Thin-layer chromatography characteristics of β-carotene

Checheta, O. V.; Safonova, E. F.; Сливкин, А. И.

doi:10.1007/s11094-012-0790-4

Cited by 5 publications

(6 citation statements)

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“…The productivity of this strain was 44.4 ± 1.2 mg/kg of β-carotene. The synthesized pigment was identified by a TLC analysis using lycopene and β-carotene as reference samples as well as by a spectrophotometric analysis with the wavelengths corresponding to lycopene (474 nm) and β-carotene (450 nm) [22,25]. The TLC chromatogram showed that the biomass contained only one pigment, whose Rf coincided with that of the β-carotene reference sample (Table 2).…”

Section: Breeding Of M Neoaurum Ac-501/22 Characterized By An Enhance...mentioning

confidence: 99%

Enhanced β-Carotene Production in Mycolicibacterium neoaurum Ac-501/22 by Combining Mutagenesis, Strain Selection, and Subsequent Fermentation Optimization

Yaderets,

Karpova,

Glagoleva

et al. 2023

Fermentation

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A continuing interest of scientists regarding the development of new β-carotene production technologies is due to the high biological activity of this compound and its wide application range. Bacteria are considered among the possible β-carotene producers convenient for industrial use. The purpose of this study was to develop a Mycolicibacterium neoaurum strain with an enhanced ability for β-carotene production and to optimize the fermentation conditions to improve the final yield of the target compound. Using chemical mutagenesis with N-nitroso-N-methylurea along with further strain selection, a M. neoaurum strain Ac-501/22, whose productivity was 2.7-fold higher than that of the parental strain Ac-501, was developed. The effect of nitrogen and carbon sources as well as cultivation conditions on the growth of M. neoaurum Ac-501/22 and β-carotene production were studied to select the optimal fermentation regime. Due to an increase in the temperature of cultivation from 30 to 35 °C, replacement of glucose with glycerin (20.0 g/L) and degreased soybean flour with powdered milk (10.0 g/L), and increase in the urea content from 0.5 to 1.0 g/L, the level of β-carotene production was improved to 183.0 mg/kg that was 35% higher than in the control. Further strain fermentation in a 3 L bioreactor using an optimized medium with the pH level maintained at 7.0–7.2 and 50% pO2 provided the maximum output of the target compound (262.4 mg/kg of dry biomass) that confirmed the prospects of the developed strain as an industrial β-carotene producer.

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Section: Breeding Of M Neoaurum Ac-501/22 Characterized By An Enhance...mentioning

confidence: 99%

Enhanced β-Carotene Production in Mycolicibacterium neoaurum Ac-501/22 by Combining Mutagenesis, Strain Selection, and Subsequent Fermentation Optimization

Yaderets,

Karpova,

Glagoleva

et al. 2023

Fermentation

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“…The absorption dependence of the band at 290 nm wavelengths on TP concentration was linear and obeyed the Beer-Lambert law [59]; thus, there were not any intermolecular interactions between the components of solution. However, the hydroxyl group of TP can partake in the formation of intermolecular hydrogen bonds [60]. The formation of intermolecular H-bonds was evidenced by the wide absorption band of stretching vibrations of the OH-group at 3700-3100 cm −1 in its IR spectrum (Figure 4).…”

Section: Encapsulation Tp In Vp-copolymer and Characterization Of Tp-npsmentioning

confidence: 99%

“…In the range of 1610-1580 cm −1 , stretching vibrations of the C=C bond of the aromatic nucleus are seen. The characteristic absorption bands at 1097 and 813 cm −1 belong to the tetrahydropyran cycle and the band at 1169 cm −1 concerns the tocol fragment [60]. According to [61], the TP biological activity is associated with these structural fragments.…”

Section: Encapsulation Tp In Vp-copolymer and Characterization Of Tp-npsmentioning

confidence: 99%

Design and Investigation of New Water-Soluble Forms of α-Tocopherol with Antioxidant and Antiglycation Activity Using Amphiphilic Copolymers of N-Vinylpyrrolidone

et al. 2023

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Water-soluble forms of α-tocopherol (TP) as an effective antioxidant were obtained by encapsulating it into nanoparticles (NPs) of amphiphilic copolymers of N-vinylpyrrolidone with triethylene glycol dimethacrylate (CPL1-TP) and N-vinylpyrrolidone with hexyl methacrylate and triethylene glycol dimethacrylate (CPL2-TP) synthesized by radical copolymerization in toluene. The hydrodynamic radii of NPs loaded with TP (3.7 wt% per copolymers) were typically ca. 50 or 80 nm depending on copolymer composition, media, and temperature. Characterization of NPs was accomplished by transmission electron microscopy (TEM), IR-, and 1H NMR spectroscopy. Quantum chemical modeling showed that TP molecules are capable to form hydrogen bonds with donor groups of the copolymer units. High antioxidant activity of both obtained forms of TP has been found by the thiobarbituric acid reactive species and chemiluminescence assays. CPL1-TP and CPL2-TP effectively inhibited the process of spontaneous lipid peroxidation as well as α-tocopherol itself. The IC50 values of luminol chemiluminescence inhibition were determined. Antiglycation activity against vesperlysine and pentosidine-like AGEs of TP water-soluble forms was shown. The developed NPs of TP are promising as materials with antioxidant and antiglycation activity and can be used in various biomedical applications.

