Thermoresponsive Chitosan/DOPA-Based Hydrogel as an Injectable Therapy Approach for Tissue-Adhesion and Hemostasis

Abstract

Chitosan (CS) hydrogels are widely used in wound hemostatic agents due to their superior biocompatibility, biodegradability, and hemostatic effect. However, most of them fail to achieve great hemostatic effect because of poor adhesion to bleeding tissues. Also, the conventional implantation surgery of hemostatic hydrogels to internal bleeding wounds may cause secondary trauma to the human body. In this work, catechol-hydroxybutyl chitosan (HBCS-C) has been designed and prepared by grafting hydroxybutyl groups and catechol groups to the CS backbones. The multifunctional HBCS-C hydrogels are fabricated with the properties of thermosensitivity, injectability, tissue-adhesion, biodegradation, biocompatibility, and wound hemostasis. They exhibit excellent liquid-gel transition at different temperatures, through the changes of hydrophilic−hydrophobic interaction and hydrogen bonds generating from hydroxybutyl groups. By the multiple interactions between catechol groups/amino groups and tissues, the biocompatible hydrogels can strongly adhere on the surface of tissue. To further study, the bleeding rat-liver models are made to evaluate the hemostatic effects. After injecting the hydrogel precursor solution into the rat body, the hydrogels are not only formed in situ within 30 s but are also firmly adhered to the bleeding tissues which shows effective hemostasis. The injectability and tissue-adhesion improvement in this study gives a new insight into hemostatic agents, and the multifunctional hydrogels have a great potential in the biomedical application.

Regeneration of skeletal system with genipin crosslinked biomaterials

“…The positive charge in the chitosan triggered platelet activation, adhesion, and aggregation, promoting an initial hemostatic process. 114 The genipin then crosslinked with the fibrinogen in the blood to form fibrin clots, which further reduced blood loss; and 5% genipin exhibited the optimum coagulant activity. 70 Another noticeable issue is the infection of open fractures, as bacteria can come into direct contacting with and colonizing the inner bone tissue.…”

Recent advances on polymeric hydrogels as wound dressings

“…In the work reported by Shou et al , 66 they prepared catechol-hydroxybutyl chitosan (HBCS-C) hydrogel, with the properties of hemostasis, thermo-sensitivity, injectable capability, tissue-adhesion, biodegradation, and biocompatibility, via hydrophobic interactions and hydrogen bonding ( Fig. 4 ).…”

Marine Polysaccharides for Wound Dressings Application: An Overview

“…This effect is mainly achieved by enhancing the adhesion of the MPs dressing. Adhesive dressings are applied tightly to the wound with compression to promote clotting, exemplified by the mussel-inspired technology [288][289][290]. In recent years, with the development of smart hydrogel technology, injectable thermosensitive MPs hydrogels have been widely explored for in vivo wound haemostasis applications.…”