Thi1, a thiamine biosynthetic gene inArabidopsis thaliana, complements bacterial defects in DNA repair

Machado, Carlos Renato; Oliveira, Regina Costa de; Boiteux, Serge; Praekelt, Uta; Meacock, Peter A.; Menck, Carlos Frederico Martins

doi:10.1007/bf00042231

Cited by 101 publications

(104 citation statements)

References 47 publications

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“…This suggests that plants may utilize a biosynthetic pathway that is similar to that of microorganisms. Two genes involved in thiamine biosynthesis have so far been cloned and characterized in Arabidopsis [28][29][30]. The first one is THI1, which is involved in thiazole synthesis.…”

Section: Introductionmentioning

confidence: 99%

“…The first one is THI1, which is involved in thiazole synthesis. THI1 was isolated by complementation of the E.coli mutant strain BW535, a mutant with defective DNA base excision repair pathways [28]. Further characterization showed that THI1 has two functions: it is involved in the thiazole synthesis of the thiamine biosynthesis pathway and in DNA repair [28,29].…”

Section: Introductionmentioning

confidence: 99%

“…THI1 was isolated by complementation of the E.coli mutant strain BW535, a mutant with defective DNA base excision repair pathways [28]. Further characterization showed that THI1 has two functions: it is involved in the thiazole synthesis of the thiamine biosynthesis pathway and in DNA repair [28,29]. Also, THI1 possesses a signal peptide at its N terminus, which targets the THI1 protein to mitochondria and chloroplasts [31,32].…”

Section: Introductionmentioning

confidence: 99%

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AtTHIC, a gene involved in thiamine biosynthesis in Arabidopsis thaliana

Kong

Zhu

et al. 2008

Cell Res

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Thiamine (vitamin B1) is an essential compound for organisms. It contains a pyrimidine ring structure and a thiazole ring structure. These two moieties of thiamine are synthesized independently and then coupled together. Here we report the molecular characterization of AtTHIC, which is involved in thiamine biosynthesis in Arabidopsis. AtTHIC is similar to Escherichia coli ThiC, which is involved in pyrimidine biosynthesis in prokaryotes. Heterologous expression of AtTHIC could functionally complement the thiC knock-out mutant of E. coli. Downregulation of AtTHIC expression by T-DNA insertion at its promoter region resulted in a drastic reduction of thiamine content in plants and the knock-down mutant thic1 showed albino (white leaves) and lethal phenotypes under the normal culture conditions. The thic1 mutant could be rescued by supplementation of thiamine and its defect functions could be complemented by expression of AtTHIC cDNA. Transient expression analysis revealed that the AtTHIC protein targets plastids and chloroplasts. AtTHIC was strongly expressed in leaves, flowers and siliques and the transcription of AtTHIC was downregulated by extrinsic thiamine. In conclusion, AtTHIC is a gene involved in pyrimidine synthesis in the thiamine biosynthesis pathway of Arabidopsis, and our results provide some new clues for elucidating the pathway of thiamine biosynthesis in plants.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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AtTHIC, a gene involved in thiamine biosynthesis in Arabidopsis thaliana

Kong

Zhu

et al. 2008

Cell Res

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“…For example, the AtXPB (the letters At indicate A. thaliana) gene is homologous to the human XPB gene, which is involved in DNA repair and transcription (Ribeiro et al, 1998). The A. thaliana thi1 gene is homologous to the yeast thi4 gene and seems to be involved in thiamine biosynthesis and mitochondrial DNA damage tolerance (Machado et al, 1996;Machado et al, 1997). The AtMMH gene is homologous to the E. coli mutM gene, which encodes for a glycosylase that recognizes some types of oxidative deoxyguanosine dam-age (Ohtsubo et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Base excision repair in sugarcane

et al. 2001

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DNA damage can be induced by a large number of physical and chemical agents from the environment as well as compounds produced by cellular metabolism. This type of damage can interfere with cellular processes such as replication and transcription, resulting in cell death and/or mutations. The low frequency of mutagenesis in cells is due to the presence of enzymatic pathways which repair damaged DNA. Several DNA repair genes (mainly from bacteria, yeasts and mammals) have been cloned and their products characterized. The high conservation, especially in eukaryotes, of the majority of genes related to DNA repair argues for their importance in the maintenance of life on earth. In plants, our understanding of DNA repair pathways is still very poor, the first plant repair genes having only been cloned in 1997 and the mechanisms of their products have not yet been characterized. The objective of our data mining work was to identify genes related to the base excision repair (BER) pathway, which are present in the database of the Sugarcane Expressed Sequence Tag (SUCEST) Project. This search was performed by tblastn program. We identified sugarcane clusters homologous to the majority of BER proteins used in the analysis and a high degree of conservation was observed. The best results were obtained with BER proteins from Arabidopsis thaliana. For some sugarcane BER genes, the presence of more than one form of mRNA is possible, as shown by the occurrence of more than one homologous EST cluster.

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“…Em Arabidopis thaliana, o gene thi1 está relacionado com a via da biossíntese de tiamina, sendo composto por uma ORF de 1047 pares de bases, e codifica uma proteína de 349 resíduos de aminoácidos (9). A dedução da sequência polipeptídica da proteína THI1 revelou um alto grau de identidade com as proteínas envolvidas na biossíntese de tiamina em Saccharomyces cerevisiae (51,7% de identidade) e Schizosaccharomyces pombe (55,1% de identidade) (10).…”

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DUAS PROTEÍNAS (TcTIA6 e TcTIA7) ENVOLVIDAS NA BIOSSÍNTESE DA TIAMINA CODIFICADAS PELO GENOMA do Theobroma cacao L

Santana

Silva

Bernardes

2017

Infarma

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Thiamine functions as a cofactor for activity of various enzymes that act on the metabolism of carbohydrates and amino acids. This study aimed to analyze through bioinformatic techniques the genes responsible for the biosynthesis of thiamine encoded by the genome of Theobroma cacao L. We found two genes, one in chromosome 6 and one in chromosome 7, being appointed as TcTIA6 and TcTIA7. The multiple alignment revealed a strong identity with the Arabidopsis thaliana protein and its direction is destined to the secretory route of the chloroplast. Conserved motifs in the C-terminal region were found, indicating participation in the DNA repair mechanism. Analyzing the dendrogram it was evident that the genes studied had a common ancestor with Gossypium raimondii. The analysis showed that the TcTIA6 and TcTIA7 proteins are probably bifunctional, since they are involved in the biosynthesis of thiamine and stability of the DNA molecule.Key Words: cacao, computational biology, genome, protein, thiamine RESUMO A tiamina funciona como um cofator para atividades de várias enzimas que atuam no metabolismo de carboidratos e aminoácidos. Este trabalho objetivou analisar através de técnicas de bioinformática os genes responsáveis pela biossíntese da tiamina codificadas pelo genoma do Theobroma cacao L. Foram encontrados dois genes, um no cromossomo 6 e outro no 7, sendo nomeados TcTIA6 e TcTIA7. O alinhamento múltiplo da TcTIA6 e TcTIA7 revelou alta identidade com a proteína da Arabidopis thaliana e seu direcionamento é destinado para a rota secretora do cloroplasto. Foram observados motifs conservados na região C-terminal, indicando participação no mecanismo de reparo de DNA. Analisando o dendrograma ficou evidenciado que os genes estudados tiveram um ancestral comum com Gossypium raimondii. As análises mostraram que as proteínas TcTIA6 e TcTIA7 provavelmente são bifuncionais, uma vez que estão envolvidas na biossíntese da tiamina e na estabilidade da molécula de DNA.

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Thi1, a thiamine biosynthetic gene inArabidopsis thaliana, complements bacterial defects in DNA repair

Cited by 101 publications

References 47 publications

AtTHIC, a gene involved in thiamine biosynthesis in Arabidopsis thaliana

AtTHIC, a gene involved in thiamine biosynthesis in Arabidopsis thaliana

Base excision repair in sugarcane

DUAS PROTEÍNAS (TcTIA6 e TcTIA7) ENVOLVIDAS NA BIOSSÍNTESE DA TIAMINA CODIFICADAS PELO GENOMA do Theobroma cacao L

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