Abstract:In this communication, molecularly imprinted nanoparticles (nanoMIPs) that are produced by solid-phase synthesis are functionalised onto thermistors via dip-coating.
“…Several works have demonstrated that nanoMIPs produced via solid‐phase imprinting can be used as a direct replacement for natural antibodies in diagnostic or bioanalytical assays. [ 199,204–206 ]…”
Section: Different Mip Production Methodsmentioning
confidence: 99%
“…[200][201][202][203] Several works have demonstrated that nanoMIPs produced via solid-phase imprinting can be used as a direct replacement for natural antibodies in diagnostic or bioanalytical assays. [199,[204][205][206] In one such example, the solid-phase approach was used for the manufacture of these imprinted polymers against vancomycin. [207] They were then successfully immobilized on a novel sensor type capable of measuring tiny variations in temperature upon target binding.…”
The World Health Organisation (WHO) reported antimicrobial resistance (AMR) as a global threat comparable to terrorism and climate change. The use of antibiotics in veterinary or clinical practice exerts a selective pressure, which accelerates the emergence of antimicrobial resistance. Therefore, there is a clear need to detect antibiotic residues in complex matrices, such as water, food, and environmental samples, in a fast, selective, cost‐effective, and quantitative manner. Once problematic areas are identified, can extraction of the antibiotics then be carried out to reduce AMR development. Molecularly imprinted polymer (MIPs) are synthetic recognition elements produced through the biomarker of interest being used as a template in order to manufacture tailor‐made ligand selective polymeric recognition sites. They are emerging steadily as a viable alternative to antibiotics, especially given their low‐cost, superior thermal and chemical stability that facilitates on‐site detection, simplified manufacturing process, and avoiding the use of animals in the production process. In this paper, the authors critically review literature from primarily 2010–2020 on rational design approaches used to develop MIPs for sensing and extraction of antibiotics, providing an outlook on crucial issues that need to be tackled to bring MIPs for antibiotic sensing to the market.
“…Several works have demonstrated that nanoMIPs produced via solid‐phase imprinting can be used as a direct replacement for natural antibodies in diagnostic or bioanalytical assays. [ 199,204–206 ]…”
Section: Different Mip Production Methodsmentioning
confidence: 99%
“…[200][201][202][203] Several works have demonstrated that nanoMIPs produced via solid-phase imprinting can be used as a direct replacement for natural antibodies in diagnostic or bioanalytical assays. [199,[204][205][206] In one such example, the solid-phase approach was used for the manufacture of these imprinted polymers against vancomycin. [207] They were then successfully immobilized on a novel sensor type capable of measuring tiny variations in temperature upon target binding.…”
The World Health Organisation (WHO) reported antimicrobial resistance (AMR) as a global threat comparable to terrorism and climate change. The use of antibiotics in veterinary or clinical practice exerts a selective pressure, which accelerates the emergence of antimicrobial resistance. Therefore, there is a clear need to detect antibiotic residues in complex matrices, such as water, food, and environmental samples, in a fast, selective, cost‐effective, and quantitative manner. Once problematic areas are identified, can extraction of the antibiotics then be carried out to reduce AMR development. Molecularly imprinted polymer (MIPs) are synthetic recognition elements produced through the biomarker of interest being used as a template in order to manufacture tailor‐made ligand selective polymeric recognition sites. They are emerging steadily as a viable alternative to antibiotics, especially given their low‐cost, superior thermal and chemical stability that facilitates on‐site detection, simplified manufacturing process, and avoiding the use of animals in the production process. In this paper, the authors critically review literature from primarily 2010–2020 on rational design approaches used to develop MIPs for sensing and extraction of antibiotics, providing an outlook on crucial issues that need to be tackled to bring MIPs for antibiotic sensing to the market.
“…Betlem et al recently introduced an alternative readout approach, using thermistors rather than thermocouples, which simplified analysis by measuring electrical resistance. Furthermore, the use of thermistors offers several advantages such as their low-cost, robustness, and highly sensitive response to small changes in temperature around a fixed base point [ 251 ]. Fig.…”
Highlights
The review aims to summarize recent commercially interesting innovations in MIP-based sensor design.
In addition to sensors, commercially viable assays based on MIPs are analysed and recent advances are summarized and discussed.
The review also analyses how commercial MIP companies could contribute to a next step in the commercialization of MIP-based detection systems.
The review contains a critical analysis and discussion on MIP-based sensors and assay commercialization as well as an outlook/recommendation for the future.
“…New developments are augmenting existing approaches and include the maturation of the field of ‘molecularly imprinted polymers (MIPs)’, giving rise to selective polymer films and nanoparticles with high affinities for their analytes and critically high stability, meaning long-term storage and use of MIP functionalised sensors is becoming highly feasible. An example of an interesting advance in this field is the use of the nano-MIP, which is a nanoparticle bearing high affinity binding sites for the analyte of interest, to detect epidermal growth factor receptor EGFR using a thermistor [67]. Another promising development in improving the durability and longevity of bioreceptors is the ensilication process.…”
Section: Developments In Sensor Chemistrymentioning
The goal of achieving enhanced diagnosis and continuous monitoring of human health has led to a vibrant, dynamic and well-funded field of research in medical sensing and biosensor technologies. The field has many sub-disciplines which focus on different aspects of sensor science; engaging engineers, chemists, biochemists and clinicians, often in interdisciplinary teams. The trends which dominate include the efforts to develop effective point of care tests and implantable/wearable technologies for early diagnosis and continuous monitoring. This review will outline the current state of the art in a number of relevant fields, including device engineering, chemistry, nanoscience and biomolecular detection, and suggest how these advances might be employed to develop effective systems for measuring physiology, detecting infection and monitoring biomarker status in wild animals. Special consideration is also given to the emerging threat of antimicrobial resistance and in the light of the current SARS-CoV-2 outbreak, zoonotic infections. Both of these areas involve significant crossover between animal and human health and are therefore well placed to seed technological developments with applicability to both human and animal health and, more generally, the reviewed technologies have significant potential to find use in the measurement of physiology in wild animals.
This article is part of the theme issue ‘Measuring physiology in free-living animals (Part II)’.
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