Tepidibacter mesophilus sp. nov., a mesophilic fermentative anaerobe isolated from soil polluted by crude oil, and emended description of the genus Tepidibacter

Abstract

A mesophilic, aerotolerant, endospore-forming, fermentative bacterium, designated strain B1T, was isolated from soil polluted by crude oil in the Karamay Oil Field, China. Cells were Gram-positive, rod-shaped, 1.1–1.6 µm wide and 2.3–4.7 µm long, and were motile by means of peritrichous flagella. Growth occurred at 10–40 °C and pH 6.0–8.9; optimal growth occurred at 28–32 °C and pH 7.3. The optimal concentrations of NaCl and sea salts for growth were 0.5 and 1 % (w/v), respectively. The strain was halotolerant and grew in the presence of NaCl or sea salts up to a concentration of 9 % (w/v). Substrates utilized as sole carbon sources were beef extract, yeast extract, peptone, tryptone, casein, d-glucose, d-fructose, d-xylose, d-ribose, d-galactose, maltose, l-rhamnose, trehalose, l-valine, dl-alanine plus l-proline and dl-alanine plus l-glycine. The main products of glucose fermentation were ethanol and acetate. iso-C15 : 0, iso-C14 : 0, C16 : 0 and iso-C13 : 0 were the major fatty acids. 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus Tepidibacter, showing 94.7 and 94.1 % similarity to the type strains of Tepidibacter formicigenes and Tepidibacter thalassicus, respectively. The genomic DNA G+C content of strain B1T was 29.8 mol%. On the basis of its phenotypic and genotypic properties, strain B1T is suggested to represent a novel species of the genus Tepidibacter, for which the name Tepidibacter mesophilus sp. nov. is proposed. The type strain is B1T ( = CGMCC 1.5148T = JCM 16806T).

Bacterial and archeal community composition in hot springs from Indo-Burma region, North-east India

“…The genus Tepidibacter ( Firmicutes ) was proposed (Slobodkin et al 2003) with three reported type species i.e. Tepidibacter thalassicus, Tepidibacter formicigenes and Tepidibacter mesophilus (Tan et al 2012). Thus, the OTUs from Jakrem hot spring representing Tepidibacter ( Firmicutes ) possibly are the novel species with in this genus.…”

Egypt's Red Sea coast: phylogenetic analysis of cultured microbial consortia in industrialized sites

“…Psychrilyobacter produces H2 and acetate and has the ability to degrade hexahydro-1,3,5-trinitro-1,3,5-triazine and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine, two nitramine explosives (Zhao et al, 2009). Tepidibacter was isolated from an oil field in China (Tan et al, 2012). Photobacteriumspecies show mercuric resistance (Reyes et al, 1999), are stable in biodiesel production at high methanol concentrations (Yang et al, 2009) and have high oil emulsification activity (Ryu et al, 2006).…”

Draft Genome Sequence of Tepidibacter mesophilus Strain JCM 16806 T Isolated from Soil Polluted by Crude Oil in China

“…The former two species are thermophilic and isolated from hydrothermal vents, but the latter one is mesophilic and isolated from soil contaminated by crude oil. T. mesophilus strain JCM 16806 T is anaerobic, aerotolerant, endospore forming, and fermentative; optimal growth occurred at 28 to 32°C ( 3 ).…”