Thermoacidophilic Microorganisms and their Novel Biocatalysts

Bertoldo, Costanzo; Dock, Christiane; Antranikian, Garabed

doi:10.1002/elsc.200402155

Cited by 42 publications

(12 citation statements)

References 57 publications

(47 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Belonging mostly to the Archaea domain, they exist in many phylogenetically different groups and their biotopes (submarine hydrothermal areas, solfatara) exist since the Archaean period (Stetter et al 1990). Moreover, thermoacidophilic microorganisms are reported as potential sources of rare and robust extracellular heat and acid-stable biocatalysts for biotechnological applications (Bertoldo et al 2004). Table 2 summarizes selected parameters of the growth of Sulfolobus species cultivated on different substrates.…”

Section: Introductionmentioning

confidence: 99%

Phenol biodegradation by the thermoacidophilic archaeon Sulfolobus solfataricus 98/2 in a fed-batch bioreactor

et al. 2010

View full text Add to dashboard Cite

Toxic at low concentrations, phenol is one of the most common organic pollutants in air and water. In this work, phenol biodegradation was studied in extreme conditions (80°C, pH = 3.2) in a 2.7 l bioreactor with the thermoacidophilic archaeon Sulfolobus solfataricus 98/2. The strain was first acclimatized to phenol on a mixture of glucose (2000 mg l(-1)) and phenol (94 mg l(-1)) at a constant dissolved oxygen concentration of 1.5 mg l(-1). After a short lag-phase, only glucose was consumed. Phenol degradation then began while glucose was still present in the reactor. When glucose was exhausted, phenol was used for respiration and then for biomass build-up. After several batch runs (phenol < 365 mg l(-1)), specific growth rate (μ(X)) was 0.034 ± 0.001 h(-1), specific phenol degradation rate (q(P)) was 57.5 ± 2 mg g(-1) h(-1), biomass yield (Y(X/P)) was 52.2 ± 1.1 g mol(-1), and oxygen yield factor (Y(X/O2)) was 9.2 ± 0.2 g mol(-1). A carbon recovery close to 100% suggested that phenol was exclusively transformed into biomass (35%) and CO(2) (65%). Molar phenol oxidation constant (Y(O2/P)) was calculated from stoichiometry of phenol oxidation and introducing experimental biomass and CO(2) conversion yields on phenol, leading to values varying between 4.78 and 5.22 mol mol(-1). Respiratory quotient was about 0.84 mol mol(-1), very close to theoretical value (0.87 mol mol(-1)). Carbon dioxide production, oxygen demand and redox potential, monitored on-line, were good indicators of growth, substrate consumption and exhaustion, and can therefore be usefully employed for industrial phenol bioremediation in extreme environments.

show abstract

Section: Introductionmentioning

confidence: 99%

Phenol biodegradation by the thermoacidophilic archaeon Sulfolobus solfataricus 98/2 in a fed-batch bioreactor

et al. 2010

View full text Add to dashboard Cite

show abstract

“…The presence of euryarchaeotes can be explained by the fact that in moderately thermal (40-60°C) acidic environments, the phyla Euryarchaeota, Crenarchaeota and some gram-positive bacteria may coexist. Within Euryarchaeota, phylum Thermoplasma has been reported to be moderately thermophilic (45-67°C) and acidophilic (pH 0.5-4.0) (Bertoldo et al 2004). Picrophilus torridus, with a temperature range of 45-65°C, has the lowest recorded pH at or below zero (Schleper et al 1995;Futterer et al 2004) with optimal pH for growth at 0.7.…”

Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

Microbial community of a volcanic mudspring in the Philippines as revealed by 16S rDNA sequence analysis and fluorescence in situ hybridization

