Thermostablility of phycobiliproteins and antioxidant activity from four thermotolerant cyanobacteria

Pumas, Chayakorn; Vacharapiyasophon, Panmuk; Peerapornpisal, Yuwadee; Leelapornpisid, Pimporn; Boonchum, Walailuck; Ishii, Masaharu; Khanongnuch, Chartchai

doi:10.1111/j.1440-1835.2011.00615.x

Cited by 54 publications

(22 citation statements)

References 42 publications

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“…It is worth noting that the latter experiments were carried out on PBPs devoid of linker polypeptides, a lack that may somewhat modulate the thermotolerance of these molecules. Yet, the mid-unfolding temperature values reported here are quite in the range of previously reported values for other PBPs (Edwards et al, 1997;Patel et al, 2004;Pumas et al, 2011;Chaiklahan et al, 2012;Pumas et al, 2012).…”

Section: Resultssupporting

confidence: 80%

Adaptive thermostability of light-harvesting complexes in marine picocyanobacteria

2016

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Marine Synechococcus play a key role in global oceanic primary productivity. Their wide latitudinal distribution has been attributed to the occurrence of lineages adapted to distinct thermal niches, but the physiological and molecular bases of this ecotypic differentiation remain largely unknown. By comparing six strains isolated from different latitudes, we showed that the thermostability of their light-harvesting complexes, called phycobilisomes (PBS), varied according to the average sea surface temperature at strain isolation site. Comparative analyses of thermal unfolding curves of the three phycobiliproteins (PBP) constituting PBS rods suggested that the differences in thermostability observed on whole PBSs relied on the distinct molecular flexibility and stability of their individual components. Phycocyanin was the least thermostable of all rod PBP, constituting a fragility point of the PBS under heat stress. Amino-acid composition analyses and structural homology modeling notably revealed the occurrence of two amino-acid substitutions, which might have a role in the observed differential thermotolerance of this phycobiliprotein among temperature ecotypes. We hypothesize that marine Synechococcus ancestors occurred first in warm niches and that during the colonization of cold, high latitude thermal niches, their descendants have increased the molecular flexibility of PBP to maintain optimal light absorption capacities, this phenomenon likely resulting in a decreased stability of these proteins. This apparent thermoadaptability of marine Synechococcus has most probably contributed to the remarkable ubiquity of these picocyanobacteria in the ocean.

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Section: Resultssupporting

confidence: 80%

Adaptive thermostability of light-harvesting complexes in marine picocyanobacteria

2016

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“…PD40‐1 (5.43 ± 0.01 mg/g) (Pumas et al. ). A study carried out by Aydas et al., reported the TPC value in Synechocystis sp.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant properties in some selected cyanobacteria isolated from fresh water bodies of Sri Lanka

Hossain

Ratnayake

Meerajini

et al. 2016

Food Science & Nutrition

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Phytonutrients and pigments present in cyanobacteria act as antioxidants, which facilitate the formation of body's defense mechanism against free radical damage to cells. The aim of this investigation was to study the total phenolic content ( TPC ), total flavonoid content ( TFC ), antioxidant activity, phycobiliproteins ( PBP s), and active compounds in four cyanobacterial species, that is, Oscillatoria sp., Lyngbya sp., Microcystis sp., and Spirulina sp. isolated from fresh water bodies of Sri Lanka. In this study, Lyngbya sp. , showed highest TPC (5.02 ± 0.20 mg/g), TFC (664.07 ± 19.76 mg/g), and total PBP s (127.01 mg/g) value. The ferric reducing antioxidant power ( FRAP ) was recorded highest in Oscillatoria sp. (39.63 ± 7.02), whereas the 2,2‐diphenyl‐1‐picrylhydrazyl ( DPPH ) radical scavenging activity was also reported the highest in Oscillatoria sp. (465.31 ± 25.76) followed by Lyngbya sp. (248.39 ± 11.97). In FTIR spectroscopy, Lyngbya sp. does not show any N‐H stretching band which is ultimately responsible for the inhibition of antioxidant activity. The study revealed that Lyngbya sp. and Oscillatoria sp. can be an excellent source for food, pharmaceutical, and other industrial uses.

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“…Due to these characteristics, Oscillatoria sp. trend to occur more often in reservoirs, lakes, ponds and dams (Pumas et al, 2011). The Oscillatoria sp.…”

Section: Introductionmentioning

confidence: 99%

Impact of Chemical Coagulants for Oscillatoria sp. Removal from Raw Water on Chemical Coagulation Process

Thammasane¹,

Kaosol²

2018

American Journal of Environmental Sciences

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Growing Oscillatoria sp. in freshwater causes several poisoning episodes of domestic livestock animal and human. The chemical coagulation of raw water using chemical coagulants is very simple and requiring less investment cost on equipment. These experiments used alum and PACl (Polyaluminium chloride) with alum as chemical coagulants for Oscillatoria sp. removal. The Oscillatoria sp. was prepared from Molecular and Cell Laboratory of Thailand Institute of Scientific and Technological Research and grown in BG-11 medium. The samples of treated water use chemical coagulation test to verify their efficiency of Oscillatoria sp. cells removal from the synthetic raw water. The results show that the chemical coagulation using alum coagulant and PACl with alum coagulants produces a high efficiency in Oscillatoria sp. removal. However, the PACl with alum coagulants provided the best Oscillatoria sp. removal efficiency and the low amount of chemical coagulants for Oscillatoria sp. removal process. The PACl with alum doses at 1:10 by weight, provides the best Oscillatoria sp. removal efficiency at 97%. While, the best Oscillatoria sp. removal efficiency of alum dose at 90 mg/L, is 98%. It can be concluded that the PACl with alum doses as the combined chemical coagulant provided the highest efficiency at the lowest amount of chemical coagulants used.

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Thermostablility of phycobiliproteins and antioxidant activity from four thermotolerant cyanobacteria

Cited by 54 publications

References 42 publications

Adaptive thermostability of light-harvesting complexes in marine picocyanobacteria

Adaptive thermostability of light-harvesting complexes in marine picocyanobacteria

Antioxidant properties in some selected cyanobacteria isolated from fresh water bodies of Sri Lanka

Impact of Chemical Coagulants for Oscillatoria sp. Removal from Raw Water on Chemical Coagulation Process

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