Abstract:Summary.A simple and rapid thin-layer chromatographic (TLC)-image analysis method was developed for stability-indicating studies and determination of andrographolide in bulk drug and in Andrographis paniculata formulations. Good chromatographic separation of andrographolide and the degradation products under various stress conditions was achieved on a silica gel G60 F254 TLC plate with the use of two mobile phases, dichloromethane-toluene-ethanol (6:3:1, v/v/v) and toluene-ethyl acetate-formic acid (5:3.5:1.5,… Show more
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