Thermoplasmonic-Assisted Cyclic Cleavage Amplification for Self-Validating Plasmonic Detection of SARS-CoV-2

Qiu, Guangyu; Gai, Zhibo; Saleh, Lanja; Tang, Jiukai; Gui, Ting; Kullak‐Ublick, Gerd A.; Wang, Jing

doi:10.1021/acsnano.1c00957

Cited by 50 publications

(73 citation statements)

References 49 publications

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“…However, the detection strategy involving the PPT effect of this biosensor is only suitable for the detection of nucleic acid, and not protein or enzyme, as the photothermal effect can literally denature the protein molecules [93]. This work further extends the utility of the photothermal-assisted plasmonic sensing biosensing system with the introduction of the thermoplasmonic-assisted dual-mode transducing (TP-DMT) concept [94]. The TP-DMT involves two types of approaches, which are an amplification-free-based direct viral RNA detection (direct sensing system) and an amplification-based cyclic fluorescence probe cleavage (CFPC) detection.…”

Section: Localized Surface Plasmon Resonancementioning

confidence: 91%

“…This work further extends the utility of the photothermal-assisted plasmonic sensing biosensing system with the introduction of the thermoplasmonic-assisted dual-mode transducing (TP-DMT) concept [ 94 ]. The TP-DMT involves two types of approaches, which are an amplification-free-based direct viral RNA detection (direct sensing system) and an amplification-based cyclic fluorescence probe cleavage (CFPC) detection.…”

Section: The Development Of Optical and Electrochemical Metal Nanoparticles-based Biosensors For The Detection Of Viral Rnamentioning

confidence: 98%

“… Schematic of TP-DMT viral sensing workflow with two types of approaches, which are the amplification-free-based direct viral RNA detection (direct sensing system) and the amplification-based cyclic fluorescence probe cleavage (CFPC) detection. Reproduced from Qiu et al [ 94 ]. …”

Section: Figurementioning

confidence: 99%

See 2 more Smart Citations

A Review on the Development of Gold and Silver Nanoparticles-Based Biosensor as a Detection Strategy of Emerging and Pathogenic RNA Virus

Ibrahim

Jamaluddin

Tan

et al. 2021

Sensors

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The emergence of highly pathogenic and deadly human coronaviruses, namely SARS-CoV and MERS-CoV within the past two decades and currently SARS-CoV-2, have resulted in millions of human death across the world. In addition, other human viral diseases, such as mosquito borne-viral diseases and blood-borne viruses, also contribute to a higher risk of death in severe cases. To date, there is no specific drug or medicine available to cure these human viral diseases. Therefore, the early and rapid detection without compromising the test accuracy is required in order to provide a suitable treatment for the containment of the diseases. Recently, nanomaterials-based biosensors have attracted enormous interest due to their biological activities and unique sensing properties, which enable the detection of analytes such as nucleic acid (DNA or RNA), aptamers, and proteins in clinical samples. In addition, the advances of nanotechnologies also enable the development of miniaturized detection systems for point-of-care (POC) biosensors, which could be a new strategy for detecting human viral diseases. The detection of virus-specific genes by using single-stranded DNA (ssDNA) probes has become a particular interest due to their higher sensitivity and specificity compared to immunological methods based on antibody or antigen for early diagnosis of viral infection. Hence, this review has been developed to provide an overview of the current development of nanoparticles-based biosensors that target pathogenic RNA viruses, toward a robust and effective detection strategy of the existing or newly emerging human viral diseases such as SARS-CoV-2. This review emphasizes the nanoparticles-based biosensors developed using noble metals such as gold (Au) and silver (Ag) by virtue of their powerful characteristics as a signal amplifier or enhancer in the detection of nucleic acid. In addition, this review provides a broad knowledge with respect to several analytical methods involved in the development of nanoparticles-based biosensors for the detection of viral nucleic acid using both optical and electrochemical techniques.

