Thin-layer chromatography of aflatoxins

Reddy, Tirumuru V.; Viswanathan, L.; Venkitasubramanian, T. A.

doi:10.1016/0003-2697(70)90487-2

Cited by 104 publications

(32 citation statements)

References 5 publications

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“…The plates were observed under UV light (at 366 nm) for detection of various aflatoxins by comparison with a standard after 5-6 min air drying of the plates. Chemical confirmation of aflatoxins was carried out by spraying 25% H 2 SO 4 (Reddy et al 1970;Stack and Pohland 1975).…”

Section: Methods Of Analysismentioning

confidence: 99%

Factors influencing fungal and aflatoxin levels in Turkish hazelnuts (Corylus avellanaL.) during growth, harvest, drying and storage: A 3-year study

Özay

Seyhan

Pembeci

et al. 2008

Food Additives & Contaminants: Part A

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The levels aflatoxins in Turkish hazelnuts have been monitored over a 3-years period (2002)(2003)(2004). Periodical sampling was made in 72 different orchards at different locations representative of the hazelnut-growing areas and post-harvest applications. Various parameters (aflatoxins, water activity, moulds) were analysed and environmental conditions (temperature and relative humidity) recorded during growing and at different stages of harvest and post-harvest processing, involving three different harvesting methods (collection in nets, from the ground, etc.) and four drying techniques (traditional sun-drying, mechanical drying, etc.). Fungal and aflatoxin analyses (HPLC) showed no significant difference except between samples which had been in contact with the ground and those which had not (at 95% confidence level). Aflatoxins levels from the orchard recorded a maximum of 0.77 AE 0.08 ng g À1 from a total of 1624 samples. Regarding harvesting and post-harvest processes, the only application where aflatoxins were detected was in samples which had been in direct contact with the ground (max. 3.18 AE 0.03 ng g À1 ). Aflatoxin formation was low during storage (max. 0.34 AE 0.003 ng g À1 ). As a result of mycological studies, a total of 5546 Aspergillus flavus (89%) and A. parasiticus (11%) species were isolated and identified from samples. The results indicated that harvesting hazelnuts into a canvas by shaking the trees, manual harvesting of mature hazelnuts where possible, use of jute instead of nylon sacks and mechanical drying technique would minimize aflatoxin levels in hazelnuts. These recommendations have been implemented and about 4000 people in the hazelnut industry have been trained in these practices.

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Section: Methods Of Analysismentioning

confidence: 99%

Factors influencing fungal and aflatoxin levels in Turkish hazelnuts (Corylus avellanaL.) during growth, harvest, drying and storage: A 3-year study

Özay

Seyhan

Pembeci

et al. 2008

Food Additives & Contaminants: Part A

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“…Benzene-methanol-acetic acid (24: 2: 1, v/v/v) was used as a mobile phase (18). For aflatoxins, one more solvent system, toluene-isoamyl alcohol-methanol (90: 32 : 2, v/v/v), was also used (15 The presence of mycotoxins was confirmed by spraying the developed TLC plates with suitable chemical reagents, i.e. for Afl-B1, 50% aqueous sulphuric acid (20); ochratoxin A, 24% aluminum chloride solution in 95% ethanol (S); zearalenone, p-anisaldehyde solution (70 ml methanol + 10 ml glacial acetic acid + 5 ml conc sulphuric acid + 0.5 ml p-anisaldehyde) and for citrinin, ammonia vapour and 14% boron trifluoride in ethanol (18).…”

Section: Methodsmentioning

confidence: 99%

Mycoflora, mycotoxin producibility and mycotoxins in traditional herbal drugs from india.

Roy

Chourasia

1990

J. Gen. Appl. Microbiol.

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“…The chromatoplates were observed under long UV light at 360 nm. The spot of aflatoxin B 1 was scrapped and quantitative estimation was done by spectrophotometer [5].…”

Section: Methodsmentioning

confidence: 99%

Effect of Homoeopathic Drugs to Control Growth and Production of <i>A. flavus</i>

Singh¹,

Kumari²,

Prasad³

2015

ABB

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Five common homoeopathic drugs viz., Belladonna, Bryonia, Colchicum, Colocynth and Lathyrus sat were selected and tested against growth and aflatoxin production of Aspergillus flavus. The result indicates that all five drugs suppressed the growth of A. flavus. The lower concentration of all the tested drugs induced maximum growth of fungi and maximum production of aflatoxin. However, the growth as well as aflatoxin production potentiality was considerably decreased when the concentration of the drug was increased. Out of all the five drugs, Bryonia was comparatively less effective with respect to inhibition in aflatoxin production. But Belladonna was found to be most effective drug on growth and aflatoxin production.

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Thin-layer chromatography of aflatoxins

Cited by 104 publications

References 5 publications

Factors influencing fungal and aflatoxin levels in Turkish hazelnuts (Corylus avellanaL.) during growth, harvest, drying and storage: A 3-year study

Factors influencing fungal and aflatoxin levels in Turkish hazelnuts (Corylus avellanaL.) during growth, harvest, drying and storage: A 3-year study

Mycoflora, mycotoxin producibility and mycotoxins in traditional herbal drugs from india.

Effect of Homoeopathic Drugs to Control Growth and Production of <i>A. flavus</i>

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Thin-layer chromatography of aflatoxins

Cited by 104 publications

References 5 publications

Factors influencing fungal and aflatoxin levels in Turkish hazelnuts (Corylus avellanaL.) during growth, harvest, drying and storage: A 3-year study

Factors influencing fungal and aflatoxin levels in Turkish hazelnuts (Corylus avellanaL.) during growth, harvest, drying and storage: A 3-year study

Mycoflora, mycotoxin producibility and mycotoxins in traditional herbal drugs from india.

Effect of Homoeopathic Drugs to Control Growth and Production of &lt;i&gt;A. flavus&lt;/i&gt;

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Effect of Homoeopathic Drugs to Control Growth and Production of <i>A. flavus</i>