Thermoanaerobacter siderophilus sp. nov., a novel dissimilatory Fe(III)-reducing, anaerobic, thermophilic bacterium

Slobodkin, A. I.; Tourova, T. P.; Кузнецов, Б. Б.; Kostrikina, N. A.; Chernyh, N. A.; Bonch-Osmolovskaya, E. A.

doi:10.1099/00207713-49-4-1471

Cited by 125 publications

(111 citation statements)

References 29 publications

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“…Light-brown, irregular-shaped colonies, 0.5-1.0 mm in diameter, appeared after 3 days of incubation at 60 u C. Individual colonies were checked for manganese-reducing ability by transferring them to liquid medium with MnO 2 and, for positive cultures, the process of obtaining colonies was repeated two more times. Finally, a single colony that had developed in the highest agar block dilution (10 26 ) and possessed Mn(IV)-reducing ability was designated strain SLM 61 T and used for further studies.Physiological studies on substrate and electron acceptor utilization, temperature, pH and salinity ranges for growth, light and electron microscopy, analytical techniques, DNA extraction and determination of G+C content were performed as described previously (Slobodkin et al, 1999). Manganese (Mn 2+ ) contents were determined by the formaldoxime method (Goto et al, 1962).…”

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“…Physiological studies on substrate and electron acceptor utilization, temperature, pH and salinity ranges for growth, light and electron microscopy, analytical techniques, DNA extraction and determination of G+C content were performed as described previously (Slobodkin et al, 1999). Manganese (Mn 2+ ) contents were determined by the formaldoxime method (Goto et al, 1962).…”

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“…Enrichment cultures were initiated by inoculation of 10 % (w/v) of the sample into anaerobically prepared, bicarbonate-buffered, sterile (135 u C, 1 h) liquid medium with lactate (14 mM) as an electron donor and manganese(IV) oxide (25 mmol l 21 ) as an electron acceptor. Medium composition and preparation techniques were described previously (Slobodkin et al, 1999). MnO 2 (birnessite) was prepared by O 2 (air) oxidation of MnCl 2 in alkali medium (Feng et al, 2004).…”

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Carboxydocella manganica sp. nov., a thermophilic, dissimilatory Mn(IV)- and Fe(III)-reducing bacterium from a Kamchatka hot spring

Slobodkina

Panteleeva

Sokolova

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

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A thermophilic, anaerobic, dissimilatory Mn(IV)-and Fe(III)-reducing bacterium (strain SLM 61 T ) was isolated from a terrestrial hot spring on the Kamchatka peninsula. The cells were straight rods, 0.5-0.6 mm in diameter and 1.0-6.0 mm long, and exhibited tumbling motility by means of peritrichous flagellation. The strain grew at 26-70 6C, with an optimum at 58-60 6C, and at pH 5.5-8.0, with an optimum at pH 6.5. Growth of SLM 61 T was observed at 0-2.0 % (w/v) NaCl, with an optimum at 0.5 % (w/v). The generation time under optimal growth conditions was 40 min. Strain SLM 61 T grew and reduced Mn(IV), Fe(III) or nitrate with a number of organic acids and complex proteinaceous compounds as electron donors. It was capable of chemolithoautotrophic growth using molecular hydrogen as an electron donor, Fe(III) but not Mn(IV) or nitrate as an electron acceptor and CO 2 as a carbon source. It also was able to ferment pyruvate, yeast extract, glucose, fructose, sucrose and maltose. The G+C content of DNA of strain SLM 61 T was 50.9 mol%. 16S rRNA gene sequence analysis revealed that the closest relative of the isolated organism was Carboxydocella thermautotrophica 41 T (96.9 % similarity). On the basis of its physiological properties and phylogenetic analyses, the isolate is considered to represent a novel species, for which the name Carboxydocella manganica sp. nov. is proposed. The type strain is SLM 61 T (5DSM 23132 T 5VKM B-2609 T ). C. manganica is the first described representative of the genus Carboxydocella that possesses the ability to reduce metals and does not utilize CO.Metal-reducing micro-organisms play important roles in the cycling of carbon and metals in various anaerobic ecosystems, including thermal environments. Dissimilatory Fe(III)-and Mn(IV)-reducing micro-organisms have been found in a variety of thermal biotopes, including continental hot springs, a deep terrestrial subsurface, submarine petroleum reservoirs, deep-sea hydrothermal vents and anthropogenic systems (Lovley et al., 2004;Slobodkin, 2005). Dissimilatory manganese reduction by thermophilic prokaryotes is much less well studied than iron reduction. Of 43 described species of Fe(III)-reducing thermophiles belonging to different phylogenetic groups of the Bacteria and Archaea, the capacity for Mn(IV) reduction has been reported for six species (Slobodkin, 2005). Only two micro-organisms, Bacillus infernus (Boone et al., 1995) and Deferribacter thermophilus (Greene et al., 1997), were first isolated using Mn(IV) as an electron acceptor. In this article, we report the isolation and characterization of a novel anaerobic, thermophilic, Mn(IV)-and Fe(III)-reducing micro-organism from a hydrothermal spring on the Kamchatka peninsula.Strain SLM 61 T was isolated from a sample of sediment collected from a hot spring at the 'Trostnikovyi' field, Uzon Caldera, Kamchatka, Russia. The conditions at the sampling site were 60 u C, pH 6.2, E h 2400 mV. Samples were taken anaerobically in tightly stoppered bottles and transported to the laboratory. Enri...

