Thermophilic production of polyhydroxyalkanoates by a novel<i>Aneurinibacillus</i>strain isolated from Gudao oilfield, China

Xiao, Zijun; Zhang, Yu; Xi, Lijun; Huo, Fangfang; Zhao, Jingyi; Li, Jing

doi:10.1002/jobm.201400843

Cited by 34 publications

(11 citation statements)

References 38 publications

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“…A further screening of thermophilic, contamination-resistant PHA producers was carried out by Xiao et al [111]. During this study, the strain XH2, isolated from an oilfield, was characterized from a morphological, physiological, and biochemical perspective, and, supported by 16S rDNA analysis, classified as Aneurinibacillus sp.…”

Section: Thermophile Pha Producersmentioning

confidence: 99%

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Koller¹

2017

MOJPS

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The article reviews the current state of knowledge of the production of polyhydroxyalkanoate (PHA) biopolyesters under extreme environmental conditions.Although PHA production by extremophiles is not realized yet at industrial scale, significant PHA accumulation under high salinity or extreme pH-or temperature conditions was reported for diverse representatives of the microbial domains of both Archaea and Bacteria. Several mechanisms were proposed to explain the mechanistic role of PHA and their monomers as microbial cell-and enzyme protective chaperons and the factors boosting PHA biosynthesis under environmental stress conditions. The potential of selected extremophile strains, isolated from extreme environments like glaciers, hot springs, saline brines, or from habitats highly polluted with heavy metals or solvents, for efficient future PHA production on an industrially relevant scale is assessed based on the basic data available in the scientific literature. The article reveals that, beside the needed optimization of other cost-decisive factors like inexpensive raw materials or efficient downstream processing, the application of extremophile production strains can drastically safe energy costs, are easily accessible towards long-term cultivation in chemostat processes, and therefore might pave the way towards cost-efficient PHA production, even combined with safe disposal of industrial waste streams. However, further challenges have still to be overcome in terms of strain improvement and process engineering aspects.

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Section: Thermophile Pha Producersmentioning

confidence: 99%

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Koller¹

2017

MOJPS

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“…The partial 16S rRNA gene sequences of the purified isolates were amplified using universal primers 27F and 1492R, and were sequenced using an ABI 3730XL DNA Analyser (Applied Biosystems) by Suzhou Genewiz Bio‐Tech Co., Ltd (China). The resultant sequences were compared with those of type species deposited in EzTaxon .…”

Section: Methodsmentioning

confidence: 99%

Non‐sterilized fermentative production of acetoin with 2,3‐butanediol as a main byproduct from maize hydrolysate by a newly isolated thermophilic Bacillus strain

Xiao¹,

Gu²,

Hou³

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND Acetoin is an important biorefinery platform chemical with many industrial uses. It is mainly produced by chemical synthesis or mesophilic fermentation with high costs. RESULTS A thermophilic Bacillus strain was isolated to efficiently produce acetoin from maize hydrolysate at 50°C. The fermentation medium was statistically optimized by Plackett–Burman design. Operational conditions were then optimized using a 4.2 L fermenter conducted at 50°C without sterilization. Oxygen enriched air (65% oxygen) accelerated substrate consumption but did not increase product titer. In comparison with 600 rpm, agitation speed controlled at 400 rpm yielded less acetoin but more 2,3‐butanediol in the final products. A two‐stage pH control strategy (pH first at 6.6–7.1 then at 7.7–8.0) was applied to further increase the yield of acetoin. Finally, 50.8 g L‐1 of acetoin and 32.1 g L‐1 of 2,3‐butanediol were obtained from 251 g L‐1 of degermed maize flour (dry weight). The conversion yield from sugar to the 4‐carbon compounds (acetoin and 2,3‐butanediol) was 94.4% of the theoretical value. CONCLUSION This is the first report of non‐sterilized fermentative production of acetoin. Using degermed maize hydrolysate as the main feedstock, this work sets a new titer record of acetoin fermentation among known thermophilic producing systems. © 2017 Society of Chemical Industry

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“…So far, more and more novel bacteria with the capable of enhanced production of PHA or better adaption to the fermentation processes have been isolated, and can be used as the best candidate for the PHA biosynthesis (Xiao et al . ). Recombinant E. coli harbouring the PHA synthetic pathway was also frequently used for biopolymer production for its many advantages such as high level of productivity, high cell density fermentation, etc.…”

Section: The Optimized Strategies Used In the Production Of Phasmentioning

confidence: 97%

“…Lu and his coworkers have metabolically engineered a mutant strain (Ralstonia eutropha W50) expressing the genes encoding L-arabinose catabolic enzymes, and therefore can use L-arabinose as the carbon feedstock to accumulate a noticeable level of PHB (Lu et al 2013). So far, more and more novel bacteria with the capable of enhanced production of PHA or better adaption to the fermentation processes have been isolated, and can be used as the best candidate for the PHA biosynthesis (Xiao et al 2015). Recombinant E. coli harbouring the PHA synthetic pathway was also frequently used for biopolymer production for its many advantages such as high level of productivity, high cell density fermentation, etc.…”

Section: Supplying Renewable Carbon Substratesmentioning

confidence: 99%

Recent strategies for efficient production of polyhydroxyalkanoates by micro-organisms

Liu

Zhang

et al. 2015

Lett Appl Microbiol

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Polyhydroxyalkanoates (PHAs) are polyesters accumulated by many bacteria under unbalanced growth conditions, and have been used to meet the various demands in areas of agriculture, medicine, and materials especially belong to a rapidly expanding area of biomedical research. Unfortunately, the high production cost than the traditional synthetic materials has greatly limited the wide application of PHA. Here, we systematically summarized recent progress in production of PHAs and a series of optimization strategies such as supplying renewable carbon substrates, developing better bacterial strains, optimization of fermentation processes, engineering new pathways and etc., were applied to reduce production cost, therefore providing many new ideas and methods for the production of PHAs in economically viable processes. This is believed to be a comprehensive report to show different strategies and methods for low-cost production of PHAs. Further studies are still needed to make PHAs more and more economically viable to meet a wide range of applicability.

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Thermophilic production of polyhydroxyalkanoates by a novelAneurinibacillusstrain isolated from Gudao oilfield, China

Cited by 34 publications

References 38 publications

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Non‐sterilized fermentative production of acetoin with 2,3‐butanediol as a main byproduct from maize hydrolysate by a newly isolated thermophilic Bacillus strain

Recent strategies for efficient production of polyhydroxyalkanoates by micro-organisms

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