Lantican

Diaz

Cantera

et al. 2010

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Mt. Makiling Mudspring in Laguna, Philippines is a thermophilic, acidophilic environment that previously has been shown to harbor novel microorganisms. We assessed the microbial community that exists at this volcanic mudspring using 16S rRNA-based approaches. DNA was extracted from solfataric soils and sediments taken from Mudspring. The 16S rDNA was PCR amplified using universal (519F-1392R) and archaeal-specific (23FPL-1391R) primer pairs, cloned, and sequenced. Phylogenetic analysis of the cloned 16S rDNA showed that eleven clones clustered with, and therefore related to Sulfolobus tokodaii 7 and two clones clustered with S. solfataricu, S. shibatae and S. islandicus. Three clone sequences were related to those found in thermophilic chalcopyrite (CuFeS 2 ), a copper sulfuric ore from bioleaching reactors. One clone had low similarity (95% identity) with uncultured archaeon clone KOZ184. Fluorescence in situ hybridization (FISH) analysis revealed that about 71% of the microbial community present in the Mudspring belong to domain Archaea of which 63% were Crenarchaeota and 8% were Euryarchaeota. Seventeen percent (17%) of the population consisted of bacteria as indicated by the positive hybridization with the BACT338 probe, and the remaining 12% are unidentified. This study is the first attempt to use molecular techniques in any environment in the Philippines.

show abstract

“…Most of the thermophilic microorganisms belong to the Archaea group, grow at low pH and usually live in extreme environments such as solfataric fields or submarine hydrothermal areas [23] . Their physiological characteristics and the general features of their genome sequences have been reviewed elsewhere [24] .…”

Section: Introductionmentioning

confidence: 99%

Biochemical, Transcriptional and Translational Evidences of the Phenol-meta-Degradation Pathway by the Hyperthermophilic Sulfolobus solfataricus 98/2

et al. 2013

View full text Add to dashboard Cite

Phenol is a widespread pollutant and a model molecule to study the biodegradation of monoaromatic compounds. After a first oxidation step leading to catechol in mesophilic and thermophilic microorganisms, two main routes have been identified depending on the cleavage of the aromatic ring: ortho involving a catechol 1,2 dioxygenase (C12D) and meta involving a catechol 2,3 dioxygenase (C23D). Our work aimed at elucidating the phenol-degradation pathway in the hyperthermophilic archaea Sulfolobus solfataricus 98/2. For this purpose, the strain was cultivated in a fermentor under different substrate and oxygenation conditions. Indeed, reducing dissolved-oxygen concentration allowed slowing down phenol catabolism (specific growth and phenol-consumption rates dropped 55% and 39%, respectively) and thus, evidencing intermediate accumulations in the broth. HPLC/Diode Array Detector and LC-MS analyses on culture samples at low dissolved-oxygen concentration (DOC = 0.06 mg.L−1) suggested, apart for catechol, the presence of 2-hydroxymuconic acid, 4-oxalocrotonate and 4-hydroxy-2-oxovalerate, three intermediates of the meta route. RT-PCR analysis on oxygenase-coding genes of S. solfataricus 98/2 showed that the gene coding for the C23D was expressed only on phenol. In 2D-DIGE/MALDI-TOF analysis, the C23D was found and identified only on phenol. This set of results allowed us concluding that S. solfataricus 98/2 degrade phenol through the meta route.

show abstract

Thermoacidophilic Microorganisms and their Novel Biocatalysts

Cited by 42 publications

References 57 publications

Phenol biodegradation by the thermoacidophilic archaeon Sulfolobus solfataricus 98/2 in a fed-batch bioreactor

Phenol biodegradation by the thermoacidophilic archaeon Sulfolobus solfataricus 98/2 in a fed-batch bioreactor

Microbial community of a volcanic mudspring in the Philippines as revealed by 16S rDNA sequence analysis and fluorescence in situ hybridization

Biochemical, Transcriptional and Translational Evidences of the Phenol-meta-Degradation Pathway by the Hyperthermophilic Sulfolobus solfataricus 98/2

Contact Info

Product

Resources

About