Challenges and Perspectives for Biosensing of Bioaerosol Containing Pathogenic Microorganisms

Abstract

As an important route for disease transmission, bioaerosols have received increasing attention. In the past decades, many efforts were made to facilitate the development of bioaerosol monitoring; however, there are still some important challenges in bioaerosol collection and detection. Thus, recent advances in bioaerosol collection (such as sedimentation, filtration, centrifugation, impaction, impingement, and microfluidics) and detection methods (such as culture, molecular biological assay, and immunological assay) were summarized in this review. Besides, the important challenges and perspectives for bioaerosol biosensing were also discussed.

Performance Evaluation and Validation of Air Samplers To Detect Aerosolized Coxiella burnetii

“…The AirPort MD8 was more user friendly in aspects important for operation in the field than the liquid impingers. This device uses a GMF that has shown higher collection efficiency than other types of filters ( 48 ) due to its layered structure, decreasing the chance of particles passing through. The AirPort MD8 also has moderate airflow rates that enable air sampling to be achieved within a reasonable time.…”

Paper-based sensors for bacteria detection

“…Because of the nature of the sample, assays typically consist of two main steps: collection and detection. Collection can be achieved by sedimentation, filtration, centrifugation, impaction, impingement or microfluidic chips 154 . For example, air samples with airborne bacteria can be passed through a porous sampling pad on a paper-based device 155 , 156 .…”

Atmospheric dispersion of chemical, biological, and radiological hazardous pollutants: Informing risk assessment for public safety

“…A lowcost, one-step, sensitive, and accurate multiplex detection electrochemical biochip has recently been developed to detect the spike, nucleocapsid proteins, and IgG antibodies of SARS-CoV-2 [214] , which can be easily modified to specifically detect different types of bioaerosols, for example, ARGs [215] . However, there are still many challenges in bioaerosol detection [216] .…”