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Carboxydocella manganica sp. nov., a thermophilic, dissimilatory Mn(IV)- and Fe(III)-reducing bacterium from a Kamchatka hot spring

Slobodkina

Panteleeva

Sokolova

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

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“…An enrichment culture was initiated by inoculation of 10 % (w/v) of the sample into anaerobically prepared, bicarbonate-buffered, sterile (135 u C, 1 h) liquid medium with acetate (18 mM) as an electron donor and poorly crystalline Fe(III) oxide (90 mmol l 21 ) as an electron acceptor. Medium composition and preparation techniques were as described previously (Slobodkin et al, 1999a); the medium was additionally supplemented with NaCl (18 g l 21 ) and MgCl 2 (4 g l 21 ) to increase the salinity. After three subsequent transfers and following serial 10-fold dilutions in the same medium at 65 u C, two morphological cell-types were observed in the highest positive dilution (10 25 ): rods and cocci.…”

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Geoglobus acetivorans sp. nov., an iron(III)-reducing archaeon from a deep-sea hydrothermal vent

Slobodkina

Kolganova

Quérellou

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A hyperthermophilic, anaerobic, dissimilatory Fe(III)-reducing, facultatively chemolithoautotrophic archaeon (strain SBH6 T ) was isolated from a hydrothermal sample collected from the deepest of the known World Ocean hydrothermal fields, Ashadze field (126 589 210 N 446 519 470 W) on the Mid-Atlantic Ridge, at a depth of 4100 m. The strain was enriched using acetate as the electron donor and Fe(III) oxide as the electron acceptor. Cells of strain SBH6 T were irregular cocci, 0.3-0.5 mm in diameter. The temperature range for growth was 50-85 6C, with an optimum at 81 6C. The pH range for growth was 5.0-7.5, with an optimum at pH 6.8. Growth of SBH6 T was observed at NaCl concentrations ranging from 1 to 6 % (w/v) with an optimum at 2.5 % (w/v). The isolate utilized acetate, formate, pyruvate, fumarate, malate, propionate, butyrate, succinate, glycerol, stearate, palmitate, peptone and yeast extract as electron donors for Fe(III) reduction. It was also capable of growth with H 2 as the sole electron donor, CO 2 as a carbon source and Fe(III) as an electron acceptor without the need for organic substances. Fe(III) [in the form of poorly crystalline Fe(III) oxide or Fe(III) citrate] was the only electron acceptor that supported growth. 16S rRNA gene sequence analysis revealed that the closest relative of the isolated organism was Geoglobus ahangari 234 T (97.0 %). On the basis of its physiological properties and phylogenetic analyses, the isolate is considered to represent a novel species, for which the name Geoglobus acetivorans sp. nov. is proposed. The type strain is SBH6 T (5DSM 21716 T 5VKM B-2522 T ).Iron minerals are abundant in deep-sea hydrothermal vents. The surfaces of active chimneys are frequently covered with deposits of iron oxides in different oxidative states, and the amount of iron in hydrothermal fluid can reach molar concentrations. Thus, deep-sea hydrothermal vents can provide an ecological niche for Fe(III)-reducing micro-organisms (Slobodkin et al., 2001). However, only a few thermophilic Fe(III)-reducers have been isolated from this environment. Currently, thermophilic and hyperthermophilic iron-reducing micro-organisms recovered from deep-sea habitats include two species of the Bacteria, Geothermobacter ehrlichii (Kashefi et al., 2003) and Deferribacter abyssi (Miroshnichenko et al., 2003), and three representatives of the Archaea, Thermococcus sp. SN531 (Slobodkin et al., 2001), Geoglobus ahangari (Kashefi et al., 2002) and 'Candidatus Aciduliprofundum boonei ' (Reysenbach et al., 2006). In this paper, we report the isolation and characterization of a novel hyperthermophilic Fe(III)-reducing archaeon from the deepest of the known World Ocean hydrothermal fields.Strain SBH6 T was isolated from a fragment of a hydrothermal chimney-like structure. The sample was collected in March 2007 during the Serpentine cruise at the Ashadze hydrothermal field (12 u 589 210 N 44 u 519 470 W) on the Mid-Atlantic Ridge at a depth of 4100 m. For sample collection, sterilized microbiological boxes filled with...

