

show abstract

Synthesis and Spectroscopic Characterization of New NIR Absorbing (2‐Thienyl)‐ and (4‐Dialkylaminoaryl)‐substituted Croconic Acid Dyes

Keil¹,

Hartmann

Reichardt

1993

Liebigs Ann. Chem.

View full text Add to dashboard Cite

By condensation of croconic acid (7) with 2‐(dialkylamino)‐thiophenes 6a–d and with 3‐(dialkylamino)phenols 9a–c the deeply colored, sparingly soluble croconic acid dyes (“croconines”) 8a–d and 10a–c, respectively, have been prepared. These croconines have been characterized by UV/Vis, IR, and 1H‐NMR spectrometry. They exhibit strong electronic absorption bands in the near infrared (NIR) region (λmax ≈ 750–850 nm; cf. Table 1).

show abstract

Thin‐layer chromatography/laser desorption of peptides followed by multiphoton ionization time‐of‐flight mass spectrometry

Krutchinsky¹,

Dolgin²,

Utsal³

et al. 1995

J. Mass Spectrom.

View full text Add to dashboard Cite

The location of the separated compounds of GlnTrp/GlyTyr and pentagastrinlgramicidin D peptide model mixtures on silica gel and cellulose thin-layer chromatography plates has been examined by laser desorption multiphoton ionization time-of-flight mass spectrometry. The multiphoton ionization mass spectra of neutrals desorbed by sequential scanning of the thin-layer chromatography plates versus the infrared laser spot allowed imaging of the distribution profile of the compounds. The method appeared to be promising for the analysis of thin-layer chromatograms.

show abstract

Thin-layer chromatography with supersonic jet fluorometric detection

Cited by 8 publications

References 34 publications

Critical assessment: Use of supersonic jet spectrometry for complex mixture analysis (IUPAC Technical Report)

Critical assessment: Use of supersonic jet spectrometry for complex mixture analysis (IUPAC Technical Report)

Synthesis and Spectroscopic Characterization of New NIR Absorbing (2‐Thienyl)‐ and (4‐Dialkylaminoaryl)‐substituted Croconic Acid Dyes

Thin‐layer chromatography/laser desorption of peptides followed by multiphoton ionization time‐of‐flight mass spectrometry

Contact Info

Product

Resources

About