Thermus thermophilus Strains Active in Purine Nucleoside Synthesis

Abstract

Several strains of Thermus thermophilus were tested in order to detect purine nucleoside synthase activity using pyrimidine nucleosides as the sugar-donor and adenine or hypoxanthine as bases. High productivity values (t =1 hr) were obtained while completely avoiding adenosine-deaminase degradation of the products. N-2-deoxy-ribosyltransferase activity is described for the first time in hyperthermophilic bacteria.

Thermus thermophilus Nucleoside Phosphorylases Active in the Synthesis of Nucleoside Analogues

“…It is interesting that the three enzymes favored the use of 2=-deoxyribosides rather than ribosides and that their specificity did not overlap, covering almost every combination of nucleobase and sugar, in agreement with our previous findings on cell extracts from this strain (2). Due to these results, we renamed TTC1070, TTC0194, and TTC1412 as TtPNPI, TtPNPII, and TtPyNP, respectively.…”

“…We reported previously the existence of nucleoside synthase activities in the whole cells of several T. thermophilus strains, including the sequenced strain HB27 (2). Here, we search for the genes encoding these activities in the genome of T. thermophilus HB27 (9) and identify three NP activities with a complementary set of specificities for substrates.…”

Applied Biotransformations in Green Solvents

“…Another alternative is whole‐cell trapping in different matrixes, such as porous polyacrylamide or polyacrylonitrile,61 calcium58 or barium alginate,62 or polyurethane foam,48 which act as a barrier against the toxic solvent. Finally, the use of hyperthermophiles (growing at T >80 °C) allows the possibility of employing useful enzymes that expand the range of the reaction conditions suitable for biocatalysis,19 especially in the presence of organic solvents.…”

“…In this way, some interesting enzymes (such as esterases, lipases, proteases, alcohol dehydrogenases, etc 16–18. or nucleoside phosphorylases) from Thermus thermophilus 19 have been described.…”

Purine and pyrimidine salvage pathway in thermophiles: a valuable source of biocatalysts for the industrial production of nucleic acid derivatives

“…In a very interesting study, Almendros et al (2009) tested several strains of T. thermophilus in their search for thermostable biocatalysts for the synthesis of ribo and 2′deoxyribonucleosides. As a result, T. thermophilus HB27, PRQ-16, PRQ-25, B, Fiji3A1, and VG7 were selected as the most relevant candidates.…”