Thin-layer chromatographic procedures for lipid separation

Touchstone, Joseph C.

doi:10.1016/0378-4347(95)00232-8

Cited by 147 publications

(59 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3 Alternatively, morphological detail has been obtained by the labeling of selected molecules by fluorescent or electron-dense labels for subsequent light or electron microscopy. Since most current models of cell membranes indicate substantial heterogeneity in composition among and within the different membranes of a cell, the preparation of a homogenate presumably precludes the study of important aspects of membrane composition.…”

Section: Conventional Methods For the Study Of Membrane Lipids New Dmentioning

confidence: 99%

ToF‐SIMS imaging of lipids in cell membranes

Johansson¹

2006

Surface & Interface Analysis

View full text Add to dashboard Cite

show abstract

Section: Conventional Methods For the Study Of Membrane Lipids New Dmentioning

confidence: 99%

ToF‐SIMS imaging of lipids in cell membranes

Johansson¹

2006

Surface & Interface Analysis

View full text Add to dashboard Cite

show abstract

“…The radioactivity of each fraction was also measured in a ␤ counter. The analysis of nonhydrolyzed lipids was performed as described (22). The initial chloroform extract was loaded on an aminopropyl column (Supelco).…”

Section: Methodsmentioning

confidence: 99%

Platelet-derived Growth Factor Stimulates Membrane Lipid Synthesis Through Activation of Phosphatidylinositol 3-Kinase and Sterol Regulatory Element-binding Proteins

Demoulin

Ericsson

Kallin

et al. 2004

Journal of Biological Chemistry

111

105

View full text Add to dashboard Cite

We analyzed the transcriptional program elicited by stimulation of normal human fibroblasts with plateletderived growth factor (PDGF) using cDNA microarrays. 103 significantly regulated transcripts that had not been previously linked to PDGF signaling were identified. Among them, a cluster of genes involved in fatty acid and cholesterol biosynthesis, including stearoyl-CoA desaturase (SCD), fatty acid synthase, and hydroxymethylglutaryl-CoA synthase (HMGCS), was up-regulated by PDGF after 24 h of treatment, and their expression correlated with increased membrane lipid production. These genes are known to be controlled by sterol regulatory element-binding proteins (SREBP). PDGF increased the amount of mature SREBP-1 and regulated the promoters of SCD and HMGCS in an SREBP-dependent manner. In line with these results, blocking SREBP processing by addition of 25-hydroxycholesterol blunted the effects of PDGF on lipogenic enzymes. SREBP activation was dependent on the phosphatidylinositol 3-kinase (PI3K) pathway, as judged from the effects of the inhibitor LY294002 and mutation of the PDGF␤ receptor tyrosines that bind the PI3K adaptor subunit p85. Fibroblast growth factors (FGF-2 and FGF-4) and other growth factors mimicked the effects of PDGF on NIH3T3 and human fibroblasts. In conclusion, our results suggest that growth factors induce membrane lipid synthesis via the activation SREBP and PI3K.

show abstract

“…Phospholipids were resolved by TLC on silica gel plates using CHCl 3 /CH 3 OH/28% NH 4 OH (65:25:4) as a developing system (28). Lipid spots were visualized by placing the plate in a tank saturated with iodine vapor.…”

Section: Incorporation Of Radiolabeled Acetate Into Lipid Fractionsmentioning

confidence: 99%

Oleic acid is a potent inhibitor of fatty acid and cholesterol synthesis in C6 glioma cells

Natali

Siculella

Salvati

et al. 2007

Journal of Lipid Research

View full text Add to dashboard Cite

Glial cells play a pivotal role in brain fatty acid metabolism and membrane biogenesis. However, the potential regulation of lipogenesis and cholesterologenesis by fatty acids in glial cells has been barely investigated. Here, we show that physiologically relevant concentrations of various saturated, monounsaturated, and polyunsaturated fatty acids significantly reduce [1-14 C]acetate incorporation into fatty acids and cholesterol in C6 cells. Oleic acid was the most effective at depressing lipogenesis and cholesterologenesis; a decreased label incorporation into cellular palmitic, stearic, and oleic acids was detected, suggesting that an enzymatic step(s) of de novo fatty acid biosynthesis was affected. To clarify this issue, the activities of acetylcoenzyme A carboxylase (ACC) and FAS were determined with an in situ digitonin-permeabilized cell assay after incubation of C6 cells with fatty acids. ACC activity was strongly reduced (?80%) by oleic acid, whereas no significant change in FAS activity was observed. Oleic acid also reduced the activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR). The inhibition of ACC and HMGCR activities is corroborated by the decreases in ACC and HMGCR mRNA abundance and protein levels. The downregulation of ACC and HMGCR activities and expression by oleic acid could contribute to the reduced lipogenesis and cholesterologenesis.-Natali, F., L. Siculella, S. Salvati, and G. V. Gnoni. Oleic acid is a potent inhibitor of fatty acid and cholesterol synthesis in C6 glioma cells. J. Lipid Res. 2007Res. . 48: 1966Res. -1975. After white adipose tissue, the brain is the organ with the highest lipid content of the body. The biosynthesis and deposition of lipids play an important role in maintaining brain structure and function, for example, during development-associated biogenesis of neural cell membranes. It is well established that alterations in lipid metabolism are the cause of or are associated with many neurological diseases (1-3).Astrocytes, the major class of glial cells in the mammalian brain, play an active role in brain metabolism. These cells surround intraparenchymal blood capillaries so that they represent the first cellular barrier for nutrients and other substances entering the brain system. A metabolic coupling between astrocytes and neurons to maintain energy metabolism homeostasis has been described (4, 5). Metabolic regulation in the brain has been investigated extensively, and those studies focused mostly on carbohydrate and amino acid metabolism (for review, see Ref. 6). During neuronal activity, glucose taken up by astrocytes is converted into lactate, which is then released into the extracellular space to be used by neurons (6). Regarding lipid metabolism, astroglial ketone body synthesis, showing characteristics strikingly similar to those of hepatic ketogenesis (7), may represent an important pathway for brain energy production and/or biosynthetic processes. The involvement of fatty acids in cell death pathways, particularly in the con...

show abstract

Thin-layer chromatographic procedures for lipid separation

Cited by 147 publications

References 97 publications

ToF‐SIMS imaging of lipids in cell membranes

ToF‐SIMS imaging of lipids in cell membranes

Platelet-derived Growth Factor Stimulates Membrane Lipid Synthesis Through Activation of Phosphatidylinositol 3-Kinase and Sterol Regulatory Element-binding Proteins

Oleic acid is a potent inhibitor of fatty acid and cholesterol synthesis in C6 glioma cells

Contact Info

Product

Resources

About