Thermal response of domains in cardiolipin content bilayers

Doménech, Òscar; Morros, Antoni; Cabañas, Miquel E.; Montero, M. Teresa; Hernández-Borrell, Jordi

doi:10.1016/j.ultramic.2007.04.009

Cited by 26 publications

(30 citation statements)

References 29 publications

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“…diverse molecular species of cardiolipin have stimulated the investigation of numerous analytical and mathematical approaches to determine the importance of cardiolipin in biological function. Coarse-grained molecular dynamics, thermodynamics and thermal response, atomic force microscopy, and atomic-scale simulations have all been used to characterize structural orientation and interaction of cardiolipin in membrane dynamics (55)(56)(57)(58)(59)(60). More recently, mathematical models using linear algebraic representation have been used to explain the acyl selectivity of cardiolipin remodeling based on free energies ( 61 ).…”

Section: Discussionmentioning

confidence: 99%

Dynamic simulation of cardiolipin remodeling: greasing the wheels for an interpretative approach to lipidomics

Kiebish

Bell

Yang

et al. 2010

Journal of Lipid Research

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Lipids are critical for the regulation and control of cellular function, membrane dynamics, and signal transduction. The systematic identifi cation and analysis of all cellular lipids in a biological sample is embodied by the rapidly developing fi eld of lipidomics, which has been enabled by recent advances in mass spectrometry ( 1-3 ). A multidimensional MS-based shotgun lipidomics (MDMS-SL) approach for analysis of cellular lipidomes has been demonstrated as one of the most systematic and quantitative high-throughput platforms for global lipidomics capable of analyzing thousands of lipid molecular species directly from the lipid extracts of biological tissues ( 4, 5 ). Analysis of lipidomic data requires extensive use of bioinformatics and computational algorithms for the accurate identifi cation and quantifi cation of lipid molecular species and classes ( 5-9 ). However, most advances in bioinformatics in lipidomics have largely focused on lipid identifi cation rather than interpretation of alterations in biological function resulting in adaptive or pathologic changes in lipid metabolism ( 10 ). Thus, development of bioinformatic and systems biology approaches for the interpretation of lipidomic networks would signifi cantly advance the understanding of the roles of lipids in biological systems ( 3 ).Cardiolipin is a complex polyglycerophospholipid found almost exclusively in mitochondrial membranes of eukaryotic organisms ( 11 ). Cardiolipin is intricately involved in the effi cient production of ATP through utilizing the proton gradient as well as maintaining mitochondrial functionality ( 12-14 ). The diversity of cardiolipin molecular species varies markedly between tissues as well as among Abstract Cardiolipin is a class of mitochondrial specifi c phospholipid, which is intricately involved in mitochondrial functionality. Differences in cardiolipin species exist in a variety of tissues and diseases. It has been demonstrated that the cardiolipin profi le is a key modulator of the functions of many mitochondrial proteins. However, the chemical mechanism(s) leading to normal and/or pathological distribution of cardiolipin species remain elusive. Herein, we describe a novel approach for investigating the molecular mechanism of cardiolipin remodeling through a dynamic simulation. This approach applied data from shotgun lipidomic analyses of the heart, liver, brain, and lung mitochondrial lipidomes to model cardiolipin remodeling, including relative content, regiospecifi city, and isomeric composition of cardiolipin species. Generated cardiolipin profi les were nearly identical to those determined by shotgun lipidomics. Importantly, the simulated isomeric compositions of cardiolipin species were further substantiated through product ion analysis. Finally, unique enzymatic activities involved in cardiolipin remodeling were assessed from the parameters used in the dynamic simulation of cardiolipin profi les. Collectively, we described, verifi ed, and demonstrated a novel approach by integrating both lipidomic ana...

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Section: Discussionmentioning

confidence: 99%

Dynamic simulation of cardiolipin remodeling: greasing the wheels for an interpretative approach to lipidomics

Kiebish

Bell

Yang

et al. 2010

Journal of Lipid Research

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“…We hypothesized that monocaprylate's interaction with the membrane involves free CL in the L d phase because CL is known to form domains in lipid membranes (12,14). We therefore tested the effect on SLBs made from combinations of pure phospholipids (PE and PG) with or without CL.…”

