Thermotaxis of human trophoblastic cells

Higazi, Abd Al‐Roof; Kniss, Douglas A.; Manuppello, J R; Barnathan, E. S.; Cines, Douglas B.

doi:10.1016/s0143-4004(96)80019-1

Cited by 17 publications

(21 citation statements)

References 12 publications

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“…Cells and lower organisms commonly employ thermotaxis for migrating to preferable temperatures. Thermotaxis was first discovered in the unicellular slime mold Dictyostelium discoideum (Bonner et al, 1950;Raper, 1940) and then, among others, in Caenorhabditis elegans (Hedgecock and Russell, 1975), bacteria (Maeda et al, 1976), parasites like Dirofilaria immitis larvae (Mok et al, 1986), Drosophila (Sayeed and Benzer, 1996), human polymorphonuclear leukocytes (Kessler et al, 1979;Mizuno et al, 1992), human trophoblastic cells (Higazi et al, 1996), and mammalian spermatozoa (Bahat et al, 2003). In some systems [e.g., bacteria (Maeda et al, 1976), D .…”

Section: Sperm Thermotaxis In Comparison To Other Systemsmentioning

confidence: 99%

Sperm thermotaxis

Bahat

Eisenbach

2006

Molecular and Cellular Endocrinology

114

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Thermotaxis--movement directed by a temperature gradient--is a prevalent process, found from bacteria to human cells. In the case of mammalian sperm, thermotaxis appears to be an essential mechanism guiding spermatozoa, released from the cooler reservoir site, towards the warmer fertilization site. Only capacitated spermatozoa are thermotactically responsive. Thermotaxis appears to be a long-range guidance mechanism, additional to chemotaxis, which seems to be short-range and likely occurs at close proximity to the oocyte and within the cumulus mass. Both mechanisms probably have a similar function--to guide capacitated, ready-to-fertilize spermatozoa towards the oocyte. The temperature difference between the site of the sperm reservoir and the fertilization site is generated at ovulation by a temperature drop at the former. The molecular mechanism of sperm thermotaxis waits to be revealed.

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Section: Sperm Thermotaxis In Comparison To Other Systemsmentioning

confidence: 99%

Sperm thermotaxis

Bahat

Eisenbach

2006

Molecular and Cellular Endocrinology

114

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“…Planck's law relates the spectral emissive power of a black body to its temperature through the following: (2) where is the wavelength in , is absolute temperature, and and are the first and second radiation constants, respectively. Real materials are not black bodies, but within narrow wavelength and temperature ranges can be considered "grey bodies" wherein the above equation holds true if the emissivity value of a material is considered.…”

Section: B Infrared Microscopymentioning

confidence: 99%

“…Corresponding intensity and temperature plots are given in Fig. 5, where temperature was related to intensity through (2). All data were passed through a smoothing algorithm to help filter noise.…”

Section: B Infrared Microscopymentioning

confidence: 99%

Microheated substrates for patterning cells and controlling development

Shu

Everett

Zhang

et al. 2005

J. Microelectromech. Syst.

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Abstract-Here, we seek to control cellular development by devising a means through which cells can be subjected to a microheated environment in standard culture conditions. Numerous techniques have been devised for controlling cellular function and development via manipulation of surface environmental cues at the micro-and nanoscale. It is well understood that temperature plays a significant role in the rate of cellular activities, migratory behavior (thermotaxis), and in some cases, protein expression. Yet, the effects and possible utilization of micrometer-scale temperature fields in cell cultures have not been explored. Toward this end, two types of thermally isolated microheated substrates were designed and fabricated, one with standard backside etching beneath a dielectric film and another with a combination of surface and bulk micromachining and backside etching. The substrates were characterized with infrared microscopy, finite element modeling, scanning electron microscopy, stylus profilometry, and electrothermal calibrations. Neuron culture studies were conducted on these substrates to 1) examine the feasibility of using a microheated environment to achieve patterned cell growth and 2) selectively accelerate neural development on regions less than 100 m wide. Results show that attached neurons, grown on microheated regions set at 37 C, extended processes substantially faster than those incubated at 25 C on the same substrate. Further, unattached neurons were positioned precisely along the length of the heater filament (operating at 45 C) using free convection currents. These preliminary findings indicate that microheated substrates may be used to direct cellular development spatially in a practical manner.[1414]

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“…In addition to mechanotaxis, gradient of chemical substance or temperature in the substrate gives rise to chemotactic [ 16 , 17 ] or thermotactic [ 18 , 19 ] cell shape changes during migration, respectively. Existent chemical and thermal gradients in the substrate regulate the direction of pseudopods in such a way that the cell migrates in the direction of the most effective cues [ 19 , 20 ]. However, it is actually myosin-based traction force (a mechanotactic tool) that provides the force driving the cell body forward [ 12 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, a majority of authors have experimentally considered cell movement in the presence of chemotactic cue [ 17 , 20 ] demonstrating that a shallow chemoattractant gradient guides the cell in the direction of imposed chemical gradient such that the extended pseudopods and cell elongation are turned in the direction of the gradient [ 20 ]. In contrast, some cells such as human trophoblasts subjected to oxygen and thermal gradients do not migrate in response to oxygen gradient (a chemotactic cue) but they elongate and migrate in response to thermal gradients of even less than 1°C towards the warmer locations [ 19 ]. However, there are some other cases such as burn traumas, influenza or some wild cell types that cell may migrate towards the lower temperature, away from warm regions [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Numerical Model of Cell Morphology during Migration in Multi-Signaling Substrates

Mousavi

Doweidar

2015

PLoS ONE

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Cell Migration associated with cell shape changes are of central importance in many biological processes ranging from morphogenesis to metastatic cancer cells. Cell movement is a result of cyclic changes of cell morphology due to effective forces on cell body, leading to periodic fluctuations of the cell length and cell membrane area. It is well-known that the cell can be guided by different effective stimuli such as mechanotaxis, thermotaxis, chemotaxis and/or electrotaxis. Regulation of intracellular mechanics and cell’s physical interaction with its substrate rely on control of cell shape during cell migration. In this notion, it is essential to understand how each natural or external stimulus may affect the cell behavior. Therefore, a three-dimensional (3D) computational model is here developed to analyze a free mode of cell shape changes during migration in a multi-signaling micro-environment. This model is based on previous models that are presented by the same authors to study cell migration with a constant spherical cell shape in a multi-signaling substrates and mechanotaxis effect on cell morphology. Using the finite element discrete methodology, the cell is represented by a group of finite elements. The cell motion is modeled by equilibrium of effective forces on cell body such as traction, protrusion, electrostatic and drag forces, where the cell traction force is a function of the cell internal deformations. To study cell behavior in the presence of different stimuli, the model has been employed in different numerical cases. Our findings, which are qualitatively consistent with well-known related experimental observations, indicate that adding a new stimulus to the cell substrate pushes the cell to migrate more directionally in more elongated form towards the more effective stimuli. For instance, the presence of thermotaxis, chemotaxis and electrotaxis can further move the cell centroid towards the corresponding stimulus, respectively, diminishing the mechanotaxis effect. Besides, the stronger stimulus imposes a greater cell elongation and more cell membrane area. The present model not only provides new insights into cell morphology in a multi-signaling micro-environment but also enables us to investigate in more precise way the cell migration in the presence of different stimuli.

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Thermotaxis of human trophoblastic cells

Cited by 17 publications

References 12 publications

Sperm thermotaxis

Sperm thermotaxis

Microheated substrates for patterning cells and controlling development

Three-Dimensional Numerical Model of Cell Morphology during Migration in Multi-Signaling Substrates

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