ToF‐SIMS imaging of lipids in cell membranes

Johansson, Björn

doi:10.1002/sia.2361

Cited by 62 publications

(46 citation statements)

References 81 publications

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“…Other than primary metabolites, lipid distributions are of interest to researchers because they have been reported to regulate a variety of biological functions in the CNS, including energy storage, membrane composition, and chemical signaling. 25,30,31 In Figure 2f, the ion at m/z 369.3519 was identified as cholesterol with a neutral loss of water, agreeing with the previous observation of an m/z 369.3 peak by Jackson et al using a MRMS MALDI-TOF. 32 The abundance of the cholesterol peak was higher in brain stem regions such as the medulla, pons, and midbrain, as shown in Figure 2f.…”

Section: ■ Results and Discussionsupporting

confidence: 89%

Visualizing Neurotransmitters and Metabolites in the Central Nervous System by High Resolution and High Accuracy Mass Spectrometric Imaging

Wang²,

Greer³

et al. 2013

ACS Chem. Neurosci.

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ABSTRACT:The spatial localization and molecular distribution of metabolites and neurotransmitters within biological organisms is of tremendous interest to neuroscientists. In comparison to conventional imaging techniques such as immunohistochemistry, matrixassisted laser desorption/ionization (MALDI) mass spectrometric imaging (MSI) has demonstrated its unique advantage by directly localizing the distribution of a wide range of biomolecules simultaneously from a tissue specimen. Although MALDI-MSI of metabolites and neurotransmitters is hindered by numerous matrixderived peaks, high-resolution and high-accuracy mass spectrometers (HRMS) allow differentiation of endogenous analytes from matrix peaks, unambiguously obtaining biomolecular distributions. In this study, we present MSI of metabolites and neurotransmitters in rodent and crustacean central nervous systems acquired on HRMS. Results were compared with those obtained from a medium-resolution mass spectrometer (MRMS), tandem time-offlight instrument, to demonstrate the power and unique advantages of HRMSI and reveal how this new tool would benefit molecular imaging applications in neuroscience.

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Section: ■ Results and Discussionsupporting

confidence: 89%

Visualizing Neurotransmitters and Metabolites in the Central Nervous System by High Resolution and High Accuracy Mass Spectrometric Imaging

Wang²,

Greer³

et al. 2013

ACS Chem. Neurosci.

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“…Time-of-Xight secondary ion mass spectrometry (ToF-SIMS) is a surface analytical method, which due to recent instrumentation developments has emerged as a promising method for chemical microanalysis of biological samples (Belu et al 2003;Winograd 2005;Johansson 2006). BrieXy, mass spectra are recorded from the outermost molecular layers of a solid sample, such as a biological cell or tissue sample, by scanning a pulsed, focused, highenergy (primary) ion beam across the sample surface.…”

Section: Introductionmentioning

confidence: 99%

Chemical analysis of osmium tetroxide staining in adipose tissue using imaging ToF-SIMS

Belazi

Solé-Domènech

Johansson

et al. 2009

Histochem Cell Biol

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Osmium tetroxide (OsO4) is a commonly used stain for unsaturated lipids in electron and optical microscopy of cells and tissues. In this work, the localization of osmium oxide and specific lipids was independently monitored in mouse adipose tissue by using time-of-flight secondary ion mass spectrometry with Bi cluster primary ions. Substance-specific ion images recorded after OsO4 staining showed that unsaturated C18 fatty acids were colocalized with osmium oxide, corroborating the view that osmium tetroxide binds to unsaturated lipids. In contrast, saturated fatty acids (C14, C16 and C18) and also unsaturated C16 fatty acids show largely complementary localizations to osmium oxide. Furthermore, the distributions of saturated and unsaturated diglycerides are consistent with the specific binding of osmium oxide to unsaturated C18 fatty acids. The abundance of ions, characteristic of phospholipids and proteins, is strongly decreased as a result of the osmium staining, suggesting that a large fraction of these compounds are removed from the tissue during this step, while ions related to fatty acids, di- and triglycerides remain strong after osmium staining. Ethanol dehydration after osmium staining results in more homogeneous distributions of osmium oxide and unsaturated lipids. This work provides detailed insight into the specific binding of osmium oxide to different lipids.

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“…Also, SIMS can be used for depth-profiling, in effect creating a 3D image [46] Some molecules however can be observed intact with SIMS [52-54]. It has been possible to study intact membrane lipids and proteins by ToF-SIMS at the single cell level [55,56] and the analysis of unlabeled molecular components in lipid monolayers [57,58]. It is for these reasons that SIMS IMS is being explored as a tool in microbiology as it is the highest spatial resolution IMS that currently exists [59-64].…”

Section: Microbial Imaging Mass Spectrometrymentioning

confidence: 99%

Emerging mass spectrometry techniques for the direct analysis of microbial colonies

Fang

Dorrestein

2014

Current Opinion in Microbiology

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One of the emerging areas in microbiology is detecting specialized metabolites produced by microbial colonies and communities with mass spectrometry. In this review/perspective, we illustrate the emerging mass spectrometry methodologies that enable the interrogation of specialized metabolites directly from microbial colonies. Mass spectrometry techniques such as imaging mass spectrometry and real-time mass spectrometry allow two and three dimensional visualization of the distribution of metabolites, often with minimal sample pretreatment. The speed in which molecules are captured using these methods requires the development of new molecular visualization tools such as molecular networking. Together, these tools are beginning to provide unprecedented insight into the chemical world that microbes experience.

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ToF‐SIMS imaging of lipids in cell membranes

Abstract: Secondary ion mass spectrometry (SIMS) has been in use in

Cited by 62 publications

References 81 publications

Visualizing Neurotransmitters and Metabolites in the Central Nervous System by High Resolution and High Accuracy Mass Spectrometric Imaging

Visualizing Neurotransmitters and Metabolites in the Central Nervous System by High Resolution and High Accuracy Mass Spectrometric Imaging

Chemical analysis of osmium tetroxide staining in adipose tissue using imaging ToF-SIMS

Emerging mass spectrometry techniques for the direct analysis of microbial colonies

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