“…Non-conductive samples such as biological specimen have to be covered with a fine layer of conductive material such as metals (palladium, iridium, gold) or carbon before they can be analyzed in the scanning electron microscope (SEM) under high vacuum conditions. If left uncoated electrons accumulate at the surface of the sample and lead to artifacts which are commonly referred to as charging [1][2][3][4]. Excessive charging leads to shifted line scans and distorted ultrastructure.…”
Section: Beam Deceleration Leads To Superior Image Quality Of Biologimentioning
“…Non-conductive samples such as biological specimen have to be covered with a fine layer of conductive material such as metals (palladium, iridium, gold) or carbon before they can be analyzed in the scanning electron microscope (SEM) under high vacuum conditions. If left uncoated electrons accumulate at the surface of the sample and lead to artifacts which are commonly referred to as charging [1][2][3][4]. Excessive charging leads to shifted line scans and distorted ultrastructure.…”
Section: Beam Deceleration Leads To Superior Image Quality Of Biologimentioning
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