Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Koller, Martin

doi:10.15406/mojps.2017.01.00011

Cited by 37 publications

(39 citation statements)

References 104 publications

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“…This is the case of the marine strains or strains that need the addition of vitamins or complex carbon sources in the culture medium, such as Azohydromonas lata DSM 1123, Novosphingobium aromaticivorans DSM 12444 or Tolumonas auensis DSM 9187. In general, the more complex a culture medium is and the more extreme the growth conditions of a microorganism are, the greater the costs for scaling-up the process (Koller, 2017). Finally, five strains were selected for further testing and quantification of PHA production: Amycolatopsis mediterranei DSM 43304, Caulobacter segnis DSM Table 3.…”

Section: Resultsmentioning

confidence: 99%

In silico prospection of microorganisms to produce polyhydroxyalkanoate from whey: Caulobacter segnis DSM 29236 as a suitable industrial strain

Bustamante

Segarra

Tortajada

et al. 2019

Microbial Biotechnology

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Summary Polyhydroxyalkanoates ( PHA s) are polyesters of microbial origin that can be synthesized by prokaryotes from noble sugars or lipids and from complex renewable substrates. They are an attractive alternative to conventional plastics because they are biodegradable and can be produced from renewable resources, such as the surplus of whey from dairy companies. After an in silico screening to search for ß‐galactosidase and PHA polymerase genes, several bacteria were identified as potential PHA producers from whey based on their ability to hydrolyse lactose. Among them, Caulobacter segnis DSM 29236 was selected as a suitable strain to develop a process for whey surplus valorization. This microorganism accumulated 31.5% of cell dry weight ( CDW ) of poly(3‐hydroxybutyrate) ( PHB ) with a titre of 1.5 g l −1 in batch assays. Moreover, the strain accumulated 37% of CDW of PHB and 9.3 g l −1 in fed‐batch mode of operation. This study reveals this species as a PHA producer and experimentally validates the in silico bioprospecting strategy for selecting microorganisms for waste re‐valorization.

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Section: Resultsmentioning

confidence: 99%

In silico prospection of microorganisms to produce polyhydroxyalkanoate from whey: Caulobacter segnis DSM 29236 as a suitable industrial strain

Bustamante

Segarra

Tortajada

et al. 2019

Microbial Biotechnology

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“…Especially exploration of extremophilic microbial strains settling in challenging environments, such as halophile microbes isolated from salt lakes, saltworks, or marine waters, is strongly increasing these days. Application of such production strains enables designing simple, flexible and robust cultivation processes, which can be energy-saving due to low or even without any sterility requirements (32)(33)(34)(35).…”

Section: Polyhydroxyalkanoates (Pha) -A Biological Alternativementioning

confidence: 99%

Advanced approaches to produce polyhydroxyalkanoate (PHA) biopolyesters in a sustainable and economic fashion

Koller

Braunegg

2018

The EuroBiotech Journal

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Polyhydroxyalkanoates (PHA), the only group of "bioplastics" sensu stricto, are accumulated by various prokaryotes as intracellular "carbonosomes". When exposed to exogenous stress or starvation, presence of these microbial polyoxoesters of hydroxyalkanoates assists microbes to survive."Bioplastics" such as PHA must be competitive with petrochemically manufactured plastics both in terms of material quality and manufacturing economics. Cost-effectiveness calculations clearly show that PHA production costs, in addition to bioreactor equipment and downstream technology, are mainly due to raw material costs. The reason for this is PHA production on an industrial scale currently relying on expensive, nutritionally relevant "1 st -generation feedstocks", such as like glucose, starch or edible oils. As a way out, carbon-rich industrial waste streams ("2 nd -generation feedstocks") can be used that are not in competition with the supply of food; this strategy not only reduces PHA production costs, but can also make a significant contribution to safeguarding food supplies in various disadvantaged parts of the world. This approach increases the economics of PHA production, improves the sustainability of the entire lifecycle of these materials, and makes them unassailable from an ethical perspective.In this context, our EU-funded projects ANIMPOL and WHEYPOL, carried out by collaborative consortia of academic and industrial partners, successfully developed PHA production processes, which resort to waste streams amply available in Europe. As real 2 nd -generation feedstocks", waste lipids and crude glycerol from animal-processing and biodiesel industry, and surplus whey from dairy and cheese making industry were used in these processes. Cost estimations made by our project partners determine PHA production prices below 3 € (WHEYPOL) and even less than 2 € (ANIMPOL), respectively, per kg; these values already reach the benchmark of economic feasibility.The presented studies clearly show that the use of selected high-carbon waste streams of (agro)industrial origin contributes significantly to the cost-effectiveness and sustainability of PHA biopolyester production on an industrial scale.

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“…[17b] Recombinant H. campaniensis overexpressing PHB synthesis genes phb CAB achieved stably 70 wt% PHB accumulation in CDW utilizing the mixed substrates for 65 d. Thus, H. campaniensis LS21 definitely will be a cost and energy‐efficient platform strain for further engineering. [17b,48]…”

Section: Halophilic Chassis For Bioproductionmentioning

confidence: 99%

Halophiles as Chassis for Bioproduction

2018

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Industrial biotechnology aims to solve the challenges of petroleum‐based production using microorganisms as catalysts. However, current industrial biotechnology suffers from high energy and fresh water consumption as well as difficult downstream purification. In addition, most industrial microorganisms are not resistant to other microbial contaminations, and this seriously compromises process effectiveness and the economy. The recently proposed “next‐generation industrial biotechnology” employs contamination resistant microorganisms, including alkaliphilic, acidophilic, thermophilic or halophilic bacteria, etc., for bioproduction. The most successful example is the use of halophilic bacteria as chassis for the production of chemicals, proteins, and biopolymers. This review summarizes some of the most recent studies to engineer the halophilic chassis Halomonas spp. for the production of chemicals, proteins, and several biopolyesters such as poly(3‐hydroxybutyrate), copolymers of 3‐hydroxybutyrate and 3‐hydroxyvalerate, and copolymers of 3‐hydroxybutyrate and 4‐hydroxybutyrate. The Halomonas chassis is also successfully engineered to change its morphology from short bars to long fibers and large spheres for convenient gravity separation. This simplifies the bioprocessing.

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Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Cited by 37 publications

References 104 publications

In silico prospection of microorganisms to produce polyhydroxyalkanoate from whey: Caulobacter segnis DSM 29236 as a suitable industrial strain

In silico prospection of microorganisms to produce polyhydroxyalkanoate from whey: Caulobacter segnis DSM 29236 as a suitable industrial strain

Advanced approaches to produce polyhydroxyalkanoate (PHA) biopolyesters in a sustainable and economic fashion

Halophiles as Chassis for Bioproduction

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