Thermodynamic and steady-state-kinetic investigation of the effect of NN′-dicyclohexylcarbodi-imide on H+ translocation by the mitochondrial cytochrome bc1 complex
Abstract:Steady-state kinetic measurements showed that NN'-dicyclohexylcarbodi-imide decreased the observed H+/2e ratio of H+ transport by mitochondria respiring on succinate, acting mainly at the cytochrome bc1 complex. Thermodynamic assessment of the H+/2e ratio by measuring the force ratio across the bc1 complex showed that the inhibitor did not affect H+ translocation. Possible explanations of this disagreement between methods are examined; we conclude that the inhibitor does not alter the mechanistic stoichiometry… Show more
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