Thidiazuron: an efficient plant growth regulator for enhancing Agrobacterium-mediated transformation in Petunia hybrida

Thirukkumaran, Gunaratnam; Ntui, Valentine Otang; Khan, Raham Sher; Mii, Masahiro

doi:10.1007/s11240-009-9581-3

Cited by 18 publications

(9 citation statements)

References 33 publications

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“…Thidiazuron (TDZ) has been shown to enhance the frequency of shoot organogenesis in cotyledon explants (Vogel and Macedo 2011;Thirukkumaran et al 2009), which is in agreement with our findings as well. For P. pubescens, maintaining shoot regenerants on SEI medium containing TDZ for periods longer than 3-5 weeks resulted in the recovery of short and compact shoots (\1 cm in length, Fig.…”

Section: Regeneration Of Plants From Cotyledon Fragmentssupporting

confidence: 92%

Functional analysis of an iaaM gene in parthenocarpic fruit development in transgenic Physalis pubescens L. plants

Wang

Xin

Qin

et al. 2011

Plant Cell Tiss Organ Cult

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An efficient somatic embryogenesis system for Physalis pubescens L. (husk tomato) was developed prior to transformation. Subsequently, cotyledonary explants of P. pubescens were transformed with a chimeric construct containing an iaaM gene from driven by the fruit-specific promoter 2A12 to develop parthenocarpic fruits. Following selection of explants on Murashige and Skoog (MS) medium containing containing 75 mg l -1 kanamycin (Km), 36 km-resistant callus clusters were recovered, and these were regenerated into whole plants. Expression of the iaaM gene was detected in confirmed transgenic fruits. The 0.9-kb 2A12 promoter was capable of directing expression of the introduced iaaM gene in transgenic P. pubescens fruits, but iaaM expression was absent from both leaves and flowers. Quantitative measurements of indole-3-acetic acid (IAA) content during fruit development indicated that the IAA levels in transgenic lines increased from anthesis through young fruits and peaked at fruit maturity. On average, IAA contents in transgenic fruits were two-fold higher than those in control fruits. Under greenhouse condition, vegetative growth, morphology, and the flowering of transgenic plants were comparable to those of control plants. However, the fruits of transgenic lines ripened earlier and had fewer seeds per fruit than did control plants.

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Section: Regeneration Of Plants From Cotyledon Fragmentssupporting

confidence: 92%

Functional analysis of an iaaM gene in parthenocarpic fruit development in transgenic Physalis pubescens L. plants

Wang

Xin

Qin

et al. 2011

Plant Cell Tiss Organ Cult

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“…It is used to indicate transient and stable transformations (Serres et al 1992), and to estimate the relative contribution and strength of promoter elements in the transcriptional activation. Most recently, GUS histochemical assay was utilized in the production of markerfree Petunia hybrida and tomato (Khan et al , 2011, and in the production of a more efficient Agrobacterium tumefaciens-mediated transformation protocol in melon (Ntui et al 2010), in Lilium (Azadi et al 2010), in Petunia hybrida (Thirukkumaran et al 2009), in pineapple (Gangopadhyay et al 2009), in Piper colubrinum (Mani and Manjula 2011), in sesame (Yadav et al 2010), in…”

Section: Discussionmentioning

confidence: 99%

Characterization of inhibitor(s) of β-glucuronidase enzyme activity in GUS-transgenic wheat

Ramadan

Eissa

El-Domyati

et al. 2011

Plant Cell Tiss Organ Cult

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The uidA gene, encoding for b-glucuronidase (GUS), is the most frequently used reporter gene in plants.As a reporter enzyme, GUS can be assayed both qualitatively and quantitatively. In wheat, there are numerous reports of failure in detecting GUS enzyme activity in tissues of transgenic plants, while other reports have suggested presence of b-glucuronidase inhibitor(s) in wheat tissues. In the present study, we show that the b-glucuronidase enzyme activity is not only tissue-specific but also genotype-dependent. Our data demonstrate that the glucuronic acid could be the candidate inhibitor for b-glucuronidase enzyme activity in wheat leaves and roots. It should be noted that the assays to detect b-glucuronidase enzyme activity in wheat should be interpreted carefully. Based on the data of our present study, we recommend studying the chemical pathways, the unintended effects and the possible loss-of-function of any candidate transgene prior to transformation experiments.

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“…Previous studies reported a positive effect of Kin on regeneration of many other plant species such as Salix viminalis (Grendysz et al, 2017), and cumin (Tawfik and Noga, 2002). Many other studies have reported successful shoot regeneration from petunia leaf explants when cultured on medium containing TDZ alone (Abu-Qaoud, 2012, Thirukkumaran et al, 2009. They attributed that to the effect of TDZ when included in the medium resulting in a balanced ratio of endogenous PGR that allows for specific modes of regeneration to take place, which is likely dependent on concentration as well as the plant species.…”

Section: Shoot Organogenesis From Callus Explantsmentioning

confidence: 99%

Enhanced indirect organogenesis and efficient shoot multiplication of Petunia hybrida

Tawfik

Ibrahim

Taha

2019

Egyptian Journal of Horticulture

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A STUDY was initiated through various experiments aiming to optimize different stages including callus induction, shoot regeneration, shoot multiplication, rooting and acclimatization of Petunia hybrida. Callogenesis from leaves of in vitro-grown seedling was observed on MS medium supplemented with 0.1 mg/l NAA combined with BAP at various concentrations (0.0, 0.25, 0.5 and 1.0 mg/l). The results indicated that the medium with BAP at 0.5 mg/l in combination with NAA at 0.1 mg/l resulted in the highest percentage for explants producing callus (40%). Callus was transferred to regeneration medium to study the effect of three types of cytokinins including BAP, TDZ and Kin at different concentrations (0.0, 0.2, 0.4, 0.6, 0.8 and 1.0 mg/l). The results showed that BAP at 0.2 mg/l or TDZ at 0.2 or 0.4 mg/l induced the highest multiplication capacity (90%). Another experiment employed four MS basal salt strengths (full, ¾, ½ and ¼ MS) in combination with three concentrations of sucrose (20, 30 and 40 g/l). Full strength MS basal salt medium with sucrose (40 g/l) boosted multiplication performance. Multiplied shoots were studied for rooting ability. Applying sucrose at the lowest concentration (15 g/l) with full or ½ MS produced the highest rooting percent (100%), the highest number of roots (8.33) per shoot and the longest roots (12.03 cm). The well rooted plantlets were successfully acclimatized on different potting media including peat moss, vermiculite, clay and perlite where clay surpassed other growing medium mixtures with a 89% survival rate.

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Thidiazuron: an efficient plant growth regulator for enhancing Agrobacterium-mediated transformation in Petunia hybrida

Cited by 18 publications

References 33 publications

Functional analysis of an iaaM gene in parthenocarpic fruit development in transgenic Physalis pubescens L. plants

Functional analysis of an iaaM gene in parthenocarpic fruit development in transgenic Physalis pubescens L. plants

Characterization of inhibitor(s) of β-glucuronidase enzyme activity in GUS-transgenic wheat

Enhanced indirect organogenesis and efficient shoot multiplication of Petunia hybrida

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