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“…Также для идентификации флавоноидов и фенольных соединений использовали метод тонкослойной хроматографии [18,19]. Для ее проведения готовили стандартные растворы (СО) рутина и хлорогеновой кислоты 0,01% в спирте этиловом 96%.…”

Section: материалы и методыunclassified

Technology of Obtaining Liquid Extract of Wormwood and Determination of its Quality Indicators

Сушинская,

Голяк

2023

Рецепт

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Цель исследования. Получение жидкого экстракта травы полыни горькой 1:1 и определение его показателей качества. Материалы и методы. Объектом исследования выбрали траву полыни горькой производства ООО «Калина» (Республика Беларусь). Для получения 5 серий жидкого экстракта использовали метод реперколяции. Для полученных экстрактов определяли относительную плотность и сухой остаток по общепринятым фармакопейным методикам, для подтверждения наличия флавоноидов, сесквитерпеновых лактонов и фенольных соединений в экстракте проводили качественные реакции, а также использовали метод тонкослойной хроматографии. Количественное определение суммы флавоноидов в пересчете на рутин определяли спектрофотометрическим методом по валидированной методике. Результаты. Для получения жидкого экстракта полыни горькой травы 1:1 предложены следующие условия: экстрагент – 70%-ный этиловый спирт; метод экстрагирования – реперколяция с делением сырья на равные части в батарее из 3 перколяторов; температура экстракции – комнатная; размеры измельченного сырья – 1–3 мм; коэффициент спиртопоглощения – 4,4. Сухой остаток – 4,72±0,36%, относительная плотность составила 0,9103±0,0057, содержание суммы флавоноидов в жидком экстракте полыни горькой в пересчете на рутин составило 0,154±0,004%. Заключение. Предложена технология получения жидкого экстракта полыни горькой 1:1. Изучены основные фармакопейные показатели качества экстракта, такие как подлинность и описание, количественное определение, сухой остаток и относительная плотность. Жидкий экстракт является перспективным экстракционным препаратом для дальнейшей разработки топических лекарственных форм противовоспалительного и обезболивающего действия. The purpose of the study. To obtain a liquid extract of the herb wormwood 1:1 and determine its quality indicators. Materials and methods. The object of the study was chosen wormwood herb produced by LLC "Kalina" (Republic of Belarus). To obtain 5 series of liquid extract, the method of repercolation was used. For the obtained extracts, the relative density and dry residue were determined according to generally accepted pharmacopoeial methods, qualitative reactions were carried out to confirm the presence of flavonoids, sesquiterpene lactones and phenolic compounds in the extract, and thin-layer chromatography was also used. Quantitative determination of the amount of flavonoids in terms of rutin was determined by the spectrophotometric method according to a validated technique. Results. To obtain a liquid extract of wormwood bitter herb 1:1, the following conditions are proposed: extractant – 70% ethyl alcohol; extraction method – repercolation with the division of raw materials into equal parts in a battery of 3 percolators; extraction temperature – room; size of crushed raw materials – 1–3 mm; alcohol absorption coefficient – 4.4. Dry residue – 4.72±0.36%, relative density was 0.9103±0.0057, the content of the sum of flavonoids in liquid the extract of wormwood in terms of rutin was 0.154±0.004%. Conclusion. The technology of obtaining a liquid extract of wormwood 1:1 is proposed. The main pharmacopoeial indicators of the quality of the extract such as authenticity and description, quantitative determination, dry residue and relative density are studied. Liquid extract is a promising extraction preparation for the further development of topical dosage forms of anti-inflammatory and analgesic action.

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Thin-layer chromatography characteristics of β-carotene

Cited by 5 publications

References 1 publication

Enhanced β-Carotene Production in Mycolicibacterium neoaurum Ac-501/22 by Combining Mutagenesis, Strain Selection, and Subsequent Fermentation Optimization

Enhanced β-Carotene Production in Mycolicibacterium neoaurum Ac-501/22 by Combining Mutagenesis, Strain Selection, and Subsequent Fermentation Optimization

Design and Investigation of New Water-Soluble Forms of α-Tocopherol with Antioxidant and Antiglycation Activity Using Amphiphilic Copolymers of N-Vinylpyrrolidone

Technology of Obtaining Liquid Extract of Wormwood and Determination of its Quality Indicators

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