The respiratory-chain NADH dehydrogenase (complex I) of mitochondria

Weiss, Hanns; Friedrich, Thorsten; Hofhaus, Götz; Preis, Dagmar

doi:10.1007/978-3-642-77200-9_5

Cited by 121 publications

(152 citation statements)

References 151 publications

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“…Therefore, these polymorphisms may influence the functions of the complex, such as electron transport, electron leakage and reactive oxygen species production. 21 A number of studies failed to identify associations between mtDNA and sperm motility. The discordance among haplotype studies is most likely a reflection of the difficulty in matching cases and controls and the existence of genuine variations in the genome of different populations.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial DNA haplogroup associated with sperm motility in the Han population

Feng

Zhang²,

Lin³

et al. 2013

Asian J Androl

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In this study, we aimed to determine whether the main mitochondrial DNA (mtDNA) haplogroups of the Han people have an impact on spermatozoa motility. We recruited 312 men who were consecutively admitted to two affiliated hospitals of College of Medicine, Zhejiang University from May 2011 to April 2012 as part of fertility investigations. Semen and whole blood samples were collected from the men. We determined the mtDNA haplogroups by analysing the sequences of mtDNA hypervariable segment I and testing diagnostic polymorphisms in the mtDNA coding region with DNA probes. No significant differences were found in the clinical characteristics of the mtDNA haplogroup R and non-R (P .0.05). Our results suggest that mtDNA haplogroup R is a strong independent predictor of sperm motility in the Han population, conferring a 2.97-fold (95% confidence interval: 1.74-4.48, P,0.001) decreased chance of asthenozoospermia compared with those without haplogroup R.

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Section: Discussionmentioning

confidence: 99%

Mitochondrial DNA haplogroup associated with sperm motility in the Han population

Feng

Zhang²,

Lin³

et al. 2013

Asian J Androl

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“…IGFBP-3, as well as several other IGFBPs, can also promote proliferation by a regulated release of insulin-like growth factor, which protects its receptor from down-regulation by exposure to high concentrations of insulin-like growth factor (Conover and Powell, 1991). One of the recently identified genes, which was selectively expressed in RCC, is a homologue of a human MLRQ subunit of the NADH: ubiquinone oxidoreductase, known as the first and largest enzyme of the mitochondrial respiratory chain (Weiss et al, 1991). Though additional experiments are required for defining the function of this subunit, we hypothesize that this molecule is responding to the high metabolic demand of tumour cells.…”

Section: Discussionmentioning

confidence: 99%

Identification of human renal cell carcinoma associated genes by suppression subtractive hybridization

et al. 2001

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Renal cell carcinoma (RCC) accounts for approximately 3% of adult malignancies and 1.4% of cancer-related deaths (Reis and Faria, 1994). The prognosis of RCC remains poor. One third of the patients already have metastases when first consulting the hospital. Another 30-40% of patients develop metastases after surgical excision of the primary tumour (Ravaud and Debled, 1999). RCC are radioresistant (Nieder et al, 1996) and more than 80% are chemoresistant (Mickisch, 1994). Since RCC are presumed to be immunogenic, several clinical trials are exploring the efficacy of cytokines, mainly interleukin 2 (IL2) and/or interferon-α (IFNα), and the transfer of lymphokine-activated killer cells (Hofmockel et al, 1997;Bukowski, 2000;Hoffman et al, 2000). Despite these new options, the median survival time of patients with metastatic disease still remains only 6-8 months and the overall 5-year survival rate is less than 5% (Moch et al, 2000;Motzer and Russo, 2000). Thus, there is an urgent requirement for alternative therapeutic modalities.The current strategy is to design therapeutic approaches based on specific biological features of each tumour type. These include (i) the aberrant expression of genes which can be recognized by the immune system as foreign (Pawelec et al, 1999;Wang and Rosenberg, 1999;Bremers and Parmiani, 2000); (ii) gene products related to the formation of new blood vessels (neoangiogenesis), since they are essential for tumour expansion and metastatic settlement (Harris and Thorgeirsson, 1998;Kerbel, 2000;Rosen, 2000) and (iii) the altered expression of adhesion molecules, matrix-degrading enzymes, their receptors and inhibitors, which are a further requisite of metastatic spread (Huang et al, 1997;Yu et al, 1997).Several technique enable the identification of tumour markers. Subtractive hybridization (Lamar and Palmer, 1984; Kunkel et al, 1985;Kuang et al, 1998), differential display reverse transcription-polymerase chain reaction (DD RT-PCR; Liang and Pardee, 1992) and hybridization of cDNA microarrays (reviewed in Khan et al, 1999) are frequently used to compare the expression patterns between tumour and normal tissue. Other approaches, such as serological screening (SEREX;Sahin et al, 1995) and screening of cytotoxic T lymphocyte activity against an autologous tumour cell line (De Plaen et al, 1988), are especially focused on the identification of immunogenic tumour molecules.We have described recently the successful use of SSH using matched RCC and normal kidney tissue . In this study, we randomly selected 16 genes, which by SSH appeared to be differentially expressed. Differential expression of 9 of these 16 genes could be verified by Northern blot analysis. 2 of the 9 genes appeared to be novel. From the remaining 7 genes, expression of 5 had been associated with the malignant phenotype. To substantiate that SSH is a suitable method for the identification of differentially expressed genes, we performed a SSH with an additional pair of normal renal and RCC tissue and compared the validity of SSH ...

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“…This protein is crucial for cellular respiration and is expressed ubiquitously (56). How is it possible that a self-peptide (ND1) that acts as a weak agonist does not activate peripheral T cells?…”

Section: Discussionmentioning

confidence: 99%

A Physiological Ligand of Positive Selection Is Recognized as a Weak Agonist

Berg

Irion

Kattman

et al. 2000

The Journal of Immunology

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Positive selection is a process that ensures that peripheral T cells express TCR that are self-MHC restricted. This process occurs in the thymus and requires both self-MHC and self-peptides. We have recently established a TCR transgenic (TCRtrans+) mouse model using the C10.4 TCR restricted to the MHC class Ib molecule, H2-M3. Having defined H2-M3 as the positively selecting MHC molecule, the severely limited number of H2-M3 binding peptides allowed us to characterize a mitochondrial NADH dehydrogenase subunit 1-derived 9-mer peptide as the physiological ligand of positive selection. Here, we demonstrate that the NADH dehydrogenase subunit 1 self-peptide is seen by mature C10.4 TCRtrans+ T cells as a weak agonist and induces positive selection at a defined concentration range. We also found that the full-length cognate peptide, a strong agonist for mature C10.4 TCRtrans+ T cells, initiated positive selection, albeit at significantly lower concentrations. At increased peptide concentrations, and thus increased epitope densities, either peptide only induced the development of partially functional T cells. We conclude that successful positive selection only proceeded at a defined, yet fairly narrow window of avidity.

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The respiratory-chain NADH dehydrogenase (complex I) of mitochondria

Cited by 121 publications

References 151 publications

Mitochondrial DNA haplogroup associated with sperm motility in the Han population

Mitochondrial DNA haplogroup associated with sperm motility in the Han population

Identification of human renal cell carcinoma associated genes by suppression subtractive hybridization

A Physiological Ligand of Positive Selection Is Recognized as a Weak Agonist

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