Section: Fig 1 Number Of Viable S Xylosus (ᮀ) E Coli (O) and Z Bmentioning

confidence: 99%

“…In prokaryotes, the heterogeneous distribution of phospholipids in the cell membrane results in formation of domains that are considered analogous to the eukaryotic lipid rafts (16,22). Bacterial membranes encompass a solid ordered (S o ) lipid phase enriched in CL and a liquid disordered (L d ) lipid phase (13,14,23,35). Using the CL-binding stain 10-N-nonyl acridine orange (NAO) on E. coli "minicells" that are mainly composed of phospholipids found at the cell poles, it was documented that CLcontaining domains form at the poles and septum of rod-shaped bacteria, and that these are important for several cellular functions (22,27,31).…”

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confidence: 99%

Antimicrobial Mechanism of Monocaprylate

Hyldgaard

Sutherland

Sundh

et al. 2012

Appl Environ Microbiol

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ABSTRACTMonoglyceride esters of fatty acids occur naturally and encompass a broad spectrum of antimicrobial activity. Monocaprylate is generally regarded as safe (GRAS) and can function both as an emulsifier and as a preservative in food. However, knowledge about its mode of action is lacking. The aim of this study was therefore to elucidate the mechanism behind monocaprylate's antimicrobial effect. The cause of cell death inEscherichia coli,Staphylococcus xylosus, andZygosaccharomyces bailiiwas investigated by examining monocaprylate's effect on cell structure, membrane integrity, and its interaction with model membranes. Changes in cell structure were visible by atomic force microscopy (AFM), and propidium iodide staining showed membrane disruption, indicating the membrane as a site of action. This indication was confirmed by measuring calcein leakage from membrane vesicles exposed to monocaprylate. AFM imaging of supported lipid bilayers visualized the integration of monocaprylate into the liquid disordered, and not the solid ordered, phase of the membrane. The integration of monocaprylate was confirmed by quartz crystal microbalance measurements, showing an abrupt increase in mass and hydration of the membrane after exposure to monocaprylate above a threshold concentration. We hypothesize that monocaprylate destabilizes membranes by increasing membrane fluidity and the number of phase boundary defects. The sensitivity of cells to monocaprylate will therefore depend on the lipid composition, fluidity, and curvature of the membrane.

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“…Previously the role of CL in modulating membrane properties and membrane integrity has been probed using biophysics model membranes, including monolayer, bilayer, and vesicle systems. Model membrane studies determined that the incorporation of CL strongly affected the thermodynamic properties of lipid monolayers (15,16) and bilayers (15,(17)(18)(19). Monolayer analyses indicate that a mixture of EPC (egg phosphatidylcholine) and CL in specific mixed ratios and at specific pressures is thermodynamically stable, even though the apparent area compressibility modulus and the lysis tension of vesicle structures decreases with increasing CL content.…”

Section: Introductionmentioning

confidence: 99%

“…Such an effect would likely decrease the energy required to create folds in the inner mitochondrial membrane. Due to its chemical structure, CL may form micellar, lamellar, and hexagonal states in an aqueous dispersion, depending on the pH and ionic strength of the environment (17)(18)(19)(20). The abundance of high curvature zones in mitochondrial cristae might also be attributed to the intrinsic curvature of the CL molecule (7,21).…”

Section: Introductionmentioning

confidence: 99%

Effects of Cardiolipin on Membrane Morphology: A Langmuir Monolayer Study

Phan

Shin

2015

Biophysical Journal

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Cardiolipin (CL) is a complex phospholipid that is specifically found in mitochondria. Owing to the association of the CL levels with mitochondrial physiopathology such as in Parkinson's disease, we study the molecular effect of CL on membrane organization using model Langmuir monolayer, fluorescence microscopy, and x-ray reflectivity. We find that the liquid-expanded phase in membranes increases with increasing CL concentration, indicating an increase in the elasticity of the mixed membrane. The Gibbs excess free energy of mixing indicates that the binary monolayer composed of CL and DPPC is most thermodynamically stable at F CL ¼ 10 mol %, and the stability is enhanced when the surface pressure is increased. Additionally, when F CL is small, the expansion of the membrane with increasing CL content was slower at higher surface pressure. These abnormal results are indicative of a folding structure being present before a collapsing structure, which was confirmed by using fluorescence microscopy and was characterized by using x-ray reflectivity with the electron density profile along the membrane's surface normal.

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Thermal response of domains in cardiolipin content bilayers

Cited by 26 publications

References 29 publications

Dynamic simulation of cardiolipin remodeling: greasing the wheels for an interpretative approach to lipidomics

Dynamic simulation of cardiolipin remodeling: greasing the wheels for an interpretative approach to lipidomics

Antimicrobial Mechanism of Monocaprylate

Effects of Cardiolipin on Membrane Morphology: A Langmuir Monolayer Study

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