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“…The genus Tepidibacter was proposed by Slobodkin et al (2003) with Tepidibacter thalassicus as the type species. Tepidibacter formicigenes was subsequently described by Urios et al (2004).…”

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Tepidibacter mesophilus sp. nov., a mesophilic fermentative anaerobe isolated from soil polluted by crude oil, and emended description of the genus Tepidibacter

Tan

Zhang

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

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A mesophilic, aerotolerant, endospore-forming, fermentative bacterium, designated strain B1T, was isolated from soil polluted by crude oil in the Karamay Oil Field, China. Cells were Gram-positive, rod-shaped, 1.1–1.6 µm wide and 2.3–4.7 µm long, and were motile by means of peritrichous flagella. Growth occurred at 10–40 °C and pH 6.0–8.9; optimal growth occurred at 28–32 °C and pH 7.3. The optimal concentrations of NaCl and sea salts for growth were 0.5 and 1 % (w/v), respectively. The strain was halotolerant and grew in the presence of NaCl or sea salts up to a concentration of 9 % (w/v). Substrates utilized as sole carbon sources were beef extract, yeast extract, peptone, tryptone, casein, d-glucose, d-fructose, d-xylose, d-ribose, d-galactose, maltose, l-rhamnose, trehalose, l-valine, dl-alanine plus l-proline and dl-alanine plus l-glycine. The main products of glucose fermentation were ethanol and acetate. iso-C15 : 0, iso-C14 : 0, C16 : 0 and iso-C13 : 0 were the major fatty acids. 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus Tepidibacter, showing 94.7 and 94.1 % similarity to the type strains of Tepidibacter formicigenes and Tepidibacter thalassicus, respectively. The genomic DNA G+C content of strain B1T was 29.8 mol%. On the basis of its phenotypic and genotypic properties, strain B1T is suggested to represent a novel species of the genus Tepidibacter, for which the name Tepidibacter mesophilus sp. nov. is proposed. The type strain is B1T ( = CGMCC 1.5148T = JCM 16806T).

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Thermoanaerobacter siderophilus sp. nov., a novel dissimilatory Fe(III)-reducing, anaerobic, thermophilic bacterium

Cited by 125 publications

References 29 publications

Carboxydocella manganica sp. nov., a thermophilic, dissimilatory Mn(IV)- and Fe(III)-reducing bacterium from a Kamchatka hot spring

Carboxydocella manganica sp. nov., a thermophilic, dissimilatory Mn(IV)- and Fe(III)-reducing bacterium from a Kamchatka hot spring

Geoglobus acetivorans sp. nov., an iron(III)-reducing archaeon from a deep-sea hydrothermal vent

Tepidibacter mesophilus sp. nov., a mesophilic fermentative anaerobe isolated from soil polluted by crude oil, and emended description of the genus Tepidibacter

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