2011
DOI: 10.1590/s0104-66322011000300002
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Thermal stability of the immobilized fructosyltransferase from Rhodotorula sp

Abstract: -The thermal stability of the extracellular fructosyltransferase (FTase) from Rhodotorula sp., recovered from cultivation medium by ethanol precipitation and immobilized onto niobium ore, was studied by Arrhenius plot, half-life profile, half-inactivation temperature (T 50 ) and thermodynamic parameters. The Arrhenius plot showed two different behaviors with different deactivation energies (E ad ) only after immobilization, the transition occurring in the temperature interval between 51 and 52°C. T 50 for the … Show more

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Cited by 27 publications
(34 citation statements)
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“…LEB-V10 yeast and verified a loss of activity up to 61ºC. They suggest that the enzyme instability at higher temperatures led to the loss of transfructosylation capacity (Aguiar-Oliveira & Maugeri, 2011). …”
Section: Effect Of Ph and Temperature On Fructooligosaccharide Producmentioning
confidence: 82%
“…LEB-V10 yeast and verified a loss of activity up to 61ºC. They suggest that the enzyme instability at higher temperatures led to the loss of transfructosylation capacity (Aguiar-Oliveira & Maugeri, 2011). …”
Section: Effect Of Ph and Temperature On Fructooligosaccharide Producmentioning
confidence: 82%
“…Based on the methodologies described and employed in previous studies (Hernalsteens and Maugeri ; Aguiar‐Oliveira and Maugeri ), Rhodotorula sp. LEB‐V10 was cultivated in a medium containing sugar cane molasses and corn steep liquor.…”
Section: Methodsmentioning
confidence: 99%
“…The standard conditions for FOS synthesis from commercial sucrose were based on previous works (Hernalsteens and Maugeri ; Aguiar‐Oliveira and Maugeri ): 50% (w/v) of sucrose in sodium acetate buffer (50 mM) at pH 4.5 or 6.0, 50C and 6 U i /mL. For comparison, syntheses were performed with the standard immobilized enzyme (without any additions to the dispersion medium) under the same standard conditions.…”
Section: Methodsmentioning
confidence: 99%
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