Thermophilic biohydrogen production from palm oil mill effluent: Effect of immobilized cells on granular activated carbon in fluidized bed reactor

Jamali, Nur Syakina; Rashidi, Nur Farahana Dzul; Jahim, Jamaliah Md; O‐Thong, Sompong; Jehlee, Aminee; Engliman, Nurul Sakinah

doi:10.1016/j.fbp.2019.07.012

Cited by 29 publications

(13 citation statements)

References 23 publications

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“…The rod-shaped bacterial cells appeared to be the dominant consortium on the GAC based on their morphologic properties. This study is in agreement with a previous study by Jamali et al [21]. It has been identified that the dominant species of anaerobic hydrogen producers is Thermoanaerobacterium thermosaccharolyticum, which is stated to have spores and is rod-shaped and Gram-positive [21,28].…”

Section: Sem Of Immobilized Beadssupporting

confidence: 93%

“…This indicates that 20 g/L is the optimal amount of sugar to be used for immobilisation beads and, thus, as the optimum substrate for future experiments. In parallel, our previous work also suggested that 20 g/L sugar was the optimal amount of sugar to use [21]. From the data obtained, it was found that the granular activated carbon could provide a The process continued until the biogas was stable and ready for gaseous analysis by using GC.…”

Section: Microbial Cells Self-attached To Gac For Hydrogen Productionmentioning

confidence: 81%

“…Hence, the study revealed that a combination of GAC and alginate as immobilised beads gave a positive response in bacterial immobilisation, especially in promoting the growth of hydrogen-producing bacteria during the fermentation [34]. The positive performance of the GAC-Alg beads was due to the presence of granule activated carbon inside, which acted as a support for the alginate carrier and maintained the stability of beads [21].…”

Section: Biohydrogen Production Of Immobilised Beads Gac-algmentioning

confidence: 93%

“…xylose 10 g L −1 , and glucose 10 g L −1 . The fermentation system was cultivated for 48 h in a water bath shaker at 60 • C and 120 rpm, with the pH of the culture medium adjusted to pH 6.0 [21,22].…”

Section: Biofilm Formation On Activated Carbonmentioning

confidence: 99%

“…GAC has a high surface area, low toxicity, and excellent mechanical properties, which are ideal for fermentation at high temperatures. Moreover, its characteristic of having highly porous structure helps to preserve cell viability, which serves an excellent purpose in the field of microbial colonisation where fermentative bacteria can expand freely on the surface of the supporting material and form a biofilm [21]. Efforts have been made to improve the high cell density that facilitates the good production of hydrogen.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Alginate and Chitosan on Activated Carbon as Immobilisation Beads in Biohydrogen Production

et al. 2020

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In this study, the effects of alginate and chitosan as entrapped materials in the biofilm formation of microbial attachment on activated carbon was determined for biohydrogen production. Five different batch fermentations, consisting of mixed concentration alginate (Alg), were carried out in a bioreactor at temperature of 60 °C and pH 6.0, using granular activated carbon (GAC) as a primer for cell attachment and colonisation. It was found that the highest hydrogen production rate (HPR) of the GAC–Alg beads was 2.47 ± 0.47 mmol H2/l.h, and the H2 yield of 2.09 ± 0.22 mol H2/mol sugar was obtained at the ratio of 2 g/L of Alg concentration. Next, the effect of chitosan (C) as an external polymer layer of the GAC–Alg beads was investigated as an alternative approach to protecting the microbial population in the biofilm in a robust environment. The formation of GAC with Alg and chitosan (GAC–AlgC) beads gave the highest HPR of 0.93 ± 0.05 mmol H2/l.h, and H2 yield of 1.11 ± 0.35 mol H2/mol sugar was found at 2 g/L of C concentration. Hydrogen production using GAC-attached biofilm seems promising to achieve consistent HPRs at higher temperatures, using Alg as immobilised bead material, which has indicated a positive response in promoting the growth of hydrogen-producing bacteria and providing excellent conditions for microorganisms to grow and colonise high bacterial loads in a bioreactor.

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Section: Sem Of Immobilized Beadssupporting

confidence: 93%

Section: Microbial Cells Self-attached To Gac For Hydrogen Productionmentioning

confidence: 81%