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Section: Localized Surface Plasmon Resonancementioning

confidence: 91%

Section: The Development Of Optical and Electrochemical Metal Nanoparticles-based Biosensors For The Detection Of Viral Rnamentioning

confidence: 98%

See 1 more Smart Citation

A Review on the Development of Gold and Silver Nanoparticles-Based Biosensor as a Detection Strategy of Emerging and Pathogenic RNA Virus

Ibrahim

Jamaluddin

Tan

et al. 2021

Sensors

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“…Finally, the extracted biomacromolecules were analyzed for quantitative detection of bioaerosols [ 94 ]. A thermoplasmonic-assisted dual-mode transducer was presented to detect the SARS-CoV-2 viruses in 30 min, where an amplification-free-based direct viral RNA detection and an amplification-based cyclic fluorescence probe cleavage detection were combined and demonstrated as a potential tool for fast clinical infection screening and real-time environmental monitoring [ 95 ]. Besides, some other molecular biological detection methods were also applied to detect the SARS-CoV-2 viruses [ 96 , 97 ].…”

Section: Pathogen Detectionmentioning

confidence: 99%

Challenges and Perspectives for Biosensing of Bioaerosol Containing Pathogenic Microorganisms

Lei

et al. 2021

Micromachines

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As an important route for disease transmission, bioaerosols have received increasing attention. In the past decades, many efforts were made to facilitate the development of bioaerosol monitoring; however, there are still some important challenges in bioaerosol collection and detection. Thus, recent advances in bioaerosol collection (such as sedimentation, filtration, centrifugation, impaction, impingement, and microfluidics) and detection methods (such as culture, molecular biological assay, and immunological assay) were summarized in this review. Besides, the important challenges and perspectives for bioaerosol biosensing were also discussed.

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“…Qiu et al [ 70 ] have reported the application of a nanoplatform including AuNI plasmonic chip for the quantitative measurement of SARS-CoV-2 within 30 min. They have suggested a novel concept based on the amplification-free-based direct viral RNA detection and an amplification-based cyclic fluorescence probe cleavage (CFPC) enabling the tests of clinical COVID-19 patient samples.…”

Section: Coronavirusesmentioning

confidence: 99%

Role of plasmonics in detection of deadliest viruses: a review

et al. 2021

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Viruses have threatened animal and human lives since a long time ago all over the world. Some of these tiny particles have caused disastrous pandemics that killed a large number of people with subsequent economic downturns. In addition, the quarantine situation itself encounters the challenges like the deficiency in the online educational system, psychiatric problems and poor international relations. Although viruses have a rather simple protein structure, they have structural heterogeneity with a high tendency to mutation that impedes their study. On top of the breadth of such worldwide worrying issues, there are profound scientific gaps, and several unanswered questions, like lack of vaccines or antivirals to combat these pathogens. Various detection techniques like the nucleic acid test, immunoassay, and microscopy have been developed; however, there is a tradeoff between their advantages and disadvantages like safety in sample collecting, invasiveness, sensitivity, response time, etc. One of the highly resolved techniques that can provide early-stage detection with fast experiment duration is plasmonics. This optical technique has the capability to detect viral proteins and genomes at the early stage via highly sensitive interaction between the biological target and the plasmonic chip. The efficiency of this technique could be proved using commercialized techniques like reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) techniques. In this study, we aim to review the role of plasmonic technique in the detection of 11 deadliest viruses besides 2 common genital viruses for the human being. This is a rapidly moving topic of research, and a review article that encompasses the current findings may be useful for guiding strategies to deal with the pandemics . By investigating the potential aspects of this technique, we hope that this study could open new avenues toward the application of point-of-care techniques for virus detection at early stage that may inhibit the progressively hygienic threats.

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Thermoplasmonic-Assisted Cyclic Cleavage Amplification for Self-Validating Plasmonic Detection of SARS-CoV-2

Cited by 50 publications

References 49 publications

A Review on the Development of Gold and Silver Nanoparticles-Based Biosensor as a Detection Strategy of Emerging and Pathogenic RNA Virus

A Review on the Development of Gold and Silver Nanoparticles-Based Biosensor as a Detection Strategy of Emerging and Pathogenic RNA Virus

Challenges and Perspectives for Biosensing of Bioaerosol Containing Pathogenic Microorganisms

Role of plasmonics in detection of deadliest viruses: a review

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