Thermal stress, thermal safety margins and acclimation capacity in tropical shallow waters—An experimental approach testing multiple end-points in two common fish

Madeira, Carolina; Mendonça, Vanessa; Leal, Miguel C.; Flores, Augusto A. V.; Cabral, Henrique N.; Diniz, Mário; Vinagre, Catarina

doi:10.1016/j.ecolind.2017.05.050

Cited by 32 publications

(25 citation statements)

References 93 publications

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“…The results of the present study indicate that the “antioxidant activity” process can be triggered as part of the HSF1 regulation in response to heat shock. This finding is supported by the results of Madeira et al [57] regarding two common fish species living in an environment commonly exposed to high temperatures.…”

Section: Discussionsupporting

confidence: 84%

RNAi based transcriptome suggests genes potentially regulated by HSF1 in the Pacific oyster Crassostrea gigas under thermal stress

Liu

Huang

et al. 2019

BMC Genomics

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Background The Pacific oyster Crassostrea gigas is an important fishery resource that is sensitive to temperature fluctuations. Thus, it has evolved a protection mechanism against heat stress by increasing the expression of the gene coding for heat shock protein (HSP) 70 under elevated temperatures. In other animals, heat shock response is a transcriptional response driven by the heat shock transcription factor 1 ( HSF1 ) and thermal stress can trigger HSP70 expression to protect the organism via HSF1 . However, the regulatory relationship between HSF1 and HSP remains unclear in Pacific oyster. Therefore, in the present study, we examined the transcriptomic response of several to thermal stress following HSF1 interference. Results We identified 150 genes responsive to heat shock including seven HSP genes, six of which belonging to the group of 17 HSP genes enriched in response to heat shock, according to weighted gene co-expression network analysis (WGCNA). The other gene was enriched in the module correlated with HSF1 interference. In addition, we found 48 and 47 genes that were upregulated and downregulated by HSF1 in response to heat shock, respectively. In the upregulated genes, we identified one HSP70 potentially regulated by HSF1 in response to heat shock. Furthermore, based on differentially expressed genes and WGCNA analyses, we found that the hypoxia signaling pathway was enriched under heat shock conditions. Five genes were then selected to detect dynamic changes through time. The results suggested that gene expression was correlated with HSF1 expression. The regulation of HSP70 by HSF1 was preliminarily confirmed by binding site predictions and by a dual luciferase assay. Conclusions Our results revealed that the expression of HSP70 and HSP20 was initially triggered after 2 h of heat shock, and one of the HSP70 genes was potentially regulated by HSF1 . From these results, it is evident that not all heat-inducible genes were triggered simultaneously in response to heat shock stress. Overall, the results revealed a possible HSF1–HSP regulatory relationship in Pacific oyster, providing valuable information on the mechanisms of thermal tolerance in this commercially important oyster. Electronic supplementary material The online version of this article (10.1186/s12864-019-6003-8) contains supplementary material, which is available to authorized use...

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Section: Discussionsupporting

confidence: 84%

RNAi based transcriptome suggests genes potentially regulated by HSF1 in the Pacific oyster Crassostrea gigas under thermal stress

Liu

Huang

et al. 2019

BMC Genomics

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“…A milder, non‐acutely, thermal stress may decrease physiological or behavioral performance in the temperature fitness curve, which drops relatively faster above the optimal temperature, T opt , at which performance maximizes fitness. The thermal safety margin (TSM) index, defined in different ways (Deutsch et al ., 2008; Huey et al ., 2009; Sunday et al ., 2014; Sinclair et al ., 2016; Madeira et al ., 2017), will measure the amount of warming possible before the average environmental temperatures (e.g. T mean , T median ) reach or exceed the optimal temperatures.…”

Section: Introductionmentioning

confidence: 99%

Vulnerability to warming in a desert amphibian tadpole community: the role of interpopulational variation

et al. 2020

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Current assessments of organismal vulnerability to global warming are focusing on physiological trait‐based indices that may allow biologically sounding estimates of heating risk at the local scale. However, intraspecific variability in both exposure and physiological performance may determine large heterogeneity in the distribution of heating risks through the overall species range compromising whole species risk assessments. We examine intra‐ and interspecific variability in two vulnerability indexes: warming tolerances (WT), or the extent that maximum temperatures (T max) reaching upper thermal tolerances (CTmax); and thermal safety margin (TSM), or the magnitude that average environmental temperatures (T mean) exceeding the selected or preferred temperatures (T sel), in a subtropical warm amphibian tadpole community, at the Monte Desert ecoregion, Argentina. Tadpole populations breeds in temporary ponds and permanent streams that exhibit high thermal heterogeneity. Those populations and species living in hot water bodies, although exhibiting higher CTmax, have lower WT and are more prone to suffer acute heat impacts, thus confirming the prediction of higher thermal risk in ectotherms exposed to higher temperatures. However, these hot pond breeders, although living closer to their CTmax, also preferred high temperatures, showing higher TSMs and being actually exposed to a lower proportion of stressful temperatures than cool selecting populations. Both WT and TSM indexes have significant interpopulational variation. Thus, our findings suggest that attempts to build mechanistic models to forecast species vulnerability to heat stress due to climate change have to include physiological variation among populations.

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“…For example, local changes in biomass density can be a result of movement, local population growth, age cohorts beyond our two maturity categories, changes in average body size (Laurel et al., 2007; Shackell et al., 2010), or effects of fishing not captured by the metrics of total catch or hours fished. Indeed, some of the hypothesized effects of warming climate and lower DO on fishes include higher metabolism and ability to store fat, reduced productivity, and slower growth resulting in generally smaller fish (Klein et al., 2017; Madeira et al., 2017). Furthermore, changes in local density may be correlated with climate, not because of groundfish thermal preference, but because groundfish seek prey or avoid predators that have themselves shifted their distribution in response to climate.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, some of the hypothesized effects of warming climate and lower DO on fishes include higher metabolism and ability to store fat, reduced productivity, and slower growth resulting in generally smaller fish (Klein et al, 2017;Madeira et al, 2017). Furthermore, changes in local density may be correlated with climate, not because of groundfish thermal preference, but because groundfish seek prey or avoid predators that have themselves shifted their distribution in response to climate.…”

Section: Limitations and Implicationsmentioning

confidence: 99%

Contrasting climate velocity impacts in warm and cool locations show that effects of marine warming are worse in already warmer temperate waters

et al. 2021

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Thermal stress, thermal safety margins and acclimation capacity in tropical shallow waters—An experimental approach testing multiple end-points in two common fish

Cited by 32 publications

References 93 publications

RNAi based transcriptome suggests genes potentially regulated by HSF1 in the Pacific oyster Crassostrea gigas under thermal stress

RNAi based transcriptome suggests genes potentially regulated by HSF1 in the Pacific oyster Crassostrea gigas under thermal stress

Vulnerability to warming in a desert amphibian tadpole community: the role of interpopulational variation

Contrasting climate velocity impacts in warm and cool locations show that effects of marine warming are worse in already warmer temperate waters

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