THI1, a protein involved in the biosynthesis of thiamin in Arabidopsis thaliana: Structural analysis of THI1(A140V) mutant

Garcia, Assuero F.; Dyszy, Fábio Henrique; Munte, Cláudia Elisabeth; DeMarco, Ricardo; Beltramini, Leila Maria; Oliva, Glaucius; Costa-Filho, Antonio J.; Araújo, Ana Paula Ulian de

doi:10.1016/j.bbapap.2014.03.005

Cited by 8 publications

(5 citation statements)

References 48 publications

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“…The thiazole moiety, 4-methyl-5-β-hydroxyethylthiazole phosphate (HET-P), on the other hand, is generated via the action of HET-P synthase ( THI1 , At5g54770 ; Godoi et al., 2006 ), which provides the required sulfur from a cysteine residue in the active site ( Sun et al., 2019 ). Hence, the THI1 enzyme undergoes only a single turnover and can therefore be considered suicidal ( Garcia et al., 2014 ). Subsequently, the bifunctional enzyme HMP-P kinase/TMP pyrophosphorylase ( TH1, At1g22940 ) phosphorylates HMP-P and condenses the arising product, HMP-pyrophosphate (HMP-PP), with HET-P, giving rise to TMP in the plastids ( Ajjawi et al., 2007b ).…”

Section: Introductionmentioning

confidence: 99%

Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants

et al. 2021

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Thiamin (or thiamine) is a water-soluble B-vitamin (B1), which is required, in the form of thiamin pyrophosphate (TPP), as an essential cofactor in crucial carbon metabolism reactions in all forms of life. To ensure adequate metabolic functioning, humans rely on a sufficient dietary supply of thiamin. Increasing thiamin levels in plants via metabolic engineering is a powerful strategy to alleviate vitamin B1 malnutrition and thus improve global human health. These engineering strategies rely on comprehensive knowledge of plant thiamin metabolism and its regulation. Here, multiple metabolic engineering strategies were examined in the model plant Arabidopsis thaliana. This was achieved by constitutive overexpression of the three biosynthesis genes responsible for B1 synthesis, HMP-P synthase (THIC), HET-P synthase (THI1) and HMP-P kinase/TMP pyrophosphorylase (TH1), either separate or in combination. By monitoring the levels of thiamin, its phosphorylated entities, and its biosynthetic intermediates, we gained insight into the effect of either strategy on thiamin biosynthesis. Moreover, expression analysis of thiamin biosynthesis genes showed the plant’s intriguing ability to respond to alterations in the pathway. Overall, we revealed the necessity to balance the pyrimidine and thiazole branches of thiamin biosynthesis and assessed its biosynthetic intermediates. Furthermore, the accumulation of non-phosphorylated intermediates demonstrated the inefficiency of endogenous thiamin salvage mechanisms. These results serve as guidelines in the development of novel thiamin metabolic engineering strategies.

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Section: Introductionmentioning

confidence: 99%

Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants

et al. 2021

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“…It comprises separate branches for the biosynthesis of the thiazole and pyrimidine moieties, by the action of THI1 and THIC, respectively, in the plastid (Fig. 1) [4][5][6][7] . Condensation of the two moieties is carried out by TH1 in the plastid to produce thiamine monophosphate (TMP) ( Fig.…”

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The coenzyme thiamine diphosphate displays a daily rhythm in the Arabidopsis nucleus

et al. 2020

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In plants, metabolic homeostasis-the driving force of growth and development-is achieved through the dynamic behavior of a network of enzymes, many of which depend on coenzymes for activity. The circadian clock is established to influence coordination of supply and demand of metabolites. Metabolic oscillations independent of the circadian clock, particularly at the subcellular level is unexplored. Here, we reveal a metabolic rhythm of the essential coenzyme thiamine diphosphate (TDP) in the Arabidopsis nucleus. We show there is temporal separation of the clock control of cellular biosynthesis and transport of TDP at the transcriptional level. Taking advantage of the sole reported riboswitch metabolite sensor in plants, we show that TDP oscillates in the nucleus. This oscillation is a function of a light-dark cycle and is independent of circadian clock control. The findings are important to understand plant fitness in terms of metabolite rhythms.

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“…As sequências primárias das proteínas do cacau apresentam motivos conservados na região C-terminal (Figura 1), que segundo alguns autores, são resíduos que indicam a participa-ção no mecanismo de reparo de DNA (10,12,23). Em outro estudo foi demostrado que esses genes em Citrus sinensis (laranja) também possuem atividades durante a maturação e a senescência dos frutos (24).…”

Section: Resultsunclassified

“…Em bactéria (Escherichia coli) este gene tem a capacidade de restabelecer o mecanismo de reparo em organismos que eram deficientes para esse tipo de reparo (10,12).…”

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DUAS PROTEÍNAS (TcTIA6 e TcTIA7) ENVOLVIDAS NA BIOSSÍNTESE DA TIAMINA CODIFICADAS PELO GENOMA do Theobroma cacao L

Santana

Silva

Bernardes

2017

Infarma

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Thiamine functions as a cofactor for activity of various enzymes that act on the metabolism of carbohydrates and amino acids. This study aimed to analyze through bioinformatic techniques the genes responsible for the biosynthesis of thiamine encoded by the genome of Theobroma cacao L. We found two genes, one in chromosome 6 and one in chromosome 7, being appointed as TcTIA6 and TcTIA7. The multiple alignment revealed a strong identity with the Arabidopsis thaliana protein and its direction is destined to the secretory route of the chloroplast. Conserved motifs in the C-terminal region were found, indicating participation in the DNA repair mechanism. Analyzing the dendrogram it was evident that the genes studied had a common ancestor with Gossypium raimondii. The analysis showed that the TcTIA6 and TcTIA7 proteins are probably bifunctional, since they are involved in the biosynthesis of thiamine and stability of the DNA molecule.Key Words: cacao, computational biology, genome, protein, thiamine RESUMO A tiamina funciona como um cofator para atividades de várias enzimas que atuam no metabolismo de carboidratos e aminoácidos. Este trabalho objetivou analisar através de técnicas de bioinformática os genes responsáveis pela biossíntese da tiamina codificadas pelo genoma do Theobroma cacao L. Foram encontrados dois genes, um no cromossomo 6 e outro no 7, sendo nomeados TcTIA6 e TcTIA7. O alinhamento múltiplo da TcTIA6 e TcTIA7 revelou alta identidade com a proteína da Arabidopis thaliana e seu direcionamento é destinado para a rota secretora do cloroplasto. Foram observados motifs conservados na região C-terminal, indicando participação no mecanismo de reparo de DNA. Analisando o dendrograma ficou evidenciado que os genes estudados tiveram um ancestral comum com Gossypium raimondii. As análises mostraram que as proteínas TcTIA6 e TcTIA7 provavelmente são bifuncionais, uma vez que estão envolvidas na biossíntese da tiamina e na estabilidade da molécula de DNA.

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THI1, a protein involved in the biosynthesis of thiamin in Arabidopsis thaliana: Structural analysis of THI1(A140V) mutant

Cited by 8 publications

References 48 publications

Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants

Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants

The coenzyme thiamine diphosphate displays a daily rhythm in the Arabidopsis nucleus

DUAS PROTEÍNAS (TcTIA6 e TcTIA7) ENVOLVIDAS NA BIOSSÍNTESE DA TIAMINA CODIFICADAS PELO GENOMA do Theobroma cacao L

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