Thin-layer chromatography of steroidal alkaloids

Hunter, Irving R.; Walden, Mayo K.; Wagner, Joseph R.; Heftmanń, Erich

doi:10.1016/s0021-9673(00)81218-2

Cited by 29 publications

(8 citation statements)

References 3 publications

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“…The merits of different detection reagents have been presented [70]. For example, phosphomolybdic acid was less suitable for the determination of α-solanine in leaves, whereas the ceric sulphate-sulphuric acid reagent was less suitable for the detection of α-chaconine Solvent systems for the separation on silica gel have been developed [71] and the chromatographic variables reported in the literature have been reviewed and listed [72]. A recent review covers the analytical aspects, including TLC, of the major glycoalkaloids from potatoes and tomatoes [73].…”

Section: Methods Of Isolation and Analysismentioning

confidence: 99%

Steroidal Glycoalkaloids: Isolation, Structure, Analysis, and Biosynthesis

Ghisalberti

2006

Natural Product Communications

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In recent years, the steroidal glycoalkaloids have emerged as an important class of bioactive compounds. Their occurrence in a number of foods and their diverse biological activities has encouraged the development of more efficient procedures for their isolation, purification and analysis, and more sensitive methods of detection and structure determination. This review is mainly concerned with new approaches for the characterization of these metabolites. The formation of artifacts of isolation and purification is discussed. The limited information on their biosynthesis is summarized.

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Section: Methods Of Isolation and Analysismentioning

confidence: 99%

Steroidal Glycoalkaloids: Isolation, Structure, Analysis, and Biosynthesis

Ghisalberti

2006

Natural Product Communications

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“…Aglycones were extracted with either chloroform or benzene, and sugars were dissolved in distilled water. The aglycones were identified by their TLC mobility relative to tomatidine (ICN Pharmaceuticals) in three solvent systems (hexane: ethyl acetate [ 1: 1], CH 2 Cl 2 : methanol [9: 1], and Ch 2 Cl 2 : acetone [4: 1]) and by their characteristic color reactions when sprayed with 50% aqueous sulfuric acid and heated to 80° (Hunter et a!. 1976).…”

Section: Phytochemical Methodsmentioning

confidence: 99%

Seasonal Variation in Plant‐Insect Associations: Utilization of Solanum Dulcamara by Leptinotarsa Decemlineata

Hare

1983

Ecology

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In laboratory studies, Solanum dulcamara (nightshade) is one of the better host species for survival and growth of the Colorado potato beetle, Leptinotarsa decemlineata. The dynamics of L. decemlineata on S. dulcamara in the field, however, are far different than would be predicted from laboratory results. Therefore, a number of experiments were performed to determine the suitability of S. dulcamara for L. decemlineata under seasonal, field conditions. Field experiments showed that between 40 and 67% of first—generation adult females entered diapause without reproducing when fed S. dulcumara as both larvae and adults, although no adults entered diapause when fed S. tuberosum in both life—stages. Dispersal by first—generation adults from S. dulcamara was not observed in mark—recapture experiment. Survival of L. decemlineata on S. dulcamara did not differ significantly from survival on S. tuberosum during the part of the season corresponding to the development period of first—generation larvae. Survival on S. dulcamara was two—thirds lower during part of the season corresponding to the development period of the second—generation larvae, however. Emergence mass and larval development time varied less over the season on S. dulcamara than did survival. Analyses of extractable leaf protein, free amino acids, and glycoalkaloids showed significant seasonal variation in the concentrations of these S. dulcamara phytochemicals which are known to affect L. decemlineata feeding growth and survival. Multiple regression analyses between phytochemical concentration and larval survival showed that survival was significantly] positively associated with leaf nutritional quality (extractable protein and one free amino acid) during the first generation, and significantly negatively associated with leaf glycoalkaloid content during the second generation. Thus the seasonal variation in the suitability of S. dulcamara for L. decemlineata survival was most strongly associated with the two seasonally most variable S. dulcamara phytochemical factors. The unique and independent contribution of variation in either one to the variation in L. decemlineata survival, however, could not accurately be predicted without considering the simultaneous, independent variation in the other.

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“…Aqueous extract not hydrolyzed. d Solasodine aglycon was identified [TLC (Hunter et al, 1976), GLC, GLC-MS, MP, and mixed MP]. Composition of sugar moieties was determined by GLC (Varma et al, 1973).…”

Section: Methodsmentioning

confidence: 99%

Glycoalkaloids of wild, tuber-bearing Solanum species

Gregory¹,

Sinden²,

Osman³

et al. 1981

J. Agric. Food Chem.

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The total glycoalkaloid (TGA) levels and individual glycoalkaloid compositions of current potato cultivare do not represent a toxicological or teratogenic hazard. However, the widespread exploitation of wild, tuber-bearing Solanum species in potato breeding programs suggests the need for caution in the future. Some species contain much higher TGA levels than are found in Solanum tuberosum (Schreiber, 1963; Osman et al., 1978). There is also widespread occurrence among these wild species of glycoalkaloids which are not commonly associated with commercial varieties [for a review, see Schreiber (1968)]. Since TGA levels and individual glycoalkaloid compositions are largely under genetic control (Sanford and Sinden, 1972;McCollum and Sinden, 1979), and glycoalkaloids have been associated with undesirable potato flavor (Sinden and Deahl, 1976), mammalian toxicity and teratogenicity [for a review, see Kuc (1975)], careful consideration of glycoalkaloid contents in the parental species is highly desirable.The need for this was illustrated in the late 1960s when a Gr. Tuberosum cultivar Lenape, which had S. chacoense in its ancestry (Akeley et al., 1968

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Thin-layer chromatography of steroidal alkaloids

Cited by 29 publications

References 3 publications

Steroidal Glycoalkaloids: Isolation, Structure, Analysis, and Biosynthesis

Steroidal Glycoalkaloids: Isolation, Structure, Analysis, and Biosynthesis

Seasonal Variation in Plant‐Insect Associations: Utilization of Solanum Dulcamara by Leptinotarsa Decemlineata

Glycoalkaloids of wild, tuber-bearing Solanum species

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