Thermal treatment enhances the stability and biological activity of a truncated tilapia somatotropin contained in Pichia pastoris culture supernatant

Acosta, Jannel; Ruiz, Odalys; Carpio, Yamila; Morales, Reynold; Águila, Julio; Valdés, Jorge Hernández; Martínez, Eduardo Justo; Estrada, Mario Pablo

doi:10.1016/j.jbiotec.2010.11.017

Cited by 2 publications

(2 citation statements)

References 33 publications

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“…Additionally, the results are similar to those found in larvae of tilapia (Oreochromis sp.) treated with GH tilapia, lacking the 46 last amino acids of the C-terminal, through dips, where the only significant difference was found in antiprotease activity and lectins titer within 30 days of treatment (Acosta et al 2011).…”

Section: Discussionmentioning

confidence: 96%

“…In addition, administration by immersion baths of neuropeptide Y recombinant African catfish larvae (C. gariepinus) increased the concentration of reduced glutathione and SOD activity, without producing any effect on the activity of catalase (Carpio et al 2007). Also, in larvae of tilapia treated with recombinant tilapia, GH was increased in the activity of SOD and catalase (Acosta et al 2011).…”

Section: Discussionmentioning

confidence: 97%

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A novel GH secretagogue, A233, exhibits enhanced growth activity and innate immune system stimulation in teleosts fish

Martı́nez

Ubieta

Herrera

et al. 2012

Journal of Endocrinology

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In teleosts fish, secretion of GH is regulated by several hypothalamic factors that are influenced by the physiological state of the animal. There is an interaction between immune and endocrine systems through hormones and cytokines. GH in fish is involved in many physiological processes that are not overtly growth related, such as saltwater osmoregulation, antifreeze synthesis, and the regulation of sexual maturation and immune functions. This study was conducted to characterize a decapeptide compound A233 (GKFDLSPEHQ) designed by molecular modeling to evaluate its function as a GH secretagogue (GHS). In pituitary cell culture, the peptide A233 induces GH secretion and it is also able to increase superoxide production in tilapia head-kidney leukocyte cultures. This effect is blocked by preincubation with the GHS receptor antagonist [D-Lys 3 ]-GHRP6. Immunoneutralization of GH by addition of anti-tilapia GH monoclonal antibody blocked the stimulatory effect of A233 on superoxide production. These experiments propose a GH-mediated mechanism for the action of A233. The in vivo biological action of the decapeptide was also demonstrated for growth stimulation in goldfish and tilapia larvae (P!0 . 001). Superoxide dismutase levels, antiprotease activity, and lectin titer were enhanced in tilapia larvae treated with this novel molecule. The decapeptide A233 designed by molecular modeling is able to function as a GHS in teleosts and enhance parameters of the innate immune system in the fish larvae.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 97%