Validation of UV Spectrophotometric and HPLC Methods for Quantitative Determination of Chlorpyrifos

Abstract

A specific and sensitive HPLC and UV spectrophotometric methodwere developed for determination and analysis of chlorpyrifos. Chromatographic separation was achieved on a 150 mm x 10 mm I.D. reversed phase column Zorbax SB C-18. usingdeionizedwater: acetonitrile in the ratio of 10:90 v/v respectively as mobile phase. The effluent was monitored at 290 and 230 nm. Two sharp peaks were obtained for the solvent and chlorpyrifos at 2.7 and 3.45 min respectively. UV spectrophotometric method was performed at 290 nm using Isopropanol as the solvent. Linear range was 0.025-3500 ppm (r2 = 0.9986 ±0.0009) for HPLC method and 2.229 to 200 ppm (r2 = 0.9988) for UV spectrophotometric method. Validation guidelines and statistical analysis showed that both the methods were precise, accurate, sensitive, and can be used for the routine quality control of chlorpyrifos in waste discharges

Biodegradation of chlorpyrifos by soil bacteria and their effects on growth of rice seedlings under pesticide-contaminated soil

“…Growth determination and rapid detection of CP degradation in liquid medium. CP degradation was detected using the UV spectrophotometric method described by Zalat et al (2014). Briefly, 5% (v/v) overnight starter culture was inoculated in 50 mL of MSM.…”

Validation of HPLC Method for Quantitative Determination of Pirimiphos Methyl

“…Different chromatographic methods followed for the determination of Pirimiphos methyl in food and environmental samples [7][8]. For example, limits of detection (LOD) between 0.05 and 0.5 ng/ml were reached using thin layer chromatography (TLC) [9]; also high performance liquid chromatography (HPLC) with LOD of 16 ng/ml for the analysis of Pirimiphos methyl in water [10][11]. In addition, gas chromatography (GC) coupled to nitrogen/phosphorous (NPD) [12][13], atomic emission (AED) [14], electron capture (ECD) [15] or flame photometric detectors (FPD) [16] have also been applied for the determination of Pirimiphos methyl.…”