Thin-Layer Chromatography-Image Analysis Method for the Simultaneous Quantification of Andrographolide and Related Diterpenoids inAndrographis paniculata

Phattanawasin, Panadda; Sotanaphun, Uthai; Burana-osot, Jankana

doi:10.1556/jpc.27.2014.2.12

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…thin-layer chromatography [8], micellar electrokinetic capillary chromatography [9], infrared spectroscopy [7], and nuclear magnetic resonance spectroscopy [10] to develop methods for quantifying the levels of AP and DAP in A. paniculata. Notably, HPLC is recognized as the standard method for determining component content of A. paniculata according to the Chinese Pharmacopoeia [1], and is the most widely used method in A. paniculata industry.…”

Section: Introductionmentioning

confidence: 99%

Rapid Determination of Andrographolide and Dehydroandrographolide in Andrographis paniculata by HPLC-UV at Equal Absorption Wavelength

Huang,

Lei,

et al. 2024

J. Exp. Clin. Appl. Chin. Med

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A rapid and reference component saving method for the determination of andrographolide and dehydroandrographolide in Andrographis paniculata was developed in this study. The sample was prepared using matrix solid-phase dispersion and analyzed by an Agilent Poroshell 120 EC-C18 column (50 mm × 4.6 mm, 2.7 μm) with a water-acetonitrile mobile phase. The equal absorption wavelength of two components was confirmed to be 240 nm using a combination of ultraviolet spectrophotometer and high performance liquid chromatograph. Results showed that andrographolide and dehydroandrographolide exhibited good linearity in their concentration ranges (r ≥ 0.9999), with the limit of quantification of 80.85 μg/g and 80.63 μg/g, respectively. Two components also had a good precision as their relative standard deviation (RSD) value less than 1.00%. The average recoveries of two components were 102.80% (RSD = 1.54%) and 100.70% (RSD = 1.91%), respectively. The developed method shared the same determination result with external standard method and Chinese Pharmacopoeia, which took a total of 10 minutes, consumed 8.5 mL harmful solvent, and only used one reference component to determine the content of two components. This method is characterized by rapid determination and low reference component consumption in the rapid study on the main active components of Andrographis paniculata and its products, which contributes to the quality evaluation of Andrographis paniculata.

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Section: Introductionmentioning

confidence: 99%

Rapid Determination of Andrographolide and Dehydroandrographolide in Andrographis paniculata by HPLC-UV at Equal Absorption Wavelength

Huang,

Lei,

et al. 2024

J. Exp. Clin. Appl. Chin. Med

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“…TLC-image analysis method using a common computer technology for simultaneous quantification of andrographolide and its related diterpenoids in A. paniculata has been developed in our previous study [14]. Since A. paniculata plant and formulations have been widely used as a common herbal drug for the treatment of colds, flu and uncomplicated respiratory infections, an investigation on the stability of its major active constituent, andrographolide, would be of interest.…”

Section: Introductionmentioning

confidence: 99%

Stability-indicating TLC—image analysis method for determination of andrographolide in bulk drug and Andrographis paniculata formulations

Phattanawasin

Burana-osot

Sotanaphun

et al. 2016

Acta Chromatographica

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Summary.A simple and rapid thin-layer chromatographic (TLC)-image analysis method was developed for stability-indicating studies and determination of andrographolide in bulk drug and in Andrographis paniculata formulations. Good chromatographic separation of andrographolide and the degradation products under various stress conditions was achieved on a silica gel G60 F254 TLC plate with the use of two mobile phases, dichloromethane-toluene-ethanol (6:3:1, v/v/v) and toluene-ethyl acetate-formic acid (5:3.5:1.5, v/v/v) and p-anisaldehyde as visualization reagent. Image analysis of the scanned TLC plate was performed by Sorbfil TLC Videodensitometer, UN-SCAN-IT, JustTLC, and ImageJ software to measure the quantity of the dark bluecolored band of andrographolide on a TLC plate. The TLC-image analysis method was validated in terms of specificity, linearity, sensitivity, precision, accuracy, and robustness and was also applied for determination of the amount of andrographolide in A. paniculata formulations and the content of andrographolide remained in the samples under forced degradations. The analytical results determined by the TLC-image analysis method, TLC-densitometry, and high-performance liquid chromatography (HPLC) methods were compared and found to be not significantly different.

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Thin-Layer Chromatography-Image Analysis Method for the Simultaneous Quantification of Andrographolide and Related Diterpenoids inAndrographis paniculata

Cited by 2 publications

References 13 publications

Rapid Determination of Andrographolide and Dehydroandrographolide in <i>Andrographis paniculata</i> by HPLC-UV at Equal Absorption Wavelength

Rapid Determination of Andrographolide and Dehydroandrographolide in <i>Andrographis paniculata</i> by HPLC-UV at Equal Absorption Wavelength

Stability-indicating TLC—image analysis method for determination of andrographolide in bulk drug and Andrographis paniculata formulations

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