Thin-layer chromatographic identification of aminoglycoside antibiotics

Claes, P.; Vanderhaeghe, Hubert

doi:10.1016/s0021-9673(00)85062-1

Cited by 9 publications

(3 citation statements)

References 7 publications

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“…Ninhydrin (Claes, 1982), fast violet (Oka, 1984) etc. have been used as detection reagents for antibiotics; these are considered to have certain disadvantages, e.g.…”

Section: Resultsmentioning

confidence: 99%

TLC separation of certain tetracycline and amino glycopeptide antibiotics

Bhushan

Ali

1992

Biomedical Chromatography

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The separation of tetracycline and amino glycopeptide antibiotics was achieved on silica gel thin layers. Tetracycline antibiotics were resolved on a Co+2 (1.0%) impregnated silica gel layer using ethanol:acetic acid:water (10:6:6, v/v/v) as the mobile phase. Amino glycopeptide antibiotics were separated on an untreated silica gel layer using the mobile phase n-butanol:formic acid:water (6:5:7, v/v/v). The spots of these antibiotics were located by exposing the chromatoplate to iodine vapours.

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“…Ninhydrin (Claes, 1982), fast violet (Oka, 1984) etc. have been used as detection reagents for antibiotics; these are considered to have certain disadvantages, e.g.…”

Section: Resultsmentioning

confidence: 99%

TLC separation of certain tetracycline and amino glycopeptide antibiotics

Bhushan

Ali

1992

Biomedical Chromatography

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“…The KH 2 PO 4 solution at 7.5% was then chosen for plate development on the TLC-bioautographic system as recommended by Isnaeni (2005). The lower concentration of the solvent was originally reported by Claes & Vanderhaeghe (1982), by which the streptomycin could be separated from eight other aminoglycoside antibiotics using 15% and 10% aqueous solution of KH 2 PO 4 at pH 4.4 and 4.5 respectively. This solvent system gave Rf value of 0.66 and 0.56 respectively.…”

Section: Accuracy and Precisionmentioning

confidence: 98%

“…The TLCbioautography method of commonly used for bioactivity screening purposed in natural products (Choma & Edyta, 2011). The method validation for determination of streptomycin by TLC-bioautography has not been reported, although chromatogram profile of the aminoglycoside antibiotics using several solvent system has been reported by Claes & Vanderhaeghe (1982).…”

Section: Introductionmentioning

confidence: 99%

Validation of Thin-Layer Chromatography-Bioautographic Method for Determination of Streptomycin

Isnaeni

Astuti

Yuwono³

2018

JFIKI

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Background: A simple bio-assay for determination of streptomycin hyphenated with planar chromatography techniques was developed. Objective: This study aims to validate the method for identification and determination of streptomycin in injection preparations with TLC-bioautography. Methods: Thin Layer Chromatography (TLC) was performed on the silica Gel GF-254 using KH2PO4 solution as mobile solvent. The visualization was performed by spraying 2% resorcinol. Direct bi autography was developed using Escherichia coli ATCC 25922 as a bacterial test, grown on the nutrient agar medium at 37oC for 24 hours. The method was validated corresponding to linearity, limit of detection (LOD), intra day precision, and accuracy parameters. The accuracy was measured using streptomycin injection as a sample. Results: The Results showed that the KH2PO4 solution at 7.5% concentration was found to be the optimized solvent with Rf value of 0.5. The linear equation was y = 10.176x + 4.046 at 150 - 350 µg/mL concentration range with the linearity coefficient, Limit of Detection, accuracy, and variation coefficient were 0.9907; 40 ppm; 96.37 + 2.22% (with an RSD value of 2.31%); and 1.63 respectively. Conclusion: The prospective TLC-bioautographic method was applied for the identification and determination of streptomycin in a preparation using a single eluent KH2PO4. The eluent system optimization remains necessary for the identification and determination of the mixture of streptomycin with other antibiotics, such as aminoglycoside groups.

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