Thin-Layer Chromatographic Detection of Phosphorothionate and Phosphorothiolothionate Pesticides Using 4-Amino-N,N-Diethylaniline

Pasha, Akmal; Vijayashankar, Y. N.; Karanth, N. G.

doi:10.1093/jaoac/79.4.1009

Cited by 8 publications

(2 citation statements)

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“…Different chromatographic methods followed for the determination of Pirimiphos methyl in food and environmental samples [7][8]. For example, limits of detection (LOD) between 0.05 and 0.5 ng/ml were reached using thin layer chromatography (TLC) [9]; also high performance liquid chromatography (HPLC) with LOD of 16 ng/ml for the analysis of Pirimiphos methyl in water [10][11]. In addition, gas chromatography (GC) coupled to nitrogen/phosphorous (NPD) [12][13], atomic emission (AED) [14], electron capture (ECD) [15] or flame photometric detectors (FPD) [16] have also been applied for the determination of Pirimiphos methyl.…”

Section: Pirimiphos Methylmentioning

confidence: 99%

Validation of HPLC Method for Quantitative Determination of Pirimiphos Methyl

Tamilselvan¹,

Joseph²,

Mugunthan³

et al. 2014

ILCPA

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The objective of this research to optimise the HPLC method was developed for quantitative determination of Pirimiphos-methyl. Chromatographic separation was achieved on a 250 x 4.6 mm i.d. reversed phase column Qualisil BDS 5u C18, Using deionized acetonitrile:water in the ratio of 85:15 v/v respectively as mobile phase. The eluent was monitored at 254 nm. A sharp peak was obtained for the Pirimiphos-methyl at 9.29 min. The UV Spectrophotometric method performed at 254 nm after full scan analysis using methanol as a solvent. The result revealed that both methods are suitable to carry out routine analysis of Pirimiphos methyl, However HPLC results showed high precise, accurate and sensitive than the UV Spectrophotometer. Hence HPLC method is suitable for trace analysis of Pirimiphos methyl in environmental samples.

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Section: Pirimiphos Methylmentioning

confidence: 99%

Validation of HPLC Method for Quantitative Determination of Pirimiphos Methyl

Tamilselvan¹,

Joseph²,

Mugunthan³

et al. 2014

ILCPA

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“…Different chromatographic methods for the determination of chlorpyrifos in food and environmental samples [6][7]. For example, limits of detection (LOD) between 0.05 and 0.5 ng/ml were reached using thin layer chromatography (TLC) [8]; also high performance liquid chromatography (HPLC) with LOD of 16 ng/l for the analysis of chlorpyrifos in water [9]. In addition, gas chromatography (GC) coupled to nitrogen/phosphorous (NPD) [10][11], atomic emission (AED) [13], electron capture (ECD) [13] or flame photometric detectors (FPD) [14], have also been applied for the determination of chlorpyrifos.…”

Section: Introductionmentioning

confidence: 99%

Validation of UV Spectrophotometric and HPLC Methods for Quantitative Determination of Chlorpyrifos

Zalat

El-Sayed

Fayed

et al. 2013

ILCPA

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A specific and sensitive HPLC and UV spectrophotometric methodwere developed for determination and analysis of chlorpyrifos. Chromatographic separation was achieved on a 150 mm x 10 mm I.D. reversed phase column Zorbax SB C-18. usingdeionizedwater: acetonitrile in the ratio of 10:90 v/v respectively as mobile phase. The effluent was monitored at 290 and 230 nm. Two sharp peaks were obtained for the solvent and chlorpyrifos at 2.7 and 3.45 min respectively. UV spectrophotometric method was performed at 290 nm using Isopropanol as the solvent. Linear range was 0.025-3500 ppm (r2 = 0.9986 ±0.0009) for HPLC method and 2.229 to 200 ppm (r2 = 0.9988) for UV spectrophotometric method. Validation guidelines and statistical analysis showed that both the methods were precise, accurate, sensitive, and can be used for the routine quality control of chlorpyrifos in waste discharges

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