Thermally sensitive conductive hydrogel using amphiphilic crosslinker self-assembled carbon nanotube to enhance neurite outgrowth and promote spinal cord regeneration

Lin, Shaofeng; Liu, Yuqing; Hua, Wenxi; Xu, Kaige; Wang, Guobao; Zhong, Wen; Wang, Leyu; Xu, Shaofa; Xing, Malcolm; Qiu, Xiaozhong

doi:10.1039/c5ra20780k

Cited by 26 publications

(20 citation statements)

References 33 publications

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“…CNTs are nanostructures comprised of a cylindrical lattice of carbon atoms arranged either as single-walled or multi-walled nanotubes. CNTs have gained significant interest due to their unique properties, including high compressive and tensile strength, as well as high electrical conductivity [70,72,73,[101][102][103]. CNTs have been shown to be useful for engineering ECHs due to their ability to reduce brittleness and significantly increase electrical conductivity.…”

Section: Carbon Nanotubesmentioning

confidence: 99%

Rational design of microfabricated electroconductive hydrogels for biomedical applications

Walker

Portillo-Lara

Mogadam

et al. 2019

Progress in Polymer Science

156

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Electroconductive hydrogels (ECHs) are highly hydrated three-dimensional (3D) networks generated through the incorporation of conductive polymers, nanoparticles, and other conductive materials into polymeric hydrogels. ECHs combine several advantageous properties of inherently conductive materials with the highly tunable physical and biochemical properties of hydrogels. Recently, the development of biocompatible ECHs has been investigated for various biomedical applications, such as tissue engineering, drug delivery, biosensors, flexible electronics, and other implantable medical devices. Several methods for the synthesis of ECHs have been reported, which include the incorporation of electrically conductive materials such as gold and silver nanoparticles, graphene, and carbon nanotubes, as well as various conductive polymers (CPs), such as polyaniline, polypyrrole, and poly(3,4-ethylenedioxyythiophene) into hydrogel networks. These electroconductive composite hydrogels can be used as scaffolds with high swellability, tunable mechanical properties, and the capability to support cell growth both in vitro and in vivo. Furthermore, recent advancements in microfabrication techniques such as 3D bioprinting, micropatterning, and electrospinning have led to the development of ECHs with biomimetic microarchitectures that reproduce the characteristics of the native extracellular matrix (ECM). The combination of sophisticated synthesis chemistries and modern microfabrication techniques have led to engineer smart ECHs with advanced architectures, geometries, and functionalities that are being increasingly used in drug delivery systems, biosensors, tissue engineering, and soft electronics. In this review, we will summarize different strategies to synthesize conductive biomaterials. We will also discuss the advanced microfabrication techniques used to fabricate ECHs with complex 3D architectures, as well as various biomedical applications of microfabricated ECHs.

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Section: Carbon Nanotubesmentioning

confidence: 99%

Rational design of microfabricated electroconductive hydrogels for biomedical applications

Walker

Portillo-Lara

Mogadam

et al. 2019

Progress in Polymer Science

156

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“…For nerve tissue engineering applications, electro-active scaffolds containing CNTs have been produced by using different polymers and hydrogels. Sang et al produced single-walled carbon nanotube–poly(n-isopropylacrylamide) (SWCNT–PNIPAAm) structures through copolymerization of n-isopropylacrylamide and single-walled carbon nanotubes [ 194 ]. The effects of electrical stimulation on the morphology of SH-SY5Y cells on 2D culture, PNIPAAm hydrogel 3D culture and SWCNT–PNIPAAm hydrogel 3D culture were investigated.…”

Section: Carbon Nanomaterials For Electro-active Scaffoldsmentioning

confidence: 99%

“…No significant difference in 2D groups ( a – f ) and 3D PNIPAAm hydrogel groups ( g – l ) in cell morphology. However, neurite outgrowth was enhanced on SWCNT–PNIPAAm group with electrical stimulation ( m – o ) compared with no electrical stimulation ( p – r ) [ 194 ]. Reproduced with permission from Sang et al, RSC Advances , published by Royal Society of Chemistry, 2016.…”

Section: Figurementioning

confidence: 99%

Carbon Nanomaterials for Electro-Active Structures: A Review

et al. 2020

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The use of electrically conductive materials to impart electrical properties to substrates for cell attachment proliferation and differentiation represents an important strategy in the field of tissue engineering. This paper discusses the concept of electro-active structures and their roles in tissue engineering, accelerating cell proliferation and differentiation, consequently leading to tissue regeneration. The most relevant carbon-based materials used to produce electro-active structures are presented, and their main advantages and limitations are discussed in detail. Particular emphasis is put on the electrically conductive property, material synthesis and their applications on tissue engineering. Different technologies, allowing the fabrication of two-dimensional and three-dimensional structures in a controlled way, are also presented. Finally, challenges for future research are highlighted. This review shows that electrical stimulation plays an important role in modulating the growth of different types of cells. As highlighted, carbon nanomaterials, especially graphene and carbon nanotubes, have great potential for fabricating electro-active structures due to their exceptional electrical and surface properties, opening new routes for more efficient tissue engineering approaches.

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“…Indeed, they found that SWCNT-PNIPAAM hydrogel enhances neurite outgrowth by inducing the electrical stimulation in vitro. Turning to the SCI model, the SWCNT-PNIPAAM hydrogel not only promotes the neural regeneration but also it reduces scars formation in vivo (Sang et al 2016). In another study, Jahromi and coworkers fabricated the PLLA/MWCNT conduits filled with fibrin hydrogel containing SCs and curcumin encapsulated chitosan nanoparticles for sciatic nerve regeneration.…”

Section: Carbon Nanotubesmentioning

confidence: 99%

Nanomaterial integration into the scaffolding materials for nerve tissue engineering: a review

Arzaghi

Adel

Jafari

et al. 2020

Reviews in the Neurosciences

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The nervous system, which consists of a complex network of millions of neurons, is one of the most highly intricate systems in the body. This complex network is responsible for the physiological and cognitive functions of the human body. Following injuries or degenerative diseases, damage to the nervous system is overwhelming because of its complexity and its limited regeneration capacity. However, neural tissue engineering currently has some capacities for repairing nerve deficits and promoting neural regeneration, with more developments in the future. Nevertheless, controlling the guidance of stem cell proliferation and differentiation is a challenging step towards this goal. Nanomaterials have the potential for the guidance of the stem cells towards the neural lineage which can overcome the pitfalls of the classical methods since they provide a unique microenvironment that facilitates cell–matrix and cell–cell interaction, and they can manipulate the cell signaling mechanisms to control stem cells’ fate. In this article, the suitable cell sources and microenvironment cues for neuronal tissue engineering were examined. Afterward, the nanomaterials that impact stem cell proliferation and differentiation towards neuronal lineage were reviewed.

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Thermally sensitive conductive hydrogel using amphiphilic crosslinker self-assembled carbon nanotube to enhance neurite outgrowth and promote spinal cord regeneration

Abstract: We studied a SWNT–PNIPAAM hydrogel for its potential role in the growth of SH-SY5Y cells and found it can enhance neurite outgrowth when adding electrical stimulation in vitro.

Cited by 26 publications

References 33 publications

Rational design of microfabricated electroconductive hydrogels for biomedical applications

Rational design of microfabricated electroconductive hydrogels for biomedical applications

Carbon Nanomaterials for Electro-Active Structures: A Review

Nanomaterial integration into the scaffolding materials for nerve tissue engineering: a review

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