Functional analysis of an iaaM gene in parthenocarpic fruit development in transgenic Physalis pubescens L. plants

Abstract

An efficient somatic embryogenesis system for Physalis pubescens L. (husk tomato) was developed prior to transformation. Subsequently, cotyledonary explants of P. pubescens were transformed with a chimeric construct containing an iaaM gene from driven by the fruit-specific promoter 2A12 to develop parthenocarpic fruits. Following selection of explants on Murashige and Skoog (MS) medium containing containing 75 mg l -1 kanamycin (Km), 36 km-resistant callus clusters were recovered, and these were regenerated into whole plants. Expression of the iaaM gene was detected in confirmed transgenic fruits. The 0.9-kb 2A12 promoter was capable of directing expression of the introduced iaaM gene in transgenic P. pubescens fruits, but iaaM expression was absent from both leaves and flowers. Quantitative measurements of indole-3-acetic acid (IAA) content during fruit development indicated that the IAA levels in transgenic lines increased from anthesis through young fruits and peaked at fruit maturity. On average, IAA contents in transgenic fruits were two-fold higher than those in control fruits. Under greenhouse condition, vegetative growth, morphology, and the flowering of transgenic plants were comparable to those of control plants. However, the fruits of transgenic lines ripened earlier and had fewer seeds per fruit than did control plants.

Development of plant regeneration and Agrobacterium tumefaciens-mediated transformation methodology for Physalis pruinosa

“…Development of the method reported here was based on our experience with transformation of other Solanaceae family members because although there are reports of plant regeneration and transformation of other Physalis species, we were unsuccessful with application of these methods to P. pruinosa (Otroshy et al 2013;Simpson et al 1995;Van Eck et al 2019;Wang et al 2011). Therefore, we chose to investigate the applicability of our tomato regeneration and transformation approaches and found these methods to be very effective for P. pruinosa (Van Eck et al 2019).…”

Development of plant regeneration andAgrobacterium tumefaciens-mediated transformation methodology forPhysalis pruinosa

“…Development of the method reported here was based on our experience with transformation of other Solanaceae family members because although there are reports of plant regeneration and transformation of other Physalis species, we were unsuccessful with application of these methods to P. pruinosa (Otroshy et al 2013, Simpson et al 1995, Wang et al 2011). As with tomato transformation methodology and reports on transformation of P. philadelphica, segments from cotyledons are the explant that works best for A. tumefaciensmediated transformation (Simpson et al 1995.…”

Ultrasonic-Assisted Extraction of Natural Yellow Pigment from Physalis pubescens L. and Its Antioxidant Activities

“…So Physalis pubescens L. has a nutrient, medicine, and economic importance in many countries. e fruits of Physalis are consumed either fresh or processed, mainly for jam, canning, or preserves [6], but less attention is paid to the rich natural yellow pigment resources in fruit. Natural yellow pigment is important for food industry, because natural pigment is much safer and healthier [7], so the demand for natural pigments is growing.…”