Thin‐layer chromatography/laser desorption of peptides followed by multiphoton ionization time‐of‐flight mass spectrometry

Krutchinsky, Andrew N.; Dolgin, A. I.; Utsal, O. G.; Khodorkovski, A. M.

doi:10.1002/jms.1190300221

Cited by 12 publications

(5 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…21 Because of its sensitivity, selectivity, and potential to be quantitative, postionization has been applied to many different areas of research. A few examples of surface analysis of molecular species using postionization include peptides desorbed from thinlayer chromatography plates, 22 polycyclic aromatic hydrocarbons from meteoritic acid residues, 23 and imaging of patterned biomolecules and polymers deposited on various surfaces. [24][25][26] It was previously suggested that postionization mass spectrometry could be used to characterize molecular biosensors.…”

mentioning

confidence: 99%

“…Because of its sensitivity, selectivity, and potential to be quantitative, postionization has been applied to many different areas of research. A few examples of surface analysis of molecular species using postionization include peptides desorbed from thin-layer chromatography plates, polycyclic aromatic hydrocarbons from meteoritic acid residues, and imaging of patterned biomolecules and polymers deposited on various surfaces. − It was previously suggested that postionization mass spectrometry could be used to characterize molecular biosensors . However, mass spectrometric studies of host−guest chemistry at surfaces have been mainly confined to matrix-assisted laser desorption and fast-atom bombardment. − Both of these methods often require extensive sample treatments that may not be appropriate in certain applications.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Surface Mass Spectrometry of Biotinylated Self-Assembled Monolayers

et al. 1997

View full text Add to dashboard Cite

Biotin and biotinylated self-assembled monolayers (SAMs) on gold have been investigated using time-of-flight secondary ion mass spectrometry, direct laser desorption, laser desorption with 193 nm photoionization of ion- and laser-desorbed species, and laser desorption with vacuum ultraviolet (VUV, 118 nm) photoionization. Our results indicate that direct laser desorption and laser desorption combined with 193 nm multiphoton ionization can detect a chromophoric molecule like biotin that is covalently bound to a SAM. However, secondary ion mass spectra were dominated by fragmentation, and ion desorption/193 nm photoionization detected no species related to biotin. The dominant features of the laser desorption/VUV mass spectra were neat and Au-complexed dimers of intact and fragmented biotinylated SAM molecules. Multiphoton and single-photon ionization of laser-desorbed neutrals from biotinylated SAMs both led to the production of ions useful for chemical analysis of the monolayer. Multiphoton ionization with ultraviolet radiation was experimentally less challenging but required a chromophore for ionization and resulted in significant fragmentation of the adsorbate. Single-photon ionization with VUV radiation was experimentally more challenging but did not require a chromophore and led to less fragmentation. X-ray photoelectron spectra indicated that the biotinylated SAM formed a disordered, 40-60 Å thick monolayer on Au. Additionally, projection photolithography with a Schwarzschild microscope was used to pattern the biotinylated SAM surface and laser desorption/photoionization was used to detect biotinylated adsorbates from the ∼10 μm sized pattern.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

Surface Mass Spectrometry of Biotinylated Self-Assembled Monolayers

et al. 1997

View full text Add to dashboard Cite

show abstract

“…However, LD produces significant molecular fragmentation and has poor reproducibility. Fragmentation can be reduced by using IR laser desorption followed by multiphoton ionization (MUPI) [18][19][20][21], but the technique requires sophisticated instrumentation and is not applicable to a broad range of compounds.…”

Section: Introductionmentioning

confidence: 99%

Coupling protocol for thin layer chromatography/matrix-assisted laser desorption ionization

1997

View full text Add to dashboard Cite

SummaryThis paper reports the investigation and optimization of the thin layer chromatography/matrix-assisted laser desorption/ionization (TLC-MALDI) coupling protocol. The fundamental coupling parameters which influence sensitivity and lateral analyte spreading are extraction solvent selection, extraction time, and pressure. Selection of the solvent is dependent upon its extraction efficiency, which has been correlated with extraction solvent Rf value. Maximum extraction efficiency occurs after two minutes for the analyte/solvent system studied. High extraction efficiency solvents cause significant lateral spreading of analyte; up to a three-fold increase in initial analyte spot size. Analyte recovery was found to be limited by the silica gel inter-particle porosity and the solvent extraction efficiency. For maintaining chromatographic resolution and maximizing signal intensity, extraction solvents with Rf values between 0.4 to 0.6 are optimal. The upper analyte recovery limit, using extraction solvents within this Rf range, is estimated at 22 %.

show abstract

“…However, conventional LD‐TLC/MS based on a UV laser suffers from extensive fragmentation due to the high laser power, which is required for the ionization of the analytes. Consequently, infrared (IR) laser desorption from TLC plates, followed by a post‐ionization step, was developed and applied by several groups 7–10…”

mentioning

confidence: 99%

Thin‐layer chromatography combined with diode laser desorption/atmospheric pressure chemical ionization mass spectrometry

Peng

Ahlmann

Kunze

et al. 2004

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

The desorption of an analyte by a continuous wave diode laser from a porous surface of a thin-layer plate covered with a graphite suspension is presented. The thermally desorbed analyte molecules are ionized in the gas phase by a corona discharge at atmospheric pressure. Therefore, both essential processes--the desorption and the ionization of analyte molecules, which are often performed in one step--are separated. The target preparation is easy and fast since no additional extraction process is required. The mass spectrometric background signal was mostly limited to the low mass range showing no interference with typical compounds of interest. In this study, the calmative and antihypertensive drug reserpine was chosen as model analyte, which is often used for specification of mass spectrometers. No fragmentation was observed because of efficient collisional cooling under atmospheric pressure. The influence of diode laser power and the composition of the graphite suspension were investigated, and a primary optimization was performed.

show abstract

Thin‐layer chromatography/laser desorption of peptides followed by multiphoton ionization time‐of‐flight mass spectrometry

Cited by 12 publications

References 20 publications

Surface Mass Spectrometry of Biotinylated Self-Assembled Monolayers

Surface Mass Spectrometry of Biotinylated Self-Assembled Monolayers

Coupling protocol for thin layer chromatography/matrix-assisted laser desorption ionization

Thin‐layer chromatography combined with diode laser desorption/atmospheric pressure chemical ionization mass spectrometry

Contact Info

Product

Resources

About