Thidiazuron-induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea): Endogenous growth regulator levels and significance of cotyledons

Murthy, B. N. S.; Murch, Susan J.; Saxena, Pulkit

doi:10.1034/j.1399-3054.1995.940213.x

Cited by 46 publications

(53 citation statements)

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“…Although TDZ application inhibits root elongation (Murthy et al 1995), we observed the thickening of roots during in vitro culture (Fig. 2b-d), a phenomenon not reported to date and related to a low survival of adventitious roots during acclimatization (Smith and McClelland 1991;Barry-Etienne et al 2002).…”

Section: Resultssupporting

confidence: 44%

“…Under our TDZ pulsing treatment, large and sometimes chlorotic leaves were noticed (Fig. 2b), the latter probably related to nutritional deficiencies (Deenik et al 2000) and possibly promoted by the use of the cytokinin, as observed by Murthy et al (1995).…”

Section: Resultsmentioning

confidence: 67%

“…Because TDZ is a highly stable compound and is resistant to oxidases (Mok et al 1987;Murthy et al 1995), it most likely caused residual effects on the tissues of the proximal region of the explants, inhibiting rooting when transferred to growth regulator-free medium (Figs. 1 and 2b).…”

Section: Resultsmentioning

confidence: 99%

“…Thidiazuron (TDZ) shows similar activity to cytokinins (Mok et al 1982;Mok and Mok 2001) and also induces auxin-like responses (Murthy et al 1995;Jones et al 2007). It enhances in vitro vegetative propagation in several species, both by the development of adventitious buds (Wilhelm 1999;Almeida et al 2012) and by the induction of somatic embryos (Hutchinson et al 1996;Zhang et al 2001;Almeida et al 2012).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

TDZ pulsing evaluation on the in vitro morphogenesis of peach palm

Graner

Oberschelp

Brondani

et al. 2013

Physiol Mol Biol Plants

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Peach palm (Bactris gasipaes Kunth.) cropping is an excellent alternative to native species exploitation; nevertheless, the problems with seed germination and conventional propagation justify the use of in vitro culturing. Aiming to asses TDZ pulsing effect on B. gasipaes morphogenesis, explants obtained from unarmed microplants were maintained in two treatments, half of them in MS free medium (without growth regulator) and the other half in MS with TDZ (0.36 μM). Both groups were transferred to growth regulator-free MS medium following 14 days of culture. After 84 days of culture, TDZ pulsing increased the growth and development of the shoots, restricted the growth and development of the roots, with no influence on adventitious bud induction or somatic embryogenesis. Furthermore, development of prickles, thickening of roots and chlorotic leaves were noted under TDZ pulsing. Leaf sheath histological analysis showed an epidermal origin and no vascularization of these prickles.

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Section: Resultssupporting

confidence: 44%

Section: Resultsmentioning

confidence: 67%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

TDZ pulsing evaluation on the in vitro morphogenesis of peach palm

Graner

Oberschelp

Brondani

et al. 2013

Physiol Mol Biol Plants

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“…Another possible reason for the higher activity of TDZ could be substitutive activity for both auxin and cytokinin required for shoot formation (Visser et al, 1992). Murthy et al (1995) and Hutchinson et al (1996) observed that endogenous auxin and cytokinins accumulated in tissues treated with TDZ. Indeed, CPPU has been shown to posses a stronger cytokininlike activity than BA in some species (Millian-Mendoza and Graham, 1999).…”

Section: Discussionmentioning

confidence: 99%

Effect of Urea-Type Cytokinins On The Adventitious Shoots Regeneration From Cotyledonary Node Explant in Thecommon Ice Plant,Mesembryanthemum Crystallinum

Sunagawa

Agarie

Umemoto

et al. 2007

Plant Production Science

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The multipotent thidiazuron: A mechanistic overview of its roles in callogenesis and other plant cultures in vitro

Ali

Khan

et al. 2022

Biotech and App Biochem

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Thidiazuron (TDZ) is an active substituted phenyl urea compound that has found a significant role as a plant growth regulator. The most exciting aspect of its function is that it can mimic auxins and cytokinin but is chemically different from these two. Many theories have been put forward, and experiments performed to understand the mode of action of TDZ in callogenesis. One suggested mechanism presents that it works by inhibiting the cytokinin degrading enzymes that compete with cytokinin for an active site on the enzyme. An example is the TDZ‐induced suppressed expression of gibberellic acid (GA) biosynthesis genes encoding GA3 and GA20 oxidases. This is entailed with a slightly increased expression of GA catabolism genes encoding GA20 oxidase. Similarly, one of the recommendations is that TDZ induces the expression of specific genes and transcription regulatory sequences that are either responsible directly for callus formation or in turn induce other auxins or cytokinin for callogenesis. There is no concise review available that discusses the details of TDZ‐induced callus, specifically and other in vitro cultures in general. This review is an attempt to explore all these pathways and mechanisms involved in callogenesis in plants stimulated by TDZ.

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Thidiazuron-induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea): Endogenous growth regulator levels and significance of cotyledons

Cited by 46 publications

References 0 publications

TDZ pulsing evaluation on the in vitro morphogenesis of peach palm

TDZ pulsing evaluation on the in vitro morphogenesis of peach palm

Effect of Urea-Type Cytokinins On The Adventitious Shoots Regeneration From Cotyledonary Node Explant in Thecommon Ice Plant,Mesembryanthemum Crystallinum

The multipotent thidiazuron: A mechanistic overview of its roles in callogenesis and other plant cultures in vitro

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