Thin-layer chromatographic determination of imipramine and desipramine in human plasma and urine at single—dose levels

Sistovaris, N.; Dagrosa, E. E.; Keller, A. J.

doi:10.1016/s0378-4347(00)84844-6

Cited by 9 publications

(2 citation statements)

References 12 publications

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“…Several analytical methods have been reported for the determination of IMH in biological fluids and/or pharmaceutical formulations. These include chromatographic techniques like HPLC [3][4][5][6][7][8][9], TLC [10], GC [11][12][13][14], LC with direct injection and electrochemical detection [15], adsorptive stripping voltammetry [16], chemometric methods [17], flow-injection extraction spectrophotometry [18], derivative spectrophotometry [19,20], and visible spectrophotometry [20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

“…To our present knowledge, no validated stability indicating UPLC assay method for the determination of IMH in pharmaceutical formulation was available in the literature. Though the numbers of liquid [3][4][5][6][7][8][9], thin layer [10], gas chromatographic methods [11][12][13][14], and LC with direct injection and electrochemical detection [15] earlier for IMH, no attempt has been made to apply this new type of ultra performance liquid chromatography for its determination in pharmaceuticals.…”

Section: Introductionmentioning

confidence: 99%

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Development and Validation of a Stability Indicating RP-UPLC Method for Analysis of Imipramine Hydrochloride in Pharmaceuticals

Deepakumari

Vinay

Revanasiddappa

2013

ISRN Analytical Chemistry

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The objective of the current study was the development of a simple, rapid, and accurate isocratic reverse-phase ultra-performance liquid chromatographic (RP-UPLC) method for the routine control analysis of imipramine hydrochloride (IMH) in bulk drug and in pharmaceutical formulations. This work was carried out in order to reduce analysis time and maintaining good efficiency which in turn is focused on high-speed chromatographic separations. The method was developed using Waters Acquity BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with mobile phase consisting of a mixture of acetonitrile and ammonium acetate buffer of pH-5 (80 : 20, v/v/v). UV detection was performed at 220 nm for eluted compound. An excellent linearity was observed in the concentration range 0.2–3 µg/mL IMH with a regression coefficient () value of 0.9999. The method developed was validated and forced degradation was performed as per ICH guidelines. The limit of detection () was 0.2532 ng/mL and the limit of quantitation () was found to be 0.7672 ng/mL. The drug IMH was subjected to hydrolytic, acidic, basic, thermal, photolytic, and oxidative stress conditions according to ICH regulations. IMH was found to be stable in basic, thermal, and photolytic conditions and degrades in acidic, hydrolytic, and oxidative stress